ABSTRACT Hepatitis E virus (HEV) is an increasingly recognized zoonotic pathogen. Transmission suspected to occur from infected pigs or wild boars humans through direct contact, environmental pathways, contaminated food. However, the physical and chemical stability of HEV largely unknown, because suitable cell culture methods for infectivity measurement are missing. Here, we developed a titration method using infection line A549/D3 with genotype 3 strain 47832c subsequent counting...

Abstract Background Hepatitis E virus (HEV) is a pathogen of emerging concern in industrialized countries. The consumption wild boar meat has been identified as one risk factor for autochthonous HEV infections. Only limited information available about thermal stability HEV, mainly due to the lack rapid and efficient cell culture systems measurement infectivity. Methods A molecular biological method was implemented order distinguish disassembled from intact viral particles using RNase...

Collaboration across sectors, disciplines and countries is a key concept to achieve the overarching One Health (OH) objective for better human, animal environmental health. Differences in terminology interpretation of terms are still significant hurdle cross-sectoral information exchange collaboration within area OH including Surveillance (OHS). The development here described glossary collaborative effort three projects funded European Joint Programme (OHEJP). We describe infrastructure...

Abstract To examine the influence of two different probiotic bacteria on humoral immune system swine, animal studies were carried out with sows and their litters. The sows' feed was supplemented either Enterococcus faecium NCIMB 10415 (SF68) or Bacillus cereus var. toyoi 40112 beginning early in pregnancy. total IgA content faeces as well IgG concentration blood recorded before after weaning. same parameters determined piglets. In sows, only supplementation B. led to a clear increase faecal...

ABSTRACT Our understanding of the composition Escherichia coli populations in wild boars is very limited. In order to obtain insight into E. microflora boars, we studied isolates from jejunums, ileums, and colons 21 hunted five geographic locations Germany. Ten per section were subjected clonal determination using pulsed-field gel electrophoresis. One representative isolate clone was further investigated for virulence traits, phylogenetic affiliation, antimicrobial susceptibility....

Summary Upon studying the transmission of Escherichia coli from a sow to five her piglets, we observed domination coliform flora in piglets by single E. clone, especially after weaning. This haemolytic cloneH1 did not harbour any virulence determinants typical for intestinal pathogenic isolates swine but had gene profile very similar extraintestinal (ExPEC), including genes coding P fimbriae and several iron acquisition systems, besides having an affiliation phylogenetic B2 group. Overall,...

FoodChain-Lab is modular open-source software for trace-back and trace-forward analysis in food-borne disease outbreak investigations. Development of has been driven by a need appropriate several food-related outbreaks Germany since 2011. The allows integrated data management, linkage, enrichment visualization as well interactive supply chain analyses. Identification possible sources or vehicles facilitated calculation tracing scores food-handling stations (companies persons) food products...

Food microbiology researchers, risk assessment agencies and food business operators rely heavily on the reuse of knowledge that is available as data, models tools. Unfortunately, such remains challenging, safety data sets, tools are usually only in platform-dependent or software-dependent formats rarely comply to Findability, Accessibility, Interoperability, Reusability principles. In recent years, Risk Assessment Modelling Knowledge Integration Platform (RAKIP) Initiative developed...

Transferring the knowledge of predictive microbiology into real world food manufacturing applications is still a major challenge for whole safety modelling community. To facilitate this process, strategy creating open, community driven and web-based microbial model repositories proposed. These collaborative resources could significantly improve transfer from research commercial governmental also increase efficiency, transparency usability models. demonstrate feasibility, models Salmonella in...

Predictive microbial modelling and quantitative microbiological risk assessment, two important complementary areas within the food safety community, are generating a variety of scientific knowledge (experimental data mathematical models) resources (databases software tools) for exploitation this knowledge. However, application reusability is still hampered as access to exchange information between databases tools currently difficult time consuming. To facilitate transparent consistent new...

In the last decades microbial food safety community has developed a variety of valuable knowledge (e.g., mathematical models and data) resources databases software tools) in areas quantitative risk assessment (QMRA) predictive microbiology. However, reusability this exchange information between are currently difficult time consuming. This problem increased over due to lack harmonized data format rules for annotation. It includes common understanding basic terms concepts describe annotate...

In the last decades a large number of models have been developed in quantitative microbial risk assessment (QMRA) and predictive microbiology (PM) domains. These were generated with different scripting languages (e.g. R, MATLAB), commercial tools @Risk) or even proprietary software FSSP, FDA-iRISK). The heterogeneity used to generate together lack harmonized model exchange format has made (re)-use existing simulation environments very difficult. adoption information called Food Safety...

The Spatiotemporal Epidemiologic Modeler (STEM) is an open source software project supported by the Eclipse Foundation and used a global community of researchers public health officials working to track and, when possible, control outbreaks infectious disease in human animal populations. STEM not model or tool designed for specific disease; it flexible, modular framework supporting exchange integration models, reusable plug-in components, denominator data, available worldwide at...

Foodborne disease outbreaks of recent years demonstrate that due to increasingly interconnected supply chains these type crisis situations have the potential affect thousands people, leading significant healthcare costs, loss revenue for food companies, and—in worst cases—death. When a outbreak is detected, identifying contaminated quickly vital minimize suffering and limit economic losses. Here we present likelihood-based approach has accelerate time needed identify possibly products, which...

Abstract The identification of tumor-associated T cell epitopes has contributed significantly to the understanding interrelationship tumor and immune system is instrumental in development therapeutic vaccines for treatment cancer. Most known have been identified with prediction algorithms that compute potential capacity a peptide bind HLA class I molecules. However, naturally expressed need not necessarily be strong binders. To overcome this limitation available we established strategy...

Although Enterococcus faecium is used as a probiotic feed supplement in animal production, feeding of the bacterium to piglets resulted more severe infection with Salmonella Typhimurium DT104 during challenge experiment. To enlighten mode action by which E. affected piglets' health, we investigated influence on development intestinal and circulating immune cells experiment S. DT104. minimise varying impacts maternal immunity course infection, only were implemented that descended from...