A5055169451- Plant biochemistry and biosynthesis
- Plant and fungal interactions
- Postharvest Quality and Shelf Life Management
- Oxidative Organic Chemistry Reactions
- Plant Physiology and Cultivation Studies
- Phytochemicals and Antioxidant Activities
- Synthetic Organic Chemistry Methods
- Asymmetric Synthesis and Catalysis
- Botanical Research and Chemistry
- Metabolomics and Mass Spectrometry Studies
- Horticultural and Viticultural Research
- Plant Gene Expression Analysis
- Inorganic and Organometallic Chemistry
- Plant Pathogens and Fungal Diseases
- Fermentation and Sensory Analysis
- Selenium in Biological Systems
- Gut microbiota and health
- Entomopathogenic Microorganisms in Pest Control
- Chemical Reaction Mechanisms
- Chemical synthesis and alkaloids
- Natural product bioactivities and synthesis
- Essential Oils and Antimicrobial Activity
- Genomics, phytochemicals, and oxidative stress
- Moringa oleifera research and applications
- Analytical Chemistry and Chromatography
Plant & Food Research
2009-2023
Palmerston North Hospital
1990-2019
AgResearch
1993-2010
University of Auckland
1995-2010
Massey University
1990-2006
The University of Sydney
1995-2001
Food Research Institute
1996
Université de Sherbrooke
1981
National Research Council Canada
1981
The biosynthesis of anthocyanin in many plants is affected by environmental conditions. In apple (Malus × domestica Borkh.), concentrations fruit anthocyanins are lower under hot climatic We examined the accumulation peel maturing 'Mondial Gala' and 'Royal apples, grown both temperate climates, using artificial heating on-tree fruit. Heat caused a dramatic reduction concentration transcripts genes biosynthetic pathway. Heating rapidly reduced expression R2R3 MYB transcription factor (MYB10)...
DOI: 10.1111/j.1469-8137.2009.02771.x Commentary p 1
Several isomeric compounds derived from the spiro systems 5 to 9 (Scheme 4) were obtained acid cyclization of appropriate dihydroxy ketone precursor.The configuration and conformation products was determined by 13 C nmr analysis equilibration studies. The experimental results can be rationalized taking into account anomeric exo-anomeric effects usual steric interactions.
Volatile organic compounds (volatiles) comprise a chemically diverse class of low molecular weight having an appreciable vapor pressure under ambient conditions. Volatiles produced by plants attract pollinators and seed dispersers, provide defense against pests pathogens. For insects, volatiles may act as pheromones directing social behavior or cues for finding hosts prey. humans, are important flavorants possible disease biomarkers. The marine environment is also major source halogenated...
Solid phase microextraction (SPME) was evaluated for use in the quantification of aroma volatile production by Granny Smith apples during cool storage. Particular attention paid to quantifying alpha-farnesene (3,7,11-trimethyldodeca-1,3(E),6(E),10-tetraene) due its involvement superficial scald, a disorder stored apples. Comparison between SPME and solid extraction (SPE) showed that fiber had greater adsorption high molecular weight (MW) volatiles such as alpha-farnesene. When sampling SPME,...
Biosynthesis of straight-chain ester volatiles by Granny Smith and Red Delicious apples was investigated using deuterium-labeled fatty acids, C-6 aldehydes, alcohols. Perdeuterated saturated monounsaturated acids were metabolized to hexyl-d11, hexanoate-d11, heptanoate-d13, octanoate-d15 esters, whereas perdeuterated linoleic acid produced only hexyl-d11 hexanoate-d11 esters. Exposure fruit vapors deuterated 3Z-hexenal, 2E-hexenal, hexanal identified the following biosynthetic processes: (1)...
D. Rowan, M. B. Hunt and L. Gaynor, J. Chem. Soc., Commun., 1986, 935 DOI: 10.1039/C39860000935
2-Methylbutanoate esters, especially ethyl 2-methylbutanoate, are key contributors to fruit aroma. The biosynthetic origins and interconversions of 2-methylbutyl 2-methylbutanoate esters in Red Delicious Granny Smith apples were determined by feeding deuterium-labeled substrates with GC−MS identification the deuterated aroma volatiles produced. Deuterium-labeled isoleucine was fed apple peel, 2-methylbutanoic-d3 acid, 2-methylbutanol-d3, 2-methylbutanoate-d3 as vapor whole apples. An array...
The 'fruity' attributes of ripe apples (Malus × domestica) arise from our perception a combination volatile ester compounds. Phenotypic variability in production was investigated using segregating population 'Royal Gala' (RG; high production) 'Granny Smith' (GS; low cross, as well transgenic RG plants which expression the alcohol acyl transferase 1 (AAT1) gene reduced. In GS population, 46 quantitative trait loci (QTLs) for esters and alcohols were identified on 15 linkage groups (LGs)....
Summary Consumers of whole foods, such as fruits, demand consistent high quality and seek varieties with enhanced health properties, convenience or novel taste. We have raised the polyphenolic content apple by genetic engineering anthocyanin pathway using transcription factor MYB 10 . These apples very concentrations foliar, flower fruit anthocyanins, especially in peel. Independent lines were examined for impacts on tree growth, photosynthesis characteristics. Fruit analysed changes...
A neglected group of highly unsaturated hydrocarbons was how the dendralenes were described 16 years ago in a review Angewandte Chemie. These compounds are no more! The hitherto unknown [5]-, [6]- (see scheme), and [8]dendralenes synthesized by thermolysis masked sulfolene derivatives which, turn, prepared Stille coupling. same approach offers an improved route to pure samples [3]- [4]dendralene, full spectroscopic details which reported for first time.
The sesquiterpene α-farnesene (1) and its conjugated triene oxidation products accumulate in the skin of apples after harvest are implicated as causal agents superficial scald. Conjugated analogues were synthesized applied to surface Granny Smith either vapors or squalane. Farnesyl hydroperoxide (2a), trienol (2b), endoperoxide (3a), dehydronerolidol (5), cumyl (4) all produced symptoms scald when at nanomolar doses. Scald-inducing activity was dependent on mode application. (2a) most active...
The biosynthesis of volatile esters by Red Delicious apples was investigated incubating fruit tissue with deuterated flavor precursors at various times after controlled atmosphere (CA) storage and measuring deuterium incorporation into branched-chain ester volatiles. 2-Methylbutyl acetate the only not significantly reduced CA storage. Conversion 2-methylbutanol to 2-methylbutyl 2-methylbutanoic acid ethyl 2-methylbutanoate hexyl limited availability substrates but acetyl-CoA, ethanol, or...
Crohn's disease is an inflammatory disorder of the bowel, believed to arise from dysregulation intestinal mucosal immunity. The interleukin-10-deficient (IL10-/-) mouse, which develops inflammation in presence gut microflora, serves as a mouse model disease. Nontargeted urinary metabolite profiling was carried out identify systemic metabolic changes associated with development caused by IL10-deficiency. Spot urine samples, collected IL10-/- and wildtype mice at ages 5.5, 7, 8.5, 10.5 weeks...
Interleukin-10 is an immunosuppressive cytokine involved in the regulation of gastrointestinal mucosal immunity toward intestinal microbiota. Interleukin-10-deficient (IL10(-/-)) mice develop Crohn's disease-like colitis unless raised germ-free conditions. Previous gas chromatography-mass spectrometry (GC-MS) metabolomic analysis revealed urinary metabolite differences between IL10(-/-) and wildtype C57BL/6 mice. To determine which these were specifically associated with inflammation arising...