A5039749885- Viral gastroenteritis research and epidemiology
- Physics of Superconductivity and Magnetism
- Animal Virus Infections Studies
- Viral Infections and Immunology Research
- Hepatitis Viruses Studies and Epidemiology
- Superconducting Materials and Applications
- Rare-earth and actinide compounds
- Superconductivity in MgB2 and Alloys
- Magnetic and transport properties of perovskites and related materials
- Magnetic properties of thin films
- Advanced Condensed Matter Physics
- High-pressure geophysics and materials
- Veterinary medicine and infectious diseases
- Virus-based gene therapy research
- Surface and Thin Film Phenomena
- Particle accelerators and beam dynamics
- Animal Disease Management and Epidemiology
- Quantum, superfluid, helium dynamics
- Atomic and Subatomic Physics Research
- Congenital Anomalies and Fetal Surgery
- Material Properties and Applications
- Liver Disease and Transplantation
- Advanced Electrical Measurement Techniques
- Liver Diseases and Immunity
- Spacecraft and Cryogenic Technologies
Federal Institute for Risk Assessment
2009-2023
Freie Universität Berlin
2010-2012
Polish Academy of Sciences
1979-1995
Instytut Nauk Prawnych
1995
Institute for Structural Research
1966
Group A rotaviruses (RVAs) are an important cause of diarrhoeal illness in humans, as well mammalian and avian animal species. Previous sequence analyses indicated that RVAs related only distantly to RVAs. Here, the complete genomes RVA strain 03V0002E10 from turkey (Meleagris gallopavo) 10V0112H5 pheasant (Phasianus colchicus) were analysed using a combination 454 deep sequencing Sanger technologies. An adenine-rich insertion similar found chicken 02V0002G3, but considerably shorter, was 3'...
ABSTRACT Hepatitis E virus (HEV) is an increasingly recognized zoonotic pathogen. Transmission suspected to occur from infected pigs or wild boars humans through direct contact, environmental pathways, contaminated food. However, the physical and chemical stability of HEV largely unknown, because suitable cell culture methods for infectivity measurement are missing. Here, we developed a titration method using infection line A549/D3 with genotype 3 strain 47832c subsequent counting...
Hepatitis E virus (HEV) is a human pathogen with increasing importance. The lack of efficient cell culture systems hampers systematic studies on its replication cycle, neutralization and inactivation. Here, several lines were inoculated the HEV genotype 3c strain 47832c, previously isolated from chronically infected transplant patient. At 14 days after inoculation highest genome copy numbers found in A549 cells, followed by PLC/PRF/5 whereas HepG2/C3A, Huh-7 Lunet BLR MRC-5 cells only weakly...
Rotaviruses are a leading cause of viral acute gastroenteritis in humans and animals. They grouped according to gene composition antigenicity VP6. Whereas group A, B, C rotaviruses found animals, D have been exclusively detected birds. Despite their broad distribution among chickens, no nucleotide sequence data exist so far. Here, the first complete genome rotavirus (strain 05V0049) is presented, which was amplified using sequence-independent amplification strategies degenerate primers. Open...
The genetic diversity of rotavirus A (RVA) strains is facilitated in part by reassortment. Although this process genome segment exchange has been reported frequently among mammalian RVAs, it remained unknown if RVAs also could package segments from avian RVA strains. We generated a simian strain SA11 reassortant containing the VP4 gene chicken 02V0002G3. To achieve this, we transfected BSR5/T7 cells with T7 polymerase-driven VP4-encoding plasmid, infected temperature-sensitive mutant, and...
Frozen berries have been repeatedly identified as vehicles for norovirus (NoV) transmission causing large gastroenteritis outbreaks. However, virus detection in is often hampered by the presence of RT-PCR-inhibiting substances. Here, several extraction methods subsequent real-time RT-PCR-based NoV-RNA strawberries were compared and optimized. NoV recovery rates (RRs) between 0.21 ± 0.13% 10.29 6.03% found when five different artificially contaminated strawberry batches analyzed ISO/TS15216-2...
Rotavirus A (RVA) is an important cause of diarrhea in humans and animals. However, RVA wild animals has only scarcely been investigated so far. Here, the presence ungulates hunted between 2019 2022 Brandenburg, Germany, was using real-time RT-PCR sequencing products. By analyzing intestinal contents, RVA-RNA detected 1.0% (2/197) boar (Sus scrofa), 1.3% (2/152) roe deer (Capreolus capreolus), 2.1% (2/95) fallow (Dama dama) but not 28 red (Cervus elaphus) samples. Genotyping identified...
In the last years, number of notified hepatitis E cases in humans has continuously increased Europe. Foodborne infection with zoonotic virus (HEV) genotype 3 is considered major cause this disease. Undercooked liver and raw sausages containing pigs wild boar are at high risk HEV. However, so far, no standardized method for detection HEV-RNA pig available.An international collaborative study on reproducibility involving 11 laboratories was performed an method, which consists steps sample...