Because ascorbate potentiates chemotaxis of normal leukocytes, we examined the effect on polymorphonuclear leukocytes from a patient with Chediak-Higashi syndrome. Chemotactic migration was 104+/-16 per 10 fields (mean+/-S.D.) initially and 258+/-44 (P less than 0.001) after ascorbate, as compared to 182+/-10 in controls. There no bactericidal activity by 40 minutes patient's untreated leukocytes. After control cells same. The addition reduced cAMP levels mean 34.5 pmoles 10(7) 5.9, value...

There is increasing evidence that many enzymes exist in tissues and cells multiple molecular forms (Markert Moller, 1959).Of less certainty, however, the question of differences between these terms their catalytic physiological activities.Indeed Markert Moller felt forms, termed isozymes, possessed identical substrate specificities although pattern isozymes by a tissue changed radically during development.The degree heterogeneity existing an enzyme one considerable importance.Such may be...

The administration of vitamin E (alpha-tocopherol) was found to improve polymorphonuclear leukocyte function in an infant with congenital deficiency glutathione synthetase activity. Before therapy the abnormal leukocytes exposed phagocytic challenge showed oxidant damage. They released 60 per cent more hydrogen peroxide than did normal leukocytes, iodinated only 20 25 number particles, and were unable kill bacteria as effectively although rates phagocytosis normal. These functional...

Biosynthesis of straight-chain ester volatiles by Granny Smith and Red Delicious apples was investigated using deuterium-labeled fatty acids, C-6 aldehydes, alcohols. Perdeuterated saturated monounsaturated acids were metabolized to hexyl-d11, hexanoate-d11, heptanoate-d13, octanoate-d15 esters, whereas perdeuterated linoleic acid produced only hexyl-d11 hexanoate-d11 esters. Exposure fruit vapors deuterated 3Z-hexenal, 2E-hexenal, hexanal identified the following biosynthetic processes: (1)...

To investigate the biochemical and cellular basis for rise in polymorphonuclear leukocyte (PMN) count during epinephrine administration, PMN from subjects receiving were studied their capacity to adhere nylon wool fibers endothelial cell monolayers. After administration of epinephrine, increased by 80% at 5 min, isolated adherence fell a base line 44±2-18±3%. In contrast, when infused with β-antagonist propanolol before failed was normal. Exposure monolayers 0.1 μM led diminished that could...

Summary The acid phosphatases of rat liver mitochondrial‐lysosomal fractions have been examined by quantitative and electrophoretic means. Disruption material freeze‐thawing, sonication, or blendor treatment released approximately 55.0 to 65.0 per cent the total phosphatase activity fraction into unsedimentable phase. Electrophoretic preparations this showed a single phosphatase‐active site. Treatment with 5.0 Triton X‐100 98.0 two major sites activity. One these was identical that resolved...

S ummary . Several episodes of neutropenia were observed in a child with glutathione synthetase deficiency (5‐oxoprolinuria). Studies the patient's glutathione‐deficient neutrophils undertaken to examine responses cells oxidative stress associated phagocytosis. The contained 10–20% normal content. Circulating infection‐free periods appeared less mature than by morphologic criteria, suggesting increased cell turnover. ingested particles, responded chemotactic stimuli, and oxidized 1‐ 14 C...

The properties and distribution of an enzyme in the epididymis mouse hydrolyzing thiamine pyrophosphate were investigated using cytochemical quantitative methods. Cytochemical study indicated pyrophosphatase to be restricted Golgi regions epithelial cells lining epididymal canal. Activity due this was easily separated from that alkaline phosphatase (which also hydrolyzed pyrophosphate) on basis differences localization greater sensitivity latter cysteine. present had optimal activity region...

Screening reduces colorectal cancer mortality, but many persons remain unscreened. with a blood test could improve screening rates. We estimated the comparative effectiveness and cost-effectiveness of emerging biomarkers, illustrated by methylated Septin 9 DNA plasma assay ((m)SEPT9), versus established strategies.We conducted cost-utility analysis using validated decision analytic model comparing (m)SEPT9, fecal occult testing (FOBT), immunochemical (FIT), sigmoidoscopy, colonoscopy,...

The ability of aliesterase, alkaline phosphatase, and acid phosphatase mouse epididymis separated in a starch matrix by electrophoresis to attack cytochemical substrates has been investigated tissues from normal, vasectomized, castrated, testosterone propionate treated animals. Electrophoresis at pH 8.5 resolved 7 enzymes active against α-naphthyl (designated A through G). Castration depressed the activity A, B, C; abolished D, E, G; increased F. Androgen administration restored all except...

Prior studies have shown disparities in the uptake of cardioprotective newer glucose-lowering drugs (GLDs), including sodium-glucose cotranwsporter-2 inhibitors (SGLT2i) and glucagon-like peptide-1 receptor agonists (GLP1a). This study aimed to characterize geographic variation initiation GLDs initiating these medications.

Achnatherum inebrians (drunken horse grass) causes symptoms in sheep and horses reminiscent of ergot alkaloid intoxication. Microscopical examination seed leaf tissues revealed the presence an endophytic fungus that did not produce spores when grown culture which was serologically related to Acremonium species Albo-lanosa section Morgan-Jones & Gams. ELISA indicated high concentrations alkaloids. Ergonovine lysergic acid amide were identified by HPLCat levels up 2500 400 mg kg-1,...

Differential and density equilibrium centrifugation have established the presence of alpha-hydroxy acid oxidase in microbodies kidney rat. The enzyme has been demonstrated cells distal convoluted tubule by a microscopic cytochemical method. This enzyme, like certain others microbodies, produces hydrogen peroxide.

Spermatozoa occupy several days in their passage through the cpididymal canal (Mason and Shaver, '52).During this time spermatozoa acquire increased fertilizing power (Young, '31).These facts suggest that epididymal cells secretory products may play a dual role.First, they actively participate differentiation of sperm ; secondly, must contribute to well-being by making available nutrients or otherwise maintaining compatible environment.Few attempts have been made study enzymatic...

Abstract Aims To characterize trends in the initiation of newer anti-obesity medications (AOMs) and determine factors associated with their use among obese/overweight populations. Materials methods This retrospective study utilized electronic health record data from OneFlorida+ (2015–2024). Adults eligible for AOMs were included, defined as having a BMI ≥30 kg/m² or 27–29.9 at least one obesity-related comorbidity. The primary outcome was AOMs, specifically glucagon-like peptide-1 receptor...

Abstract Aims To characterize trends in the initiation of newer anti‐obesity medications (AOMs) and determine factors associated with their use among obese/overweight populations. Materials Methods This study utilized electronic health record data from OneFlorida+ (2015–2024). Adults eligible for AOMs were included, defined as having a body mass index (BMI) ≥30 kg/m 2 or BMI 27–29.9 at least one obesity‐related comorbidity. The primary outcome was AOMs, specifically glucagon‐like peptide‐1...

The regulation of chromatin accessibility is essential in eukaryotic cells as one several mechanisms that ensure gene activation occurs at appropriate times and cell types. Accordingly, mutations remodeling proteins are linked to many different developmental disorders cancers. One example a protein has been both abnormalities cancer BPTF/NURF301, the largest subunit Nucleosome Remodeling Factor (NuRF) complex. BPTF not only important for formation NuRF but also helps direct its activity...