A5029175418- Multiple Sclerosis Research Studies
- Cytomegalovirus and herpesvirus research
- Polyomavirus and related diseases
- Herpesvirus Infections and Treatments
- Viral Infections and Immunology Research
- Systemic Lupus Erythematosus Research
- Peripheral Neuropathies and Disorders
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Viral-associated cancers and disorders
- Autoimmune and Inflammatory Disorders Research
- Parvovirus B19 Infection Studies
- Chromosomal and Genetic Variations
- Immune Cell Function and Interaction
- Cytokine Signaling Pathways and Interactions
- Reproductive System and Pregnancy
- Monoclonal and Polyclonal Antibodies Research
- Full-Duplex Wireless Communications
- T-cell and Retrovirus Studies
- Plant Virus Research Studies
- MicroRNA in disease regulation
- Amyotrophic Lateral Sclerosis Research
- Rheumatoid Arthritis Research and Therapies
- Systemic Sclerosis and Related Diseases
- Neuroinflammation and Neurodegeneration Mechanisms
Instituto de Investigación Sanitaria del Hospital Clínico San Carlos
2016-2025
Hospital Clínico San Carlos
2014-2025
Spanish Multiple Sclerosis Network
2013-2025
Universität Innsbruck
2024
Brigham and Women's Hospital
2020
University of California, San Francisco
2017-2020
Universidad San Pablo
2020
Broad Institute
2020
Instituto de Salud Carlos III
2017-2018
Consorci Institut D'Investigacions Biomediques August Pi I Sunyer
2017
The prognostic role of cerebrospinal fluid molecular biomarkers determined in early pathogenic stages multiple sclerosis has yet to be defined. In the present study, we aimed investigate value chitinase 3 like 1 (CHI3L1), neurofilament light chain, and oligoclonal bands for conversion clinically isolated syndrome 75 patients with radiologically syndrome. Cerebrospinal levels CHI3L1 chain were measured by enzyme-linked immunosorbent assay. Uni- multivariable Cox regression models including as...
To identify differences in the metabolomic profile serum of patients with multiple sclerosis (MS) compared to controls and biomarkers disease severity.We studied 2 cohorts MS: a retrospective longitudinal cohort 238 74 prospective 61 41 serial samples. Patients were stratified into active or stable based on years assessment accounting for presence clinical relapses changes disability measured Expanded Disability Status Scale (EDSS). Metabolomic profiling (lipids amino acids) was performed by...
Objective: To validate kappa free light chain (KFLC) and lambda (LFLC) indices as a diagnostic biomarker in multiple sclerosis (MS). Methods: We performed multicenter study including 745 patients from 18 centers (219 controls 526 clinically isolated syndrome (CIS)/MS patients) with known oligoclonal IgG band (OCB) status. KFLC LFLC were measured paired cerebrospinal fluid (CSF) serum samples. Gaussian mixture modeling was used to define cut-off for indexes. Results: The the index 6.6 (95%...
The value of serum neurofilament light chain (sNfL) levels for predicting long-term disability in patients with multiple sclerosis (MS) remains controversial.
Abstract The potential for combining serum neurofilament light chain (sNfL) and glial fibrillary acidic protein (sGFAP) levels to predict worsening disability in multiple sclerosis remains underexplored. We aimed investigate whether sNfL sGFAP values identify distinct subgroups of patients according the risk their response disease-modifying treatments (DMTs). This multicentre study, conducted across 13 European hospitals, spanned from 15 July 1994 18 August 2022, with follow-up until 26...
The envelope proteins syncytin-1 and pHERV-W from the Human Endogenous Retroviral family 'W' (HERV-W) have been identified as potential risk factors in multiple sclerosis (MS). This study aims to evaluate both humoral cell-mediated immune response antigenic peptides derived these across different clinical forms inflammatory phases of MS. Indirect enzyme-linked immunosorbent assay (ELISA) was employed measure immunoglobulin G (IgG) responses syncytin-1env 486-500 pHERV-Wenv 486-504 MS...
Background Multiple Sclerosis is an autoimmune disease more prevalent in women than men. Associated Retrovirus element (MSRV) a member of type-W endogenous retrovirus family (HERV-W), known to be associated MS. Most HERVs are unable replicate but MSRV expression with reverse-transcriptase activity MS would explain reported DNA copy number increase patients. A potential link between HERV-W copies on chromosome X and gender differential prevalence has been suggested. The present study...
<h3>Background</h3> Recent studies have focused on the relationship between human herpesvirus 6 (HHV-6) and multiple sclerosis (MS). <h3>Objective</h3> To analyze HHV-6 messenger RNA expression in patients with relapsing-remitting (RR) MS vs healthy blood donors (HBDs). <h3>Design</h3> One hundred fifty-four subjects were enrolled study: 105 RRMS (32 relapse) 49 HBDs. Total DNA extracted from serum samples, respectively, analyzed by quantitative real-time reverse...
Objective To analyze the possible role of human herpesvirus (HHVs) and endogenous retroviruses (HERVs) infection in multiple sclerosis (MS) pathogenesis. Methods A total 92 cerebrospinal fluid (CSF) samples were collected: 48 from MS patients at first clinically evident demyelinating event, 23 with other inflammatory neurological diseases (OINDs) 21 non-inflammatory (ONINDs). Total DNA RNA isolated, prevalences viral loads herpes simplex virus (HSV), varicella-zoster (VZV), Epstein–Barr...
ABSTRACT Human herpesvirus 6 (HHV-6) is a ubiquitous virus with which infections have been associated pathologies ranging from delayed bone marrow engraftment to variety of neurological diseases. The lack standardized assay that can be used detect and estimate HHV-6 DNA contents in various clinical specimens lead has led discordant results among investigators on the potential association To identify most reliable sensitive assays, an identical set 11 coded serum samples spiked quantities...
Objective To identify a biomarker distinguishing patients who, despite primary progressive multiple sclerosis (PPMS) clinical course, may nonetheless benefit from immune therapy. Methods The presence or absence of both immunoglobulin (Ig) G and IgM oligoclonal bands (OCB) was blindly examined in paired cerebrospinal fluid (CSF) serum samples large PPMS patient cohort, related to imaging evidence focal inflammatory disease activity. Results Using cross‐sectional serial sampling subgroup...
Progressive multifocal leukoencephalopathy (PML) is a serious side effect associated with natalizumab treatment in multiple sclerosis (MS). PML risk increases individuals seropositive for anti-John Cunningham virus (JC) antibodies, prolonged duration of treatment, and prior exposure to immunosuppressants. We explored whether the presence lipid-specific immunoglobulin M oligoclonal bands cerebrospinal fluid (CSF; IgM bands), recognized marker highly inflammatory MS, may identify better able...
Syncytin-1 is the envelope protein of human endogenous retrovirus W (HERV-W). It has been related to multiple sclerosis (MS) but its role in cellular immunity and pathogenic mechanism autoimmune context are not fully understood. We analyzed syncytin-1 levels peripheral blood mononuclear cells (PBMC) subsets from healthy donors, MS patients relapse or remission, with acute infections by flow cytometry. PBMC cultures were also prepared analyze expression kinetics. had higher than controls....
Objective: To evaluate the role of parvovirus B19 (B19), varicella zoster virus (VZV), and human herpes 6 (HHV-6) in aetiopathology giant cell arteritis (GCA). Methods: Temporal artery biopsy specimens from 57 patients with GCA 56 controls were investigated. DNA was obtained by biopsy, quantitative real time polymerase chain reaction assay performed to establish prevalence viral load B19, VZV, HHV-6. Amplification β-globin gene used as internal positive control. Results: ( a ) detected 31/57...
<b>Objective:</b> To determine whether the human herpes viruses, cytomegalovirus (CMV), Epstein-Barr virus (EBV), and herpesvirus 6 (HHV-6), are detectable in serum peripheral blood mononuclear cells (PBMCs) of patients with rheumatoid arthritis (RA). <b>Methods:</b> 133 PBMC samples (61 RA, 72 healthy donors) 136 (59 77 were analysed by quantitative real time polymerase chain reaction for DNA prevalence viral load HHV-6, EBV, CMV. <b>Results:</b> For significant differences found EBV (56%...
Background. This study was undertaken in order to investigate the possible relation of HHV-6 and EBV relapsing-remitting MS (RRMS). Materials methods. A one-year follow up performed analysing peripheral blood mononuclear cells serum samples 57 patients with RRMS healthy donors (HBD) by a quantitative real time PCR, detect EBV. Clinical data (starting age EDSS increase) were collected. Results. We did not find any statistically significant difference for between HBD. Regarding HHV-6: i) There...
Abstract Objective An alteration in the composition of intestinal microbiota has been observed patients with multiple sclerosis (pwMS) respect to healthy controls (HC). Microorganism‐derived metabolites such as short‐chain fatty acids (SCFA) have suggested play a role disease. Thus, analyze association SCFA clinical and radiological parameters disease those related inflammatory response immune system. Methods Multicentric observational retrospective cross‐sectional study. In addition 161...
Context: Human herpesvirus 6 (HHV-6) has been linked with multiple sclerosis (MS).Objectives: To determine HHV-6 viral load in patients MS, and to analyze separately its 2 variants, HHV-6A HHV-6B.Patients Methods: We analyzed 149 blood serum samples; 103 were from relapsingremitting MS (33 during an relapse 70 remission), 46 healthy donors.To whether the genome variants present, we DNA using quantitative real-time polymerase chain reaction, which a sensitivity of 1 copy.Results: found...
Objective To analyze the titers of IgG and IgM antibodies against human herpesvirus 6A/B (HHV-6A/B) in multiple sclerosis (MS) patients treated with different disease modified therapies (DMTs) along two-years follow-up. Methods We collected 2163 serum samples from 596 MS; for 301 MS a 2-years follow-up was performed. Serum 337 healthy controls were also analyzed. Anti-HHV-6A/B analyzed by ELISA (Panbio). Results found that 129/187 (69.0%) decrease anti-HHV-6A/B after DMTs free relapses...
Multiple Sclerosis (MS) is an autoimmune demyelinating disease that occurs more frequently in women than men. Associated Retrovirus (MSRV) a member of HERV-W, multicopy human endogenous retroviral family repeatedly implicated MS pathogenesis. MSRV envelope protein elevated the serum patients and induces inflammation demyelination but, spite this pathogenic potential, its exact genomic origin mechanism generation are unknown. A possible link between HERV-W copy on chromosome Xq22.3, contains...
Gut microbiota has been related to multiple sclerosis (MS) etiopathogenesis. Short-chain fatty acids (SCFA) are compounds derived from microbial metabolism that have a role in gut-brain axis.To analyse SCFA levels plasma of MS patients and healthy donors (HD), the possible link between these both clinical data immune cell populations.Ninety-five 54 HD were recruited. Patients selected according their score Expanded Disability Status Scale (EDSS) (49 EDSS ≤ 1.5, 46 ≥ 5.0). studied samples by...
Background We aimed to investigate the potential of serum biomarker levels predict disability progression in a multicentric real-world cohort patients with primary progressive multiple sclerosis (PPMS). Methods A total 141 PPMS from 18 European MS centres were included. Disability was investigated using change Expanded Status Scale (EDSS) score over three time intervals: baseline 2 years, 6 years and last follow-up. Serum neurofilament light chain (sNfL), glial fibrillar acidic protein...