A5029952418- Reproductive Biology and Fertility
- Tissue Engineering and Regenerative Medicine
- FOXO transcription factor regulation
- Ovarian function and disorders
- Mesenchymal stem cell research
- Epigenetics and DNA Methylation
- Reproductive System and Pregnancy
- Tendon Structure and Treatment
- Colorectal Cancer Treatments and Studies
- Melanoma and MAPK Pathways
- Genetics, Aging, and Longevity in Model Organisms
- MicroRNA in disease regulation
- Autophagy in Disease and Therapy
- Histone Deacetylase Inhibitors Research
- Sperm and Testicular Function
- RNA modifications and cancer
- Animal Virus Infections Studies
- 3D Printing in Biomedical Research
- Mitochondrial Function and Pathology
- Corneal Surgery and Treatments
- Cancer-related gene regulation
- Hippo pathway signaling and YAP/TAZ
- Heat shock proteins research
- Molecular Communication and Nanonetworks
- Cell death mechanisms and regulation
University of Teramo
2020-2025
Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale
2019-2020
University of Bari Aldo Moro
2015-2018
Istituto Tumori Bari
2015
Istituti di Ricovero e Cura a Carattere Scientifico
2015
Medical Genetics Center
2015
Mario Negri Sud Foundation
2010-2013
SMYD3 is a histone lysine methyltransferase that plays an important role in transcriptional activation as member of RNA polymerase complex, and its oncogenic has been described different cancer types. We studied the expression activity preclinical model colorectal (CRC) found it strongly upregulated throughout tumorigenesis both at mRNA protein level. Our results also showed RNAi‐mediated ablation impairs CRC cell proliferation indicating required for proper growth. These data, together with...
Postzygotic mutations of the PIK3CA [phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha] gene constitutively activate PI3K/AKT/mTOR pathway in PIK3CA-related overgrowth spectrum (PROS) patients, causing congenital mosaic tissue that even multiple surgeries cannot solve. mTOR inhibitors are empirically tested and given for compassionate use these patients. PROS patients could be ideal candidates enrolment trials with PI3K/AKT inhibitors, considering "clean" cellular...
The HSF and FOXO families of transcription factors play evolutionarily conserved roles in stress resistance lifespan. In humans, the rs2802292 G-allele at FOXO3 locus has been associated with longevity all human populations tested; moreover, its copy number correlated reduced frequency age-related diseases centenarians. At molecular level, intronic increased expression FOXO3, suggesting that intron 2 may represent a regulatory region. Here we show 90-bp sequence around single nucleotide...
Amniotic epithelial cells (AECs) exhibit significant immunomodulatory and pro-regenerative properties, largely due to their intrinsic paracrine functions that are currently harnessed through the collection of secretomes. While there is increasing evidence role bioactive components freely secreted or carried by exosomes, cargo AEC microvesicles (MVs) crosstalk with immune remains be fully explored. We showed under conditions in response LPS, AEC-derived MV carries such as lipid-mediated...
Abstract While aberrant cancer cell growth is frequently associated with altered biochemical metabolism, normal mitochondrial functions are usually preserved and necessary for full malignant transformation. The transcription factor FoxO3A a key determinant of homeostasis, playing dual role in survival/death response to metabolic stress therapeutics. We recently described novel arm the AMPK-FoxO3A axis cells upon nutrient shortage. Here, we show that metabolically stressed cells, recruited...
Prostate cancer (PCa) is the second leading cause of cancer-related death in men; however, molecular mechanisms to its development and progression are not yet fully elucidated. Of note, it has been recently shown that conditional stk11 knockout mice develop atypical hyperplasia prostate intraepithelial neoplasia (PIN). We reported an inverse correlation between activity STK11/AMPK pathway MAPK/p38 cascade HIF1A-dependent malignancies. Furthermore, overactivation was detected benign...
Abstract Prompt identification of the causative pathogen an infectious disease is essential for choice treatment or preventive measures. In this perspective, nucleic acids purified from brain tissue a dog succumbed after severe neurological signs were processed with MinION (Oxford Nanopore Technologies, Oxford UK) sequencing technology. Canine distemper virus (CDV) sequence reads detected. Subsequently, specific molecular test and immunohistochemistry used to confirm presence CDV RNA...
Amniotic epithelial stem cells (AECs) are largely studied for their pro-regenerative properties. However, it remains undetermined if low oxygen (O2) levels that AECs experience in vivo can be of value maintaining biological properties after isolation. To this aim, the present study has been designed to evaluate effects a hypoxia-mimetic agent, cobalt chloride (CoCl2), on AECs' stemness and angiogenic activities. First, CoCl2 dose-effect was performed select concentration able induce hypoxia,...
The application of biomaterials on immune regenerative strategies to deal with unsolved pathologies is getting attention in the field tissue engineering. In this context, graphene oxide (GO) has been proposed as an immune-mimetic material largely used for developing stem cell-based therapies, since it shown influence cell behavior and modulate their response. Similarly, amniotic epithelial cells (AECs) are increasing clinical interest source due great plasticity immunomodulatory paracrine...
Tendinopathies are at the frontier of advanced responses to health challenges and sectoral policy targets. Cell-based therapy holds great promise for tendon disorder resolution. To verify role stepwise trans-differentiation amniotic epithelial stem cells (AECs) in regeneration, present research three different AEC subsets displaying an (eAECs), mesenchymal (mAECs), tendon-like (tdAECs) phenotype were allotransplanted a validated experimental sheep Achilles injury model. Tissue healing was...
There is high clinical demand for the resolution of tendinopathies, which affect mainly adult individuals and animals. Tendon damage during lifetime not as effective in earlier stages where complete restoration tendon structure property occurs. However, molecular mechanisms underlying regeneration remain unknown, limiting development targeted therapies. The research aim was to draw a comparative map molecules that control tenogenesis exploit systems biology model their signaling cascades...