A5050072303- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Monoclonal and Polyclonal Antibodies Research
- vaccines and immunoinformatics approaches
- Immune Cell Function and Interaction
- CAR-T cell therapy research
- Cutaneous lymphoproliferative disorders research
- Research on Leishmaniasis Studies
- RNA Interference and Gene Delivery
- Chemical Synthesis and Analysis
- Lymphoma Diagnosis and Treatment
- Trypanosoma species research and implications
- Cancer Immunotherapy and Biomarkers
- Fungal Infections and Studies
- Transgenic Plants and Applications
- Cancer, Hypoxia, and Metabolism
- Toxin Mechanisms and Immunotoxins
- Glycosylation and Glycoproteins Research
- Peptidase Inhibition and Analysis
- Advanced Biosensing Techniques and Applications
- Chronic Lymphocytic Leukemia Research
- Immune Response and Inflammation
- Cancer Research and Treatments
- Advanced Proteomics Techniques and Applications
- Chemokine receptors and signaling
Charité - Universitätsmedizin Berlin
2012-2024
Humboldt-Universität zu Berlin
2004-2019
Freie Universität Berlin
2013-2019
John Wiley & Sons (United Kingdom)
2019
Hudson Institute
2019
Humboldt State University
1998-2012
Technion – Israel Institute of Technology
2004
Connecticut Children's Medical Center
2004
TH Bingen University of Applied Sciences
2001
University of Tübingen
2001
Abstract T lymphocytes recognize their antigen as peptides associated with major histocompatibility complex (MHC) molecules. Peptides naturally presented by MHC class I molecules are uniform in length and have a specific motif, both defined the respective allele (Falk, K. et al. Nature 1991. 351: 290). These allele‐specific motifs should allow exact prediction of natural cell epitopes. H‐2K b ‐restricted epitopes, for example, eight amino acid residues conserved anchor at positions 5 8....
Abstract Alloreactive T lymphocytes are readily detected in unprimed animals although they have never encountered the alloantigen before. This well-established phenomenon is usually explained with assumption that a self-MHC molecule complexed defined peptide resembles allo-MHC another and induces corresponding cell specificities. Here, for first time support of this hypothesis, self-MHC-restricted peptides described clone was induced allo-MHC. The allo-MHC-specific CTL 2C derived from H-2b...
Synthetic oligodeoxynucleotides (ODNs), such as PF-3512676, that contain unmethylated cytosine-guanine motifs (CpG ODN) have been identified highly potent immune activators by in vitro examinations and murine models. CpG ODNs induce innate adaptive responses triggering Toll-like receptor 9 expressed human B cells plasmacytoid dendritic cells. A phase 1 study was initiated to investigate safety, tolerability, serum cytokine levels, cellular responses, clinical activity of intralesional...
Induction of cytotoxic CD8 T-cell responses is enhanced by the exclusive presentation antigen through dendritic cells, and innate stimuli, such as toll-like receptor ligands. On basis these 2 principles, we designed a vaccine against melanoma. Specifically, linked melanoma-specific Melan-A/Mart-1 peptide to virus-like nanoparticles loaded with A-type CpG, ligand for 9. was cross-presented, shown in vitro human cells HLA-A2 transgenic mice. A phase I/II study stage II-IV melanoma patients...
We previously isolated from mouse spleen an octapeptide (LSPFPFDL) that in association with the class I major histocompatibility complex protein Ld is recognized by antigen-specific receptor of alloreactive CD8+ T-cell clone (2C). Guided assay dependent upon same 2C receptor, we have now source another naturally occurring peptide. The second peptide (VAITRIEQLSPFPFDL) includes entire sequence and preliminary evidence suggests it may be a natural precursor octapeptide. On finding extensive...
Diagnostic tests for visceral leishmaniasis that are based on antigens of a single Leishmania strain can have low diagnostic performance in regions where heterologous parasites predominate. The aim this study was to investigate and compare the five serological tests, different antigens, three endemic countries leishmaniasis. A total number 231 sera symptomatic asymptomatic cases controls from East Sudan, North India South France were evaluated by following tests: rKLO8- rK39 ELISA, DAT...
Hybrid cell vaccination is a new cancer immune therapy approach that aims at recruiting T help for the induction of tumour specific cytolytic immunity. The vaccines are generated by fusion patients' cells with allogeneic MHC class II bearing to combine tumour's antigenicity immunogenicity molecules. Safety and anti-tumour activity this treatment were assessed in clinical trial has yielded one complete partial remission, 5 cases stable disease among 16 patients advanced stage metastatic...
Complex synthetic peptide libraries with defined amino acids in one or more positions of the H-2Kb-restricted cytotoxic T lymphocyte (CTL) epitopes SIINFEKL and RGYVYQGL mixtures 19 remaining were used to analyze structural requirements binding MHC class I molecules antigen recognition by CTLs. This approach provides means assess semiquantitatively contribution every acid peptides major histocompatibility complex (MHC) without biases introduced naturally processed peptides. Primary secondary...
Cytotoxic T lymphocytes (CTLs) have been shown to be relatively resistant cytolytic attack by other CTLs. We show here, however, that cloned CTLs, in the absence of cells, are destroyed exposure their cognate peptides (defined as those association with major histocompatibility complex proteins recognized antigen-specific receptor cell). Destruction is proportional peptide concentration and can prevented a second competes for presentation class I The speed extent peptide-induced changes...
Therapeutic vaccines, when used alone or in combination therapy with antileishmanial drugs, may have an important place the control of a variety forms human leishmaniasis. Here, we describe development adenovirus-based vaccine (Ad5-KH) comprising synthetic haspb gene linked to kmp11 via viral 2A sequence. In nonvaccinated Leishmania donovani–infected BALB/c mice, HASPB- and KMP11-specific CD8+ T cell responses were undetectable, although IgG1 IgG2a antibodies evident. After therapeutic...
A DNA vaccine for the induction of cell-mediated immunity against different types leishmaniasis was developed and successfully tested in human cell culture systems immunogenicity an animal model prophylaxis.
Visceral leishmaniasis is a protozoal disease caused by the intracellular parasites Leishmania donovani and L. chagasi/infantum, it usually deadly if not treated. To date, no vaccine exists for prophylaxis or immunotherapy, nor has been established which effector mechanisms of immune system are most instrumental against parasites. Recent reports have suggested that CD8+ T cells, in addition to CD4+ might play major roles defense infection cure disease. identify epitopes recognized cells can...
Human alpha-1-antitrypsin (A1AT) is a protease inhibitor that involved in the protection of lungs from neutrophil elastase enzyme drastically modifies tissue functioning. The glycoprotein consists 394 amino acids and N-glycosylated at Asn-46, Asn-83, Asn-247. A1AT deficiency currently treated with purified human serum. In view therapeutic applications, rA1AT was produced using novel neuronal cell line (AGE1.HN®) we investigated N-glycosylation pattern as well vitro anti-inflammatory activity...
Cytotoxic T lymphocytes are major effector cells in response to viral infections and allograft rejection implicated many other immunological reactions. Efficient induction of cytotoxic activity these but not all cases depends upon helper antigen-presenting so that at least three different cell types have work together. Here we present an vitro model for the cell-dependent which allows investigation collaboration cells. First results demonstrate linkage killer epitopes on surface one is a...
2C is a typical alloreactive cytotoxic T lymphocyte clone that recognizes two different ligands. These ligands are adducts of the allo-major histocompatibility complex (MHC) molecule H-2L d and an endogenous octapeptide, self-MHC H-2K b another peptide. MHC-binding T-cell assays with synthetic peptides in combination molecular modeling studies were employed to analyze structural basis for this crossreactivity. The surfaces complexes differ greatly densities distributions positive negative...
In the absence of other cells, cloned CTL in culture can undergo massive destruction upon addition a peptide that is recognized, association with CTL's class I MHC proteins, by Ag-specific TCR. To determine whether result individual recognition via its own TCR peptide-MHC-I complexes on surface ("suicide"), or to cytolytic attack some others same ("fratricide"), we compared rate peptide-induced cell death conventional cultures, where are free establish cell-cell contacts, cultures which were...
gp96 is a 96-kDa glycoprotein of the endoplasmic reticulum that believed to be involved in antigen processing as an intermediate carrier peptides for presentation by major histocompatibility complex (MHC) class I molecules. This function implies carries large array different represent antigenicity cell and can serve all MHC So far, evidence regarding these largely indirect based on experiments where mice immunized with from tumor or virus-infected cells developed T cellular immune responses...
Background Leishmaniasis defines a cluster of protozoal diseases with diverse clinical manifestations. The visceral form caused by Leishmania donovani is the most severe. So far, no vaccines exist for leishmaniasis despite indications naturally developing immunity, and sensitive immunodiagnostics are still at early stages development. Methodology/Principle Findings Establishing proteome-serological methodology, we mapped antigenicity parasites specificities immune responses in human...