A5026217969- Listeria monocytogenes in Food Safety
- Essential Oils and Antimicrobial Activity
- Bacterial biofilms and quorum sensing
- Microbial Inactivation Methods
- Infant Nutrition and Health
- Bacterial Infections and Vaccines
- Escherichia coli research studies
- Malaria Research and Control
- Neuroinflammation and Neurodegeneration Mechanisms
- Bacterial Genetics and Biotechnology
- Vibrio bacteria research studies
- Mosquito-borne diseases and control
- Parasites and Host Interactions
- Antibiotic Resistance in Bacteria
- Food Allergy and Anaphylaxis Research
Institut Pasteur
2016-2024
Biology of Infection
2016-2024
Inserm
2020-2024
Université Paris Cité
2022-2024
Brigham and Women's Hospital
2013-2023
Harvard University
2022-2023
Sorbonne Université
2014
Centre d'Immunologie et des Maladies Infectieuses
2014
Significance It is thought antibiotic resistance carries a fitness cost and reduces microbial virulence. Using high-throughput sequencing analysis of transposon insertion bank in Pseudomonas aeruginosa , we found enhanced for vivo mucosal colonization systemic spread strains with insertions the oprD gene. This conferred to carbapenem antibiotics as well killing at acidic pH by normal human serum along increased cytotoxicity against murine macrophages. RNA-sequencing revealed that deficiency...
Experimental genetics have been widely used to explore the biology of malaria parasites. The rodent parasites Plasmodium berghei and less frequently P. yoelii are commonly utilised, as their complete life cycle can be reproduced in laboratory because they genetically tractable via homologous recombination. However, due limited number drug-selectable markers, multiple modifications parasite genome difficult achieve require large numbers mice. Here we describe a novel strategy that combines...
Worldwide, Escherichia coli is the leading cause of neonatal Gram-negative bacterial meningitis, but full understanding pathogenesis this disease not yet achieved. Moreover, to date, no vaccine available against meningitis.
Abstract Central nervous system infections are amongst the most severe 1,2 , yet mechanisms by which pathogens access brain remain poorly understood. The model microorganism Listeria monocytogenes (Lm) is a major foodborne pathogen that causes neurolisteriosis, one of deadliest central 3,4 . While immunosuppression well-established host risk factor for neurolisteriosis 3,5 little known regarding bacterial factors underlying Lm neuroinvasion. We have developed clinically-relevant experimental...
Abstract Background Worldwide, Escherichia coli is the leading cause of neonatal Gram-negative bacterial meningitis, but full understanding pathogenesis this disease not yet achieved. Moreover, to date, no vaccine available against meningitis. Methods Here, we used Transposon Sequencing saturated banks mutants (TnSeq) evaluate E. K1 genetic fitness in murine We identified genes encoding for factors important systemic dissemination and brain infection, focused on products with a likely...
Abstract Central nervous system infections are amongst the most severe, yet mechanisms by which pathogens access brain remain poorly understood. The model microorganism Listeria monocytogenes (Lm) is a major foodborne pathogen that causes neurolisteriosis, one of deadliest central infections. While immunosuppression well-established host risk factor for little known regarding bacterial factors underlying Lm neuroinvasion. We have developed clinically-relevant experimental using hypervirulent...