One hundred thirty-six isolates, 88 human and 48 environmental, that met the requirements to belong genus Paenibacillus were identified using a polyphasic taxonomic approach known as 16S rRNA plus phenotypic traits. Thirty-seven species identified; some had not been previously reported from clinical samples. The main P. pabuli (13 isolates), provencensis (11), phoenicis (9) lautus (8). (11/13) (8/11) mainly environmental while (9/9) (6/8) isolates. Despite difficulties in assigning isolates...

The aims of this study were to explore the clinical distribution, by species, genus Nocardia and assess antimicrobial susceptibilities 10 most prevalent species identified in Spain.Over a year period (2005-14), 1119 strains molecularly subjected Etest. distribution resistance trends over sub-periods 2005-09 2010-14 also examined.Of examined, 82.9% belonged following species: cyriacigeorgica (25.3%), nova (15.0%), abscessus (12.7%), farcinica (11.4%), carnea (4.3%), brasiliensis (3.5%),...

Acinetobacter baumannii is one of the major pathogens involved in nosocomial outbreaks. The clonal diversity 729 epidemic strains isolated from 19 Spanish hospitals (mainly intensive care units) was analyzed over an 11-year period. Pulsed-field gel electrophoresis (PFGE) identified 58 PFGE types that were subjected to susceptibility testing, rpoB gene sequencing, and multilocus sequence typing (MLST). All multidrug resistant; colistin only agent which all susceptible. grouped into 16 clones...

ABSTRACT The levels of genetic relatedness 139 Stenotrophomonas maltophilia strains recovered from 105 hospitalized non-cystic fibrosis patients (51% medical wards, 35% intensive care units, and 14% surgical wards) 7 environmental sources in the same hospital setting during a 4-year period were typed by pulsed-field gel electrophoresis (PFGE) technique. A total 99 well-defined distinct Xba I PFGE patterns identified (Simpson's discrimination index, 0.996). dendrogram showed Dice similarity...

ObjectivesTo study the distribution, diversity and activity of Acinetobacter-derived cephalosporinase (ADC)-, carbapenem-hydrolysing oxacillinase (CHO)- metallo-β-lactamase (MBL)-encoding genes, most common insertion sequences (ISs), in genome nosocomial, epidemic, multidrug-resistant Acinetobacter baumannii (MDRAB) clones from Spain.

Abstract Background Group A Streptococcus (GAS) causes human diseases ranging in severity from uncomplicated pharyngitis to life-threatening necrotizing fasciitis and shows high rates of macrolide resistance several countries. Our goal is identify antimicrobial Spanish GAS isolates collected between 1994 2006 determine the molecular epidemiology ( emm /T typing PFGE) mechanisms those resistant erythromycin tetracycline. Results Two hundred ninety-five out 898 (32.8%) were resistant, with...

This work reports on antimicrobial resistance data for invasive Streptococcus pyogenes in Spain, collected by the 'Surveillance Program Invasive Group A Streptococcus', 2007-2020.emm typing was determined sequencing. Susceptibility to penicillin, tetracycline, erythromycin, and clindamycin via E-test. tetM, tetO, msrD, mefA, ermB, ermTR, ermT were sought PCR. Macrolide-resistant phenotypes (M, cMLSB, iMLSB) detected using erythromycin-clindamycin double-disk test. Resistant clones identified...

ABSTRACT Susceptibility to 41 antimicrobials was studied with 99 Stenotrophomonas maltophilia strains, and different pulsed-field gel electrophoresis profiles were identified among 130 prospectively collected isolates. Moxalactam, doxycycline, minocycline, clinafloxacin displayed the highest activity (≥98% susceptibility). Ticarcillin resistance (75%) reverted by clavulanate in 25% of strains. Trimethoprim-sulfamethoxazole 26.2% (≥4 [trimethoprim]/76 [sulfamethoxazole] μg/ml) dropped 11.1%...

Mutation frequencies were studied in 174 Stenotrophomonas maltophilia isolates from clinical and nonclinical environments by detecting spontaneous rifampin-resistant mutants otherwise-susceptible populations. The distribution of mutation followed a pattern similar to that found for other bacterial species, with modal value 1 x 10(-8). Nevertheless, the proportion showing below (hypomutators) was significantly higher S. than those so far reported organisms. Low particularly frequent among...

A large clonal outbreak of multidrug-resistant CC17 ST17 Enterococcus faecium containing Tn5382 in a hospital the north Spain is described.We characterized vancomycin-resistant E. isolates from 10 infected and 40 colonized inpatients single by PFGE, multiple-locus variable-number tandem-repeat analysis (MLVA) multilocus sequence typing (MLST). Genes encoding antibiotic resistance (ampicillin, aminoglycosides, macrolides, quinupristin/dalfopristin, quinolones, tetracycline) putative virulence...

To investigate the molecular mechanisms involved in high-level erythromycin resistance of clinical Spanish Campylobacter jejuni and coli strains. Overall susceptibilities 678 C. 119 strains, collected from 10 provinces during 2006 2007, were determined by Etest. In erythromycin-resistant determinants studied. The analysis was focused on region V 23S rRNA gene, rplD rplV ribosomal genes, regulatory CmeABC efflux pump. global rate to 3.8%. Among resistant 93% 7% jejuni. A2075G mutation gene...

Nosocomial outbreaks of multidrug-resistant Acinetobacter baumannii are worldwide concern. Using pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), and multiple locus variable number tandem repeat (VNTR) analysis (MLVA), the present work examines genetic diversity endemic epidemic A. clones isolated in a single hospital over twelve-year period.PFGE 405 baumannii-calcoaceticus complex isolates detected 15 endemic/epidemic PFGE types (EE1 to EE15) that grouped into...

ABSTRACT This study compares the identification, typing, and phylogenetic relationships of most prevalent clinical Nocardia species in Spain, as determined via sequence analysis their housekeeping genes gyrB rpoB , with results returned by gold standard 16S rRNA method. analyses identified abscessus N. cyriacigeorgica farcinica nova complex, that together account for more than half human nocardiosis cases recorded Spain. The individual discriminatory power respect to intraspecies calculated...

Our objective was to improve current knowledge of sporadic (Spo) nosocomial Acinetobactercalcoaceticus-Acinetobacter baumannii (Acb) complex populations, and thus better understand the epidemiology Spo endemoepidemic (EE) strains. Between 1999 2010, 133 isolates Acb were obtained from a single hospital. Species identified by gyrB-PCR, via gyrB- rpoB-sequencing. Clonal analysis undertaken using pulsed-field gel electrophoresis (PFGE) multilocus sequence typing. Susceptibility antimicrobial...

During 1991 to 1998 at least one Stenotrophomonas maltophilia pulmonary infection was observed in 25 (24%) of 104 cystic fibrosis patients the same unit our hospital Spain. Ribotyping and pulse-field gel electrophoresis (PFGE) characterization 76 S. isolates from these indicated an overall clonal incidence 47.1%, reflecting new strains 44% with repeated positive cultures for maltophilia. Six episodes were persistently colonized (> or = 6 months) strain. bacterial counts higher (geometric...

The severe morbidity of human brucellosis is one the main reasons for using molecular typing in epidemiological surveillance this worldwide zoonosis. Multiple-locus variable-number repeat analysis (MLVA-16), hypervariable octameric oligonucleotide fingerprinting (HOOF-print), and differences single nucleotide polymorphisms (SNPs) (codons 1249 1309) DNA-dependent RNA polymerase beta subunit (rpoB) were used to type a Brucella melitensis population (108 strains) collected from throughout Spain...

To report a clonal outbreak of ST17 vancomycin-resistant Enterococcus faecium (VREfm) carrying Tn1546 (vanA) in haemo-oncology ward tertiary teaching hospital the south Spain (January–September 2009). Twenty-two VREfm strains from 13 patients were characterized by PFGE, multiple-locus variable-number tandem-repeat analysis (MLVA) and multilocus sequence typing (MLST). Genes encoding antibiotic resistance putative virulence traits backbone investigated PCR. Plasmid characterization included...

ABSTRACT The quinolone resistance-determining regions (QRDRs) of topoisomerase II and IV genes from Stenotrophomonas maltophilia ATCC 13637 were sequenced compared with the corresponding 32 unrelated S. clinical strains for which ciprofloxacin MICs ranged 0.1 to 64 μg/ml. GyrA (Leu-55 Gln-155, Escherichia coli numbering), GyrB (Met-391 Phe-513), ParC (Ile-34 Arg-124), ParE (Leu-396 Leu-567) fragments strain showed high degrees identity phytopathogen Xylella fastidiosa , ranging 85.0 93.5%....

Abstract The spread of infectious agents through the mail has concerned public health officials for 5 centuries. dissemination anthrax spores in US 2001 was a recent example. In 1901, two medical journals reported outbreaks smallpox presumably introduced by letters contaminated with variola viruses. stability and infectivity virus are reviewed from both historical (anecdotal) perspective modern virologic studies. Bubonic plague contagious disease that led to quarantines as early 14th century...