A5046310225- Salmonella and Campylobacter epidemiology
- Listeria monocytogenes in Food Safety
- Antimicrobial Resistance in Staphylococcus
- Insect and Pesticide Research
- Probiotics and Fermented Foods
- Plant and animal studies
- Insect and Arachnid Ecology and Behavior
- Microbial Inactivation Methods
- Bacterial Identification and Susceptibility Testing
- Gut microbiota and health
- Viral gastroenteritis research and epidemiology
- Bacteriophages and microbial interactions
- Genomics and Phylogenetic Studies
- Oral microbiology and periodontitis research
- Animal Virus Infections Studies
- Streptococcal Infections and Treatments
- Brucella: diagnosis, epidemiology, treatment
- Biosensors and Analytical Detection
- Microbial infections and disease research
- Microbial Metabolism and Applications
- Food Safety and Hygiene
- Clostridium difficile and Clostridium perfringens research
- Infectious Diseases and Mycology
- Veterinary medicine and infectious diseases
- Antimicrobial agents and applications
University of Ljubljana
2014-2025
Institute of Oncology Ljubljana
2025
In a previous study characterizing Campylobacter strains deficient in selenium metabolism, 50 were found to be similar to, but distinct from, the selenonegative species lanienae . Initial characterization based on multilocus sequence typing and phylogeny of set 20 core genes determined that these form three putative taxa within cluster. A polyphasic was undertaken here further clarify their taxonomic position genus. The underwent phylogenetic analyses sequences 16S rRNA gene an expanded 330...
ABSTRACT Listeria monocytogenes presents a significant concern for the food industry due to its ability persist in processing environment. One of factors contributing persistence is decreased sensitivity disinfectants. Our objective was assess diversity L. disinfectants by testing response 1,671 L . isolates quaternary ammonium compounds (QACs) and 414 peracetic acid (PAA) using broth microdilution growth curve analysis assays, respectively, categorize into sensitive tolerant. A high...
The emergence of antimicrobial-resistant and virulent enterococci is a major public health concern. While are commonly found in food animal origin, the knowledge on their zoonotic potential limited. aim this study was to determine compare antimicrobial susceptibility virulence traits Enterococcus faecalis faecium isolates from human clinical specimens retail red meat Slovenia. A total 242 were investigated: 101 humans (71 E. faecalis, 30 faecium) 141 fresh beef pork (120 21 faecium). 12...
Abstract Background Listeria monocytogenes is the causative agent of listeriosis, a serious disease affecting both animals and humans. Here, multilocus sequence typing (MLST) was used to characterize genetic diversity strains isolated from natural environment animal clinical cases in Europe. The prevalence clonal complexes (CCs) obtained compared according ( i ) origin isolation – vs. ii form listeriosis rhombencephalitis abortion. To this aim, two datasets were constructed. dataset...
Flavobacterium psychrophilum and branchiophilum are important fish pathogens that cause considerable economic losses in freshwater aquaculture worldwide. Their antimicrobial resistance (AMR) profiles were evaluated using the latest Clinical Laboratory Standards Institute broth microdilution method, which was adjusted for F. to achieve growth, whole-genome sequencing (WGS). A total of 51 isolates eight from Slovenian farmed rainbow trout underwent phenotypic susceptibility testing. Overall,...
This review paper briefly overviews various methods used to detect and identify Salmonella, a common foodborne zoonotic pathogen in veterinary medicine. Salmonella bacteria is mainly found food products and, when ingested, causes severe gastrointestinal symptoms. Due the ongoing presence of pathogenic production systems, it poses serious public health threat. Therefore, constant need remains enhance identification detection capable identifying this preventing outbreaks. The conventional...
Campylobacteriosis is the most frequently reported bacterial food-borne illness in European Union and contaminated broiler meat considered important source of infection humans. The aim present study was to evaluate real-time PCR (qPCR) digital (dPCR) for quantification Campylobacter jejuni 75 neck-skin samples collected from a poultry slaughterhouse, compare them with ISO 10272-2 standard plate count method. For qPCR curve, C. jejuni-negative were spiked suspension known number cells....
Staphylococcus pseudintermedius is a common cause of skin and soft tissue infections in dogs but can also cats humans. The frequency methicillin-resistant S. (MRSP) strains increasing worldwide. Here, we obtained 43 MRSP isolates from (n = 41), one cat 1) the small animal clinic environment Slovenia period 2008–2018, which underwent whole-genome sequencing (WGS) antimicrobial susceptibility testing. Five sequence types (STs) were identified, with ST71 (32/43) ST551 (8/43) being predominant....
Abstract Listeria monocytogenes ( Lm ) is a ubiquitous bacterium that causes listeriosis, serious foodborne illness. In the nature-to-human transmission route, can prosper in various ecological niches. Soil and decaying organic matter are its primary reservoirs. Certain clonal complexes (CCs) over-represented food production represent challenge to safety. To gain new understanding of adaptation mechanisms food, genetic background strains found animals environment should be investigated...
Salmonella enterica subsp. serovar Infantis is the most prevalent found in broilers and broiler meat among top five serovars responsible for human infections Europe. In 2008, a multidrug-resistant S. isolate emerged Israel with mosaic megaplasmid named pESI, associated increased virulence, biofilm formation, multidrug resistance. Since then, clones pESI-like plasmids have been reported worldwide, replacing pESI-free clones. Here, we typed 161 isolates of poultry (n = 133) clinical 28) origin...
Aims An extensive source investigation was conducted on a dairy farm with neurolisteriosis and subclinical mastitis cases to identify infection potential transmission routes of Listeria monocytogenes. Methods Results A total 36 L. monocytogenes isolates were obtained from animal clinical (neurolisteriosis udder infection) the environment (silage, faeces, water). Isolates typed using pulsed-field gel electrophoresis (PFGE) whole-genome sequencing (WGS). Their virulence assessed gentamicin...
Six Helicobacter-like isolates were recovered from 15 gastric mucosa samples of red foxes (Vulpes vulpes) shot by hunters in the surroundings Ljubljana, Slovenia. Gram-negative, tightly coiled, intensely motile, 7-15 µm long and ≤1 wide bacteria grew on biphasic blood agar plates. By using a genus-specific polymerase chain reaction (PCR), all confirmed as Helicobacter sp. subsequently subjected to whole-genome sequencing (WGS). Five showed genome-wide average nucleotide identity (ANI) value...
In humans, transmission of bacteria causing fatal sepsis in the neonates through mother's milk has been reported. dogs, it is believed that from canine are not primary cause neonatal infections. Staphylococcus pseudintermedius colonizing skin and mucocutaneous junctions adult dogs can act as an opportunistic pathogen. This bacterium was previously isolated and, although, its dam's to newborn puppies a suggested, this hypothesis confirmed. A 4.5-year-old healthy Boston terrier dam had...
Porcine epidemic diarrhea virus (PEDV) was detected for the first time in Slovenia January 2015. The complete genome sequence of PEDV strain SLO/JH-11/2015, obtained from a fecal sample fattening pig with September 2015, is closely related to recently European strains.
Paenibacillus larvae causes the American foulbrood (AFB), a highly contagious and devastating disease of honeybees. Whole-genome sequencing (WGS) has been increasingly used in bacterial pathogen typing, but rarely applied to study epidemiology P. . To this end, we 125 genomes representative species-wide diversity construct stable whole-genome multilocus sequence typing (wgMLST) scheme consisting 5745 loci. A total 51 isolates originating from AFB outbreaks Slovenia were assess...
Paenibacillus larvae is the causative agent of American foulbrood (AFB), a devastating disease honeybees. P. spore counts in bee-related samples correlate with presence AFB symptoms and may, therefore, be used to identify at-risk colonies. Here, we constructed TaqMan-based real-time PCR (qPCR) assay targeting single-copy chromosomal metalloproteinase gene for reliable quantification larvae. The was calibrated using digital (dPCR) allow absolute spores honey hive debris samples. limits...
Listeria monocytogenes is an important human pathogen with a high mortality rate. Consumption of contaminated ready-to-eat food the main mode transmission to humans. Disinfectant-tolerant L. have emerged, which are believed increased persistence potential. Elucidating mechanisms disinfectant tolerance has been focus previous studies using pure cultures. A limitation such approach difficulty identify strains reduced susceptibility due inter-strain variation and need screen large numbers...
ABSTRACT Listeria monocytogenes is a significant concern for the food industry due to its ability persist in processing environment. Decreased susceptibility disinfectants one of factors that contribute persistence L. . The objective this study was explore diversity quaternary ammonium compounds (QACs) using 1,671 isolates. This used determine phenotype-genotype concordance and characterize genomes QAC sensitive tolerant isolates stress resistance, virulence plasmid replicon genes....
During the 2021 European Food Safety Authority coordinated harmonized monitoring of antimicrobial resistance in Campylobacter species Slovenia, five Campylobacter-like strains were cultured from caeca a total 104 domestic pigs that could not be identified using standard-prescribed biochemical tests or MALDI-TOF MS. The isolates obtained standard ISO 10272 procedure for isolation thermotolerant with prolonged cultivation time. Small colonies observed on mCCDA and CASA agar plates after 2-4...
Paenibacillus larvae is the causative agent of American foulbrood (AFB), a fatal disease honeybee brood. Here, we obtained 506 P. isolates originating from honey or brood samples and different geographic regions Slovenia in period 2017–2019. In first part study, conducted ERIC-PCR typing to assess frequency ERIC types Slovenia. Capillary electrophoresis was used for analysis patterns, revealing good separation efficiency enabling easy lane-to-lane comparisons. II predominant type (70.2%),...
Livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) represents a concern in both human and veterinary medicine. The aim of this study was to investigate potential LA-MRSA transmission between animals humans rural settings. To aim, designed include 14 farms Slovenia, which were selected on the basis farmer (initial patient) with confirmed infection regular animal contacts. On all farms, initial patients, their household members, barn environment analysed for presence...
Pigs were identified as the most important reservoir of livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA), mostly belonging to emergent zoonotic clonal complex (CC) 398. Here, we investigated presence MRSA in sows and piglets over a period several months two pig farms (intensive farm A family-run B). Isolates underwent antimicrobial susceptibility testing, PCR characterization spa typing. We collected 280 samples, namely 206 nasal swabs from pigs 74 environmental...
Seven Helicobacter-like isolates were cultured from caecal contents of 100 domestic pigs (Sus scrofa domesticus) sampled as part the EFSA-coordinated harmonized monitoring antimicrobial resistance in Campylobacter sp. 2015. The bacteria isolated using standard ISO 10272 procedure for isolation thermotolerant with extended incubation time and formed small, grey, moist flat colonies a metallic sheen (small Campylobacter-like colonies) on modified Charcoal-Cefoperazone-Deoxycholate Agar (mCCDA)...
Brucella spp. that cause marine brucellosis are becoming more important, as the disease appears to be widespread than originally thought. Here, we report a whole and annotated genome sequence of ceti CRO350, type 27 strain isolated from bottlenose dolphin carcass found in Croatian part northern Adriatic Sea.