Silvio Špičić

ORCID: 0000-0001-8317-5831
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Research Areas
  • Brucella: diagnosis, epidemiology, treatment
  • Mycobacterium research and diagnosis
  • Tuberculosis Research and Epidemiology
  • Leptospirosis research and findings
  • Animal Diversity and Health Studies
  • Microbial infections and disease research
  • Infectious Diseases and Mycology
  • Salmonella and Campylobacter epidemiology
  • Animal Disease Management and Epidemiology
  • Viral Infections and Vectors
  • Galectins and Cancer Biology
  • Vector-Borne Animal Diseases
  • Burkholderia infections and melioidosis
  • Zoonotic diseases and public health
  • Yersinia bacterium, plague, ectoparasites research
  • Veterinary medicine and infectious diseases
  • Bacillus and Francisella bacterial research
  • Vector-borne infectious diseases
  • Rabies epidemiology and control
  • Bacteriophages and microbial interactions
  • Identification and Quantification in Food
  • Milk Quality and Mastitis in Dairy Cows
  • Diagnosis and treatment of tuberculosis
  • Bartonella species infections research
  • Animal Virus Infections Studies

Croatian Veterinary Institute
2016-2025

Between the years 2000 and 2004, 93,107 sera from 1,997 pig herds in 11 regions of Croatia were tested for presence antibodies against brucellosis. Positive results observed 67 seven (mean individual prevalence: approximately 1%; herd 3.4%). The all but two infected farms reared outdoors thus almost certainly came into contact with wildlife. From 2003 to 424 sera, which randomly collected hunted wild boar (Sus scrofa), also shown have a mean seroprevalence 27.6%. Brucella was isolated 88 out...

10.20506/rst.28.3.1947 article EN Revue Scientifique et Technique de l OIE 2009-12-01

This review paper briefly overviews various methods used to detect and identify Salmonella, a common foodborne zoonotic pathogen in veterinary medicine. Salmonella bacteria is mainly found food products and, when ingested, causes severe gastrointestinal symptoms. Due the ongoing presence of pathogenic production systems, it poses serious public health threat. Therefore, constant need remains enhance identification detection capable identifying this preventing outbreaks. The conventional...

10.46419/vs.56.6.6 article EN Veterinarska stanica 2025-03-16

Brucellosis is a ubiquitous emergent bacterial zoonotic disease causing significant human morbidity in Bosnia and Herzegovina. So far, high rate of resistant Brucella has been found worldwide. This study prospectively analysed the rates resistance among melitensis strains isolated Herzegovina.This included 108 B. isolates from 209 patients diagnosed at five medical centres The profiles for 13 most commonly used antimicrobials were studied standard broth (BB) cation-adjusted Mueller-Hinton...

10.1016/j.jgar.2022.02.005 article EN cc-by-nc-nd Journal of Global Antimicrobial Resistance 2022-02-16

Ferrets are highly susceptible to a wide range of mycobacteria, mainly M. bovis, avium, and triplex. Therefore, ferrets pose risk transmission mycobacteriosis, especially zoonotically relevant tuberculosis. The aim this study was describe the findings xenopi mycobacteriosis in pet ferret emphasize its zoonotic potential. A had history weight loss, apathy, hyporexia, hair loss. Abdominal ultrasound revealed splenomegaly with two solid masses cystic lesions liver. Fine-needle aspiration...

10.3390/pathogens13040328 article EN cc-by Pathogens 2024-04-16

AimTo present the surveillance data on Brucella melitensis, B. suis, and ovis infection in cattle, sheep, goats, swine Croatia obtained 2008 by serological, bacteriological, molecular methods for diagnostics of brucellosis domestic animals.MethodsWe serologically tested 42 785 cattle serums, 22 686 sheep goat 28 520 serums using Rose Bengal test, complement fixation various immunosorbent assays. We also 10 173 ram blood samples test. Bacteriological examination was conducted 214 collected...

10.3325/cmj.2010.51.320 article EN cc-by-nc-nd Croatian Medical Journal 2010-08-01

During a five-year period (2000 to 2004) 74,342 pigs were tested by the intradermal tuberculin test in Croatia. Of them, 248 (0.33%) positive and 91 (0.12%) found be suspicious 7 out of 13 farms included study. Gross pathological changes characteristic tuberculosis observed tuberculin-positive and/or swine. Mycobacterium was isolated from lymph nodes 183 234 swine (78.2%). For better epidemiological understanding, isolates typed conventional methods, PCR hybridisation. The results show that...

10.1556/avet.55.2007.1.1 article EN Acta Veterinaria Hungarica 2007-02-07

<p><strong>Objective. </strong>The aim of this study was to evaluate an ongoing outbreak brucellosis in southern region Bosnia and Herzegovina (BIH) on the epidemiological, clinical molecular level.</p><p><strong>Patients methods. </strong>This included 19 patients affected by between 2015 2017, Trebiševo (BIH). Out patients, 16 were admitted treated at Department Infectious diseases University Clinical Hospital Mostar, while three ambulatory care...

10.5644/ama2006-124.214 article EN cc-by-nc Acta Medica Academica 2018-06-25

Rapid-growth mycobacteria were isolated from two cases of cow mastitis with similar clinical appearance and within a narrow time frame. Mycobacteria on blood esculine agar. The Gram stained, Ziehl-Nielsen stained tested for growth at 25°C, 37°C 42°C, iron uptake, Löwenstein-Jensen (LJ) agar without 5% NaCl, arylsulphatase (3 days), tween 80 hydrolysis, tellurite reduction, nitrate reductase niacin synthesis. Molecular identification was performed using the GenoType CM AS tests (Hain...

10.46419/vs.53.5.11 article EN Veterinarska stanica 2022-01-05

During the years 2001 and 2002 on seven localities in Croatiaa survey prevalence of brucellosis wild boar was carried out. The included 271 (52.7%) female 243 (47.3%) male animals between 7 months 4 age weighing from 14 to 135 kg. On that occasion 514 blood samples were serologically analysed. For serological analysis indirect enzyme immunoassay (iELISA), Rose Bengal test (RBT), complement fixation (CFT) slow agglutination (SAT) used. In all investigated positive reactions established. Most...

10.17221/5684-vetmed article EN cc-by-nc Veterinární Medicína 2004-04-30

In total the samples of blood and kidneys 379 small rodents 154 wild swine were analysed. The antibodies to different serovars leptospires determined in 12.7% rodents, most often species Mus musculus (34.4%), A. agrestis (14.8%), flavicolis (10.8%), C. glareolus (9.4%) sylvaticus (6.5%). Most frequent findings sv. pomona (27.1%), sejroe (20.8%), australis (14.6%), hardjo, saxkoebing, tarassovi, grippotyphosa, bataviae icterohaemorrhagiae also established. Seventeen (4.5%) isolates...

10.17221/5786-vetmed article EN cc-by-nc Veterinární Medicína 2003-11-30

Summary The aim of the study was to give an account epidemic abortions in sheep caused by Salmonella enterica ssp. serovar Abortusovis, which occurred Dalmatia, south Croatia, winter 2003–2004. Five flocks with rate abortion ranging from 22% 38% during last‐third gestation were examined. Abortusovis isolated 13 vaginal smears and two fetuses. Direct inoculation found be superior pre‐enrichment enrichment selective broth for isolation. isolates biochemically identified, characterized...

10.1111/j.1439-0450.2006.00959.x article EN Journal of Veterinary Medicine Series B 2006-07-26

Bacillus anthracis can infect both livestock and humans. The importance of the treatment disease in humans has been underscored by bioterrorism events 2001 United States. presence PA B/C genes (pX01 pX02 plasmids) as well susceptibility to several antimicrobial substances was determined 11 strains isolated during two recent epizooties anthrax which occurred Croatia 2002 among sheep 2006/2007 cattle. pX01 plasmid observed all examined strains, including vaccinal Sterne strains. However,...

10.17221/1570-vetmed article EN cc-by-nc Veterinární Medicína 2011-01-31

Abstract In March 2022, an outbreak of Q fever ( Coxiella burnetii ) with non‐occupational exposure was confirmed in a semi‐urban area Čavle, Croatia. Veterinary and human epidemiological investigations were conducted to identify the source implement appropriate control measures. Three farms settled next each other near homes first cases at end street. The closest farm less than 500 meters away. These contained 161 adult sheep goats. Among animal samples analysed, all 16 goats (100%) 24/50...

10.1111/zph.13022 article EN Zoonoses and Public Health 2022-12-29

Abstract Background Cats are the primary reservoirs of bacterium Bartonella henselae , main cause cat-scratch disease in humans. The vector is cat flea, Ctenocephalides felis . In southeastern Europe, data lacking on prevalence B. infection cats, strains involved and risk factors associated with infection. Methods Blood samples collected ethylenediaminetetraacetic acid-containing tubes from 189 domestic cats (156 pet 33 stray cats) Zagreb, capital city Croatia, 10 counties throughout Croatia...

10.1186/s13071-024-06117-8 article EN cc-by Parasites & Vectors 2024-02-01

Bacillus anthracis, the causative agent of anthrax disease, is a worldwide threat to livestock, wildlife and public health. It also considered one most important pathogens bioterrorism. Rapid reliable diagnosis administration antimicrobials are essential for effective treatment. In this study, we determined in vitro susceptibilities 40 isolates B. anthracis isolated Croatia over recent two decades 18 antimicrobials. Whole-genome sequencing was performed, bioinformatics tools were used...

10.3390/antibiotics13070639 article EN cc-by Antibiotics 2024-07-11
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