Contents Heat stress is a major factor contributing to low fertility of dairy cows with great economic impact in industry. Heat‐stressed usually have reduced nutrient intake, resulting higher degree negative energy balance ( NEB ). The aim this study was investigate the seasonal thermal effect on lipid metabolism, antioxidant activity and reproductive performance cows. Thirty‐two healthy heifers were included study. According ambient temperature, animals divided into two groups: winter (N =...

Superoxide dismutase (SOD), total antioxidant capacity (TAC), and thiobarbituric acid reactive substances (TBARS) in seminal plasma were evaluated on the basis of receiver operating characteristics (ROC) analysis as predictors for distinguishing satisfactory from unsatisfactory boar semen samples after storage. SOD day 0 correlated significantly with progressive motility (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"...

Growing evidence indicates that macro- and microelements in the seminal plasma of humans various domestic animals are great importance due to their roles sperm metabolism, function, survival oxidative stress. In present study, we therefore determined concentrations fresh boar relation quality parameters after 3 days liquid storage was assessed. Twenty ejaculates from eight boars were collected, semen volume, concentration, motility, morphology, tail membrane integrity, permeability,...

In humans, transmission of bacteria causing fatal sepsis in the neonates through mother's milk has been reported. dogs, it is believed that from canine are not primary cause neonatal infections. Staphylococcus pseudintermedius colonizing skin and mucocutaneous junctions adult dogs can act as an opportunistic pathogen. This bacterium was previously isolated and, although, its dam's to newborn puppies a suggested, this hypothesis confirmed. A 4.5-year-old healthy Boston terrier dam had...

Summary The resazurin reduction assay depends on the ability of metabolically active cells to reduce redox dye resorufin. In present study we applied and made a diagnostic evaluation spectrophotometric application assess colour change in butanol extracted evaluate boar semen quality. Forty‐one samples from various breeds were included study. absorption peaks for resorufin found be 610 575 nm, respectively. Absorbance at where minimum overlap two was observed, used further analysis. Spearman...

ABSTRACT Background Wide variation in fertility rates is observed when using frozen bull semen, even the bulls have met quality standards for semen production. Therefore, a simple and reliable test to assess freezing potential of based on analysis fresh or blood would be great value. Attention now turning assessment seminal plasma components such as proteins elements. In present study, concentrations macro- microelements serum their correlation with characteristics after thawing were...

A challenge in freezing semen for short and long-term availability is avoiding damage to intact spermatozoa caused by the process. Vitrification protocols provide better results through less manipulation of shorter time compared slow techniques. Our research was aimed at improving vitrification methods canine semen. Semen quality determined 20 ejaculates after collection. Each ejaculate divided into eight aliquots, each with a different extender. The control extender contained TRIS, citric...

In the 1950s, infectious bovine rhinotracheitis/infectious pustular vulvovaginitis (IBR/IPV) disease was clinically detected and documented in cattle for first time Slovenia. The herpes virus 1 (BoHV-1) confirmed several times from infected herds by isolation on cell cultures. To keep IC virus-free, high biosecurity measures were introduced. Before entering IC, all calves are serologically tested quarantined. Bulls Slovenian insemination centres (IC) have been negative IBR /IPV infection...

Contents Evaluation of fat to protein ratio (FPR) in milk was performed for the prediction calving conception interval (CC) dairy cows. Reproduction parameters 51 high yielding cows were calculated from farm recording data and record a regular control. Spearman rank correlation analysis used determine between reproductive herd record. In time 75–90 days postpartum highest found FPR CC ( r = 0.414, p &lt; 0.05). Receiver operating characteristics (ROC) evaluate distinguish with different CC....

Our aim was to examine the diagnostic potential of matrix metalloproteinases MMP-2 and MMP-9 for identifying osteoarthritis in horse. Horses were divided into two groups - a positive group consisting 28 horses with cartilage damage distal interphalangeal joint negative 17 control horses. Clinical examination included evaluation lameness, flexion test, nerve blocks, X-ray arthroscopy. detected synovial fluid using gelatin zymography. Monomers appeared not be specific since they also occurred...

The aim of this study was to address the question whether changes in boar semen quality after short-term storage could be predicted on basis standard parameters and TNF-α level determined day collection under commercial conditions. Progressive motility showed highest positive correlation with morphology 0 collection, progressive 3 (P < 0.05) a negative acrosome abnormalities 0.05). According area receiver operating characteristics (ROC) curves (AUCs), also used predicting days (AUC > 0.5; P...

To assess that metabolic status of spermatozoa could provide a useful tool for evaluation semen quality.The accuracy the spectrophotometric application resazurin reduction assay was assessed using receiver operating characteristic (ROC) analysis.Areas under ROC curves (AUC) motile sperm concentration and index (SI) (sperm multiplied by square root percentage motility normal morphology) were 0.922. The best discrimination between poor good samples according to SI achieved at cut-off point...

Abstract The effects of stress on processes in the body are becoming an increasingly relevant research subject. reproductive ability bovine animals largely depends these effects, whilst embryo transfer is being used as a reproduction method. In this study, we established differences implantation heifers that were treated (N=17) with selenium (Se) and vitamins AD 3 E, non-treated heifers. Upon transfer, took blood samples from both groups total antioxidant status (TAS) value to analyze...

One of the features apoptosis is externalization phosphatidylserine which could be used to remove apoptotic cells from semen preparations. Magnetic-activated cell sorting using annexin V-conjugated microbeads bind enhance quality. Twelve boar samples after 3 days liquid storage at 16­­–17 °C were subjected magnetic-activated sorting. Bound and unbound fractions control flow cytometry following staining spermatozoa with Annexin V conjugated Alexa Fluor 488 propidium iodide. Four...

The aim of this study was to investigate the production and reproduction ability Slovenian brown-breed dairy cows according their exposure negative energy balance (NEB). Body condition score (BCS), concentrations metabolites such as non esterified fatty acids (NEFA), beta hydroxy butyrate (BHB), cholesterol urea were measured on day 14 postpartum progesterone profile determined for each animal. period closely monitored from conception by ultrasound examination in 10 intervals. Reproductive...

Matrix metalloproteinases were detected in reproductive tissues and seminal plasma of various animal species. The aim this study was to determine for the first time presence gelatinases metalloproteases boar correlate results with semen indicators. Gelatin zymography used simultaneous identification measurement gelatinase enzyme activity associated their molecular weights. Several forms identified boars. Those that stimulated by CaCl 2 inhibited EDTA phenanthroline considered as...

The quality of animal feed is increasingly affected by weather conditions, high humidity, and damage to grains, which have led various mycotoxin-producing moulds. aim this study was determine the effects combination deepoxy-deoxynivalenol beta-zearalenol on development preimplantation bovine embryos, extent presence both mycotoxin metabolites affects in vitro cultured or whether effect toxins enhances embryotoxicity. Ovaries were transported from abattoir laboratory and, after maturation...

Copper is an essential trace element for animals, required body, bone and wool growth, pigmentation, nervous system, haemoglobin formation white blood cell function. The animal's nutrient supply can be assessed with the help of haematological biochemical tests. aim study was to determine relationship between copper serum concentration parameters in sheep. Sheep from two farms were included study. Blood samples taken sheep (40 per flock) twice a year, fall after end grazing period spring...

The effect of fat to protein ratio (FPR) in milk for the prediction calving conception interval (CC) dairy cows is evaluated using different biostatistics' methods. Spearman rank correlation coefficient, a non-parametric alternative Pearson coefficient was used determine between reproductive parameters herd and data record. In time 60 90 days postpartum highest found FRP CC (r=0.415, p<0.05). FPR diagnostically ROC (receiver operating characteristics) analysis which based on completed 2x2...

In Slovenia, the control of bovine viral diarrhoea virus (BVDV) infections started in 1994. Since 2014, a voluntary programme has been running according to national rules that prescribe conditions for recognising, acquiring, and maintaining BVDV-free status an individual herd. The principle is based on periodical laboratory testing preventive measures need be strictly implemented Between 2014 2020, total 348 herds were included BVDV antibody testing, 25.0% tested detected positive. To...

The objective was to determine optimal thresholds of blood variables that are associated with liver lipid and glycogen content in dairy cows. Liver specimens 15 Holstein cows were taken 2 weeks postpartum (PP) used for determination content, respectively. Blood samples collected weekly, starting from week antepartum (AP) up PP. Selected parameters body condition scoring (BCS) evaluated through receiver operating characteristic curve analysis as indication fat the liver. Postpartal low (mode...