A5047430467- Systemic Lupus Erythematosus Research
- Cardiovascular Function and Risk Factors
- Multiple Sclerosis Research Studies
- Peripheral Neuropathies and Disorders
- Renal Diseases and Glomerulopathies
- Cell Adhesion Molecules Research
- X-ray Diffraction in Crystallography
- Monoclonal and Polyclonal Antibodies Research
- Renal Transplantation Outcomes and Treatments
- Polyomavirus and related diseases
- Antibiotic Resistance in Bacteria
- Crystallization and Solubility Studies
- Diabetes and associated disorders
- Heart Rate Variability and Autonomic Control
- Pharmaceutical and Antibiotic Environmental Impacts
- Anatomy and Medical Technology
- Antibiotics Pharmacokinetics and Efficacy
- Cardiac Imaging and Diagnostics
- Dialysis and Renal Disease Management
- Surgical Simulation and Training
- Blood Pressure and Hypertension Studies
- Phagocytosis and Immune Regulation
- Health and Lifestyle Studies
- Steroid Chemistry and Biochemistry
- Electrical and Bioimpedance Tomography
Universidad San Pablo CEU
2013-2023
Universidad San Pablo
2020-2021
Brigham and Women's Hospital
2020
University of California, San Francisco
2020
Broad Institute
2020
Hospital General Universitario De Valencia
2018
Universidad Nacional de La Plata
1981-2016
Centro de Investigaciones Cardiovasculares
1996-2016
Consejo Nacional de Investigaciones Científicas y Técnicas
2016
Hospital Universitario Ramón y Cajal
1995-2010
To analyse the prevalence and diversity of integrons in faecal Escherichia coli isolates from healthy humans Spain. One hundred E. were obtained Levine agar plates samples 100 during March to October 2007. Susceptibility 16 antimicrobial agents was determined by disc diffusion method. The presence characterization class 1, 2 3 integrons, as well other resistance genes, performed PCR DNA sequencing. Integrases associated with 1 and/or identified 29 (intI1 gene 26 isolates, intI2 isolate intI1...
Mutation frequencies were studied in 174 Stenotrophomonas maltophilia isolates from clinical and nonclinical environments by detecting spontaneous rifampin-resistant mutants otherwise-susceptible populations. The distribution of mutation followed a pattern similar to that found for other bacterial species, with modal value 1 x 10(-8). Nevertheless, the proportion showing below (hypomutators) was significantly higher S. than those so far reported organisms. Low particularly frequent among...
Silastic tubes are used as training material for performing microvascular anastomoses. However, silastic texture differs from that of actual blood vessels. In the present work, we evaluate use preserved rat arterial segments in One-centimeter-long were obtained femoral, carotid, and abdominal arteries, cold saline solution, frozen. Trainees performed anastomoses using aforementioned answered questions about texture, consistency, wall resistance to needle, comparing tubes. They also asked...
Antibodies to lipids are part of the first line defense against microorganisms and regulate pro/anti-inflammatory balance. Viruses modulate cellular lipid metabolism enhance their replication, some these metabolites proinflammatory. We hypothesized that antibodies would play a main role in SARS-CoV-2 thus, they also avoid hyperinflammation, problem severe condition patients.Serum samples from COVID-19 patients with mild course, control group were included. IgG IgM different...
Aims: The present study was attempted to determine whether LV midwall mechanics yielded different conclusions about systolic function than the assessment of endocardial by echocardiography in spontaneously hypertensive rats (SHR). Methods and results: Thirty-six (18 Wistar normotensive (W), 18 [SHR]) anesthetized were studied with two-dimensional directed M-mode echocardiogram analyze structure (LV diameter, left ventricular wall thickness mass [LVM]) (endocardial shortening [ES] [MS])....
To evaluate the value of serum immunoglobulin G (IgG) and M (IgM) antibodies reactive with phosphatidylcholine (PC) lactosylceramide (LC) as biomarkers in MS.We developed an ultrasensitive ELISA technique to analyze IgG IgM LC PC, which we used samples from 362 patients MS, 10 non-MS myelin diseases (Non-MSMYDs), 11 nonmyelin neurologic (Non-MYNDs), 80 controls. MS included clinically isolated syndrome (CIS, n = 17), relapsing-remitting (RRMS, 62), secondary progressive (SPMS, 50), primary...
Abstract The detection of intrathecal IgA synthesis (IAS) in multiple sclerosis (MS) could be underestimated. To assess it, we develop a highly sensitive assay based on isoelectric focusing (IEF). 151 MS patients and 53 controls with different neurological diseases were recruited. concentration was analyzed using newly developed house ELISA. oligoclonal bands to detect IAS determined by IEF. Most individuals showed an within normal range serum samples (90.69%) but 31.37% had below the...
ABSTRACT. Mycophenolate mofetil (MMF) is a new immunosuppressive drug whose active metabolite, mycophenolic acid (MPA), blocks the action of inosine monophosphate dehydrogenase, resulting in inhibition novo purine synthesis. Thus, MPA has an antiproliferative effect on T and B lymphocytes also inhibits glycosylation cell surface adhesion proteins involved cell-cell contact recruitment circulating leukocytes to sites tissue damage inflammation. In this study, MMF mercury model nephritis was...
A series of fourteen new asymmetrical 1,3-diketone derivatives have been synthesized and evaluated in the ABTS, FRAP DPPH assays as a chemotype with antioxidant drug-like properties. All compounds displayed low cytotoxicity comparison to curcumin against human neuroblastoma SH-SY5Y cell line. Among them, (3Z,5E)-6-(2,5-difluoro-4-hydroxy-phenyl)-1,1,1-trifluoro-4-hydroxyhexa-3,5-dien-2-one (6b) (3Z,5E)-6-(2,3-difluoro-4-hydroxy-phenyl)-1,1,1-trifluoro-4-hydroxyhexa-3,5-dien-2-one (7b)...
Introduction: Medical education should promote the development of skills and abilities that can be applied to real-world work performance. The aim this study is evaluate technical methodological knowledge, as well physician-patient communication skills, one most important transversal competencies a good physician acquire; all in reliable, accurate objective way. Methods: We present rubric specifically designed implemented for evaluation specific physiology practical sessions, during second...
Abstract Left ventricular (LV) morphological and functional characteristics in 9 women suffering from pregnancy‐induced hypertension (PIH) were studied by means of echocardiograms. In order to distinguish which changes depended on the pressure values result pregnancy, 10 nonpregnant control with no heart disease normal pregnant (NP) results each groups compared. To evaluate structure, left systodiastolic diameters wall thickness measured. The only statistically significant difference was...
Four distinct epitopes (A, B1, B2, and C) have been functionally defined on the human alpha4 integrin. In this study, two cross-reactive antihuman monoclonal antibodies (mAb) (HP2/1 HP2/4 specific for B1 respectively) were used to characterize rat VLA-4 subunit define similar functional in rodent species. It was found that B2 anti-alpha4 mAb completely block adhesion fibronectin, but inhibition of vascular cell molecule-1 (VCAM-1) with HP2/1 lower than mAb. also observed epitope induced...
Abstract. Cell adhesion through different molecules is a crucial event in the inflammatory response. Integrins can only bind and mediate cellular after their activation by specific stimuli. The state of β1 integrin be assessed group monoclonal antibodies (HUTS) that selectively recognize integrins active form. A similar activated epitope rat was defined using anti-human antibody HUTS-21, which recognizes an activation-dependent on chain. It found divalent cations Mn 2+ Hg were able to induce...
Abstract We developed an ELISA assay demonstrating the high prevalence of serum IgM to phosphatidylcholine (IgM-PC) in first stages multiple sclerosis (MS). aimed analyze role IgM-PC as a biomarker response treatment. Paired samples from 95 MS patients were obtained before (b.t) and after (a.t) treatment with disease modifying therapies. Patients classified non-responders or responders treatment, according classical criteria. Serum concentration was analyzed using our house assay. The level...