Jens Kühle
A5045590592- Multiple Sclerosis Research Studies
- Amyotrophic Lateral Sclerosis Research
- Skin and Cellular Biology Research
- Polyomavirus and related diseases
- Peripheral Neuropathies and Disorders
- Parkinson's Disease Mechanisms and Treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- RNA regulation and disease
- Rheumatoid Arthritis Research and Therapies
- Advanced Neuroimaging Techniques and Applications
- Neurogenesis and neuroplasticity mechanisms
- Alzheimer's disease research and treatments
- Prion Diseases and Protein Misfolding
- Hereditary Neurological Disorders
- Systemic Sclerosis and Related Diseases
- Long-Term Effects of COVID-19
- Neurogenetic and Muscular Disorders Research
- Traumatic Brain Injury and Neurovascular Disturbances
- Immunotherapy and Immune Responses
- S100 Proteins and Annexins
- Autoimmune and Inflammatory Disorders Research
- Systemic Lupus Erythematosus Research
- Acute Lymphoblastic Leukemia research
- Mycobacterium research and diagnosis
- Monoclonal and Polyclonal Antibodies Research
University Hospital of Basel
2016-2025
University of Basel
2015-2025
University Medical Center Freiburg
2011-2025
University of Freiburg
2008-2024
Hospital Base
2008-2024
University College London
2009-2024
Institute of Neuroimmunology of the Slovak Academy of Sciences
2017-2024
Institute for Biomedicine
2024
Neurology, Inc
2002-2024
The University of Melbourne
2020-2023
Multiple sclerosis (MS) is a chronic inflammatory demyelinating disease of the central nervous system unknown etiology. We tested hypothesis that MS caused by Epstein-Barr virus (EBV) in cohort comprising more than 10 million young adults on active duty US military, 955 whom were diagnosed with during their period service. Risk increased 32-fold after infection EBV but was not other viruses, including similarly transmitted cytomegalovirus. Serum levels neurofilament light chain, biomarker...
Neurofilament light chains (NfL) are unique to neuronal cells, shed the cerebrospinal fluid (CSF), and detectable at low concentrations in peripheral blood. Various diseases causing damage have resulted elevated CSF concentrations. We explored value of an ultrasensitive single-molecule array (Simoa) serum NfL (sNfL) assay multiple sclerosis (MS).sNfL levels were measured healthy controls (HC, n = 254) two independent MS cohorts: (1) cross-sectional with paired samples (n 142), (2)...
There is a long history of research into body fluid biomarkers in neurodegenerative and neuroinflammatory diseases. However, only few CSF are being used clinical practice. One the most critical factors biomarker inadequate powering studies because lack sufficient samples that can be obtained single-center studies. Therefore, collaboration between investigators needed to establish large biobanks well-defined samples. Standardized protocols for biobanking prerequisite ensure statistical power...
Abstract Background: Neuronal damage is the morphological substrate of persisting neurological disability. Neurofilaments (Nf) are specific cytoskeletal proteins neurons and their quantification has shown encouraging results as a biomarker for axonal injury. Methods: We aimed at comparing widely used conventional ELISA Nf light chain (NfL) with an electrochemiluminescence-based method (ECL assay) newly developed single-molecule array (Simoa) in clinically relevant cerebrospinal fluid (CSF)...
Neurofilament light protein (NfL) is elevated in cerebrospinal fluid (CSF) of a number neurological conditions compared with healthy controls (HC) and candidate biomarker for neuroaxonal damage. The influence age sex largely unknown, levels across disorders have not been systematically to date.
To test blood and CSF neurofilament light chain (NfL) levels in relation to disease progression survival amyotrophic lateral sclerosis (ALS).
Abstract Neurofilament light (NfL) protein is a marker of neuro-axonal damage and can be measured not only in cerebrospinal fluid but also serum, which allows for repeated assessments. There still limited knowledge regarding the association serum NfL (sNfL) with age subclinical morphologic brain changes their dynamics normal population. We sNfL by single molecule array (Simoa) assay 335 individuals participating population-based cohort study after mean follow-up time 5.9 years (n = 103)....
ObjectiveNeuronal damage is the morphological substrate of persisting neurological disability. Neurofilaments (Nf) are cytoskeletal proteins neurons and their release into cerebrospinal fluid has shown encouraging results as a biomarker for neurodegeneration. This study aimed to validate quantification Nf light chain (NfL) in blood samples, biofluid source easily accessible longitudinal studies. MethodsWe developed applied highly sensitive electrochemiluminescence (ECL) based immunoassay NfL...
<h3>Objective</h3> To assess the value of blood neurofilament light chain (NfL) as a biomarker recent, ongoing, and future disease activity tissue damage its utility to monitor treatment response in relapsing-remitting multiple sclerosis. <h3>Methods</h3> We measured NfL samples from 589 patients with sclerosis (from phase 3 studies fingolimod vs placebo, FREEDOMS interferon [IFN]-β-1a, TRANSFORMS) 35 healthy controls compared levels clinical MRI-related outcomes. <h3>Results</h3> At...
Neuro-axonal injury is a key factor in the development of permanent disability multiple sclerosis. Neurofilament light chain peripheral blood has recently emerged as biofluid marker reflecting neuro-axonal damage this disease. We aimed at comparing serum neurofilament levels sclerosis and healthy controls, to determine their association with measures disease activity ability predict future clinical worsening well brain spinal cord volume loss. was measured by single molecule array assay 2183...
The oral immunomodulator FTY720 has shown efficacy in patients with relapsing multiple sclerosis (MS). functionally antagonizes sphingosine 1-phosphate receptor-1 (S1P1) on T cells and consequently inhibits S1P/S1P1-dependent lymphocyte egress from secondary lymphoid organs. Little is known about the phenotype function of remaining peripheral blood during long-term treatment.T FTY720-treated, interferon-beta (IFNbeta)-treated untreated MS, healthy donors (HD) were analyzed respect to cell...
Cerebrospinal fluid collection by lumbar puncture (LP) is performed in the diagnostic workup of several neurological brain diseases. Reluctance to perform procedure among others due a lack standards and guidelines minimize risk complications, such as post-LP headache or back pain.We provide consensus for LP complications. The recommendations are based on (1) data from large multicenter feasibility study (evidence level II-2), (2) systematic literature review needle characteristics...
Background and objective: We explored which clinical biochemical variables predict conversion from clinically isolated syndrome (CIS) to definite multiple sclerosis (CDMS) in a large international cohort. Methods: Thirty-three centres provided serum samples 1047 CIS cases with at least two years’ follow-up. Age, sex, presentation, T2-hyperintense lesions, cerebrospinal fluid (CSF) oligoclonal bands (OCBs), CSF IgG index, cell count, 25-hydroxyvitamin D3 (25-OH-D), cotinine titres against...
Abstract Objective To evaluate cerebrospinal fluid ( CSF ) and serum neurofilament light chain (NfL) levels in genetic frontotemporal dementia FTD as a potential biomarker the presymptomatic stage during conversion into symptomatic stage. Additionally, to correlate NfL clinical neuroimaging parameters. Methods In this multicenter case–control study, we investigated 174 subjects (48 controls, 40 carriers 86 patients with microtubule‐associated protein tau MAPT ), progranulin GRN chromosome 9...
Neurofilament light chain (NfL) levels in the cerebrospinal fluid (CSF) of multiple sclerosis (MS) patients correlate with degree neuronal injury. To date, little is known about NfL concentrations serum relapsing remitting (RRMS) and their relationship CSF magnetic resonance imaging (MRI) measures disease severity. We aimed to validate quantification samples RRMS, as a biofluid source easily accessible for longitudinal studies.A total 31 RRMS underwent sampling. After median time 3.6 years,...
Neurofilaments are elevated in the cerebrospinal fluid (CSF) and serum of amyotrophic lateral sclerosis (ALS) patients. However, timing this increase is unknown. To characterize premanifest disease phase, we performed a cross-sectional study on asymptomatic (n = 12) symptomatic 64) ALS mutation carriers family controls 19). NF-L (neurofilament-light chain) pNF-H (phosphorylated neurofilament-heavy normal before symptom onset increased by at least an order magnitude early CSF (pNF-H) or...
<h3>Importance</h3> Unrecognized demyelinating events often precede the clinical onset of multiple sclerosis (MS). Identification these at time occurrence would have implications for early diagnosis and search causal factors disease. <h3>Objective</h3> To assess whether serum neurofilament light chain (sNfL) levels are elevated before MS onset. <h3>Design, Setting, Participants</h3> Nested case-control study among US military personnel who samples stored in Department Defense Serum...