One hundred thirty-six isolates, 88 human and 48 environmental, that met the requirements to belong genus Paenibacillus were identified using a polyphasic taxonomic approach known as 16S rRNA plus phenotypic traits. Thirty-seven species identified; some had not been previously reported from clinical samples. The main P. pabuli (13 isolates), provencensis (11), phoenicis (9) lautus (8). (11/13) (8/11) mainly environmental while (9/9) (6/8) isolates. Despite difficulties in assigning isolates...

The aims of this study were to explore the clinical distribution, by species, genus Nocardia and assess antimicrobial susceptibilities 10 most prevalent species identified in Spain.Over a year period (2005-14), 1119 strains molecularly subjected Etest. distribution resistance trends over sub-periods 2005-09 2010-14 also examined.Of examined, 82.9% belonged following species: cyriacigeorgica (25.3%), nova (15.0%), abscessus (12.7%), farcinica (11.4%), carnea (4.3%), brasiliensis (3.5%),...

ObjectivesTo study the distribution, diversity and activity of Acinetobacter-derived cephalosporinase (ADC)-, carbapenem-hydrolysing oxacillinase (CHO)- metallo-β-lactamase (MBL)-encoding genes, most common insertion sequences (ISs), in genome nosocomial, epidemic, multidrug-resistant Acinetobacter baumannii (MDRAB) clones from Spain.

This work reports on antimicrobial resistance data for invasive Streptococcus pyogenes in Spain, collected by the 'Surveillance Program Invasive Group A Streptococcus', 2007-2020.emm typing was determined sequencing. Susceptibility to penicillin, tetracycline, erythromycin, and clindamycin via E-test. tetM, tetO, msrD, mefA, ermB, ermTR, ermT were sought PCR. Macrolide-resistant phenotypes (M, cMLSB, iMLSB) detected using erythromycin-clindamycin double-disk test. Resistant clones identified...

ABSTRACT This study compares the identification, typing, and phylogenetic relationships of most prevalent clinical Nocardia species in Spain, as determined via sequence analysis their housekeeping genes gyrB rpoB , with results returned by gold standard 16S rRNA method. analyses identified abscessus N. cyriacigeorgica farcinica nova complex, that together account for more than half human nocardiosis cases recorded Spain. The individual discriminatory power respect to intraspecies calculated...

Our objective was to improve current knowledge of sporadic (Spo) nosocomial Acinetobactercalcoaceticus-Acinetobacter baumannii (Acb) complex populations, and thus better understand the epidemiology Spo endemoepidemic (EE) strains. Between 1999 2010, 133 isolates Acb were obtained from a single hospital. Species identified by gyrB-PCR, via gyrB- rpoB-sequencing. Clonal analysis undertaken using pulsed-field gel electrophoresis (PFGE) multilocus sequence typing. Susceptibility antimicrobial...

The severe morbidity of human brucellosis is one the main reasons for using molecular typing in epidemiological surveillance this worldwide zoonosis. Multiple-locus variable-number repeat analysis (MLVA-16), hypervariable octameric oligonucleotide fingerprinting (HOOF-print), and differences single nucleotide polymorphisms (SNPs) (codons 1249 1309) DNA-dependent RNA polymerase beta subunit (rpoB) were used to type a Brucella melitensis population (108 strains) collected from throughout Spain...

To report a clonal outbreak of ST17 vancomycin-resistant Enterococcus faecium (VREfm) carrying Tn1546 (vanA) in haemo-oncology ward tertiary teaching hospital the south Spain (January–September 2009). Twenty-two VREfm strains from 13 patients were characterized by PFGE, multiple-locus variable-number tandem-repeat analysis (MLVA) and multilocus sequence typing (MLST). Genes encoding antibiotic resistance putative virulence traits backbone investigated PCR. Plasmid characterization included...

The soil-borne pathogen Nocardia sp. causes severe cutaneous, pulmonary, and central nervous system infections. Against them, co-trimoxazole (SXT) constitutes the mainstay of antimicrobial therapy. However, some strains show resistance to SXT, but underlying genetic basis is unknown. We investigated presence determinants class 1-3 integrons in 76 SXT-resistant by PCR sequencing. By E test, these clinical showed SXT minimum inhibitory concentrations ≥32:608 mg/L (ratio 1:19 for trimethoprim:...

Nocardia species are difficult to identify, a consequence of the ever increasing number known and their homogeneous genetic characteristics. 16S rRNA analysis has been gold standard for identifying these organisms, but proteomic techniques such as matrix-assisted laser desorption ionization-time flight (MALDI-TOF MS) housekeeping gene analysis, have also explored. One hundred high (n=25), intermediate (n=20) low (n=55) prevalence (for Spain) strains belonging 30 were identified via MALDI-TOF...

The aim of this study was to determine, using molecular methods, whether rifampicin and fluoroquinolone resistance present in a clinical Brucella melitensis population. Sixty-two B. strains, isolated from humans—most experiencing their first brucellosis episode—over an 11 year period Spain, were genotyped by multiple locus variable analysis (MLVA-16) for future studies. In the work, screening undertaken detect presence rpoB gyrA/gyrB/parC/parE mutations (previously described vitro spp....

Botulism is a severe neuroparalytic disorder that can be potentially life-threatening. In Barcelona, Spain, no outbreaks had been reported in the past 25 years. However, September 2011, two occurred involving different families. A rare case of Clostridium baratii which produced neurotoxin F outbreak was detected five family members who shared lunch, and several days before another affected by C. botulinum toxin probably present homemade pâté.

Burkholderia cepacia complex (BCC) are non-fermenting Gram-negative bacteria that can chronically colonize the lungs of people with cystic fibrosis (pwCF), causing a severe and progressive respiratory failure, post-transplant complications epidemic outbreaks. Therefore, rapid accurate identification these is relevant for pwCF, in order to facilitate early eradication prevent chronic colonization. However, BCCs often quite difficult detect on culture media as they have slow growth rate be...

We describe a case of traumatic ocular endophthalmitis caused by Nocardia kruczakiae after vegetable trauma in an immunocompetent child. A 5-year-old boy suffered from with palm tree leaflet. Two months later, he was diagnosed infectious uveitis and intumescent cataract anterior capsule rupture. Intensive treatment systemic topical vancomycin, ceftazidime methylprednisolone began. After 1 month, underwent phacoemulsification intraocular lens implantation (IOL). some episodes reactivation,...

gyrB is used to improve the identification of Nocardia species N. brasiliensis, higoensis, ignorata, otitidiscaviarum, paucivorans, pneumoniae, puris, takedensis, veterana, and vinacea, but it does not another 12 studied species. provides typing phylogenetic markers for carnea, transvalensis, otitidiscaviarum.

Botulism is a low incidence but potentially fatal infectious disease caused by neurotoxins produced mainly Clostridium botulinum. There are different routes of acquisition, food-borne and infant/intestinal being the most frequent presentation, antitoxin treatment choice in all cases. In Spain, botulism under surveillance, case reporting mandatory.This retrospective study attempts to provide more complete picture epidemiology Spain from 1997 2019 an assessment treatment, including...

Nocardia species, one of the most predominant Actinobacteria soil microbiota, cause infection in humans following traumatic inoculation or inhalation. The identification, typing, phylogenetic relationship and antimicrobial susceptibilities 38 strains from Lara State, Venezuela, were studied by 16S rRNA gyrB (subunit B topoisomerase II) genes, multilocus sequence analysis (MLSA), whole-genome sequencing (WGS), microdilution. results compared with those for human strains. Just seven species...

Two hundred and eighty-six isolates from human clinical samples were identified between 1996 2019 as belonging to 8 families, 19 genera 88 species of Actinobacteria. The most Streptomyces (182 strains 45 species), Actinomadura (29 strains, 5 Nocardiopsis (21 6 species) Dietzia (18 species). rest the (15) contained 27 with 36 isolates. Of studied, only 13/88 had been documented previously samples, in some cases, true pathogens. In this sense, a literature review found infections or without...

We report the first case of surgical site infection caused by Shewanella putrefaciens. The occurred in a patient who underwent surgery for an incarcerated umbilical hernia. source was unclear, and clinical outcome good after treatment with ciprofloxacin. review literature shows that S. putrefaciens has been sporadically implicated skin soft tissue infections, especially patients poor personal hygiene, burns, or trauma injuries related water exposure. percentage bacteremia septicemia...

We report a case of cellulitis caused by Shewanella haliotis. The infection occurred on the leg in patient with multiple pathologies, including diabetes, liver cirrhosis, and chronic venous insufficiency. source was unclear, clinical outcome good after treatment ciprofloxacin for 14 days. review literature shows that S. haliotis has been rarely implicated human infections. Sporadic cases severe skin soft tissue infections, bacteremia, sepsis have reported from Asian countries. should be...

To determine whether the neurotoxin BoNT/B2 causing botulism in Spain is clonal, genetic diversity and phylogenetic relationships of Clostridium botulinum from food-borne episodes infant cases condition were explored. The toxin gene (bont) subtype, variable region flagellin (flaVR), a seven-gene multi-locus sequence type examined by sequencing 37 BoNT-positive cultures obtained over period 2010 to 2022. Out events, 16 associated with bont/b2. Eight bont/b2 alleles detected [nucleotide...

The immunosuppression conditions and the presence of medical devices in patients favor Gordonia infections. However, features this aerobic actinomycete have been little explored. Strains (n = 164) were characterized with 16S rDNA secA1 genes to define their phylogenetic relationships, subjected broth microdilution profile antimicrobial susceptibilities species that caused infections Spain during 2005-2021 period. Four out eleven identified responsible for 86.0% infections: sputi (53.0%),...