A5028266633- Plant and fungal interactions
- Botanical Research and Chemistry
- Metabolomics and Mass Spectrometry Studies
- Wheat and Barley Genetics and Pathology
- Plant Toxicity and Pharmacological Properties
- Turfgrass Adaptation and Management
- Genetic Mapping and Diversity in Plants and Animals
- Genetic and phenotypic traits in livestock
- Plant Molecular Biology Research
- Plant Gene Expression Analysis
- Plant Disease Resistance and Genetics
- Ruminant Nutrition and Digestive Physiology
- Plant nutrient uptake and metabolism
- Plant Pathogens and Resistance
- Plant Stress Responses and Tolerance
- Plant Taxonomy and Phylogenetics
- Gut microbiota and health
- Ginseng Biological Effects and Applications
- Plant pathogens and resistance mechanisms
- Plant biochemistry and biosynthesis
- Genomics and Phylogenetic Studies
- Advanced Chemical Sensor Technologies
- Analytical Chemistry and Chromatography
- Fermentation and Sensory Analysis
- Polysaccharides Composition and Applications
Kunming University
2025
AgResearch
2014-2024
Shanxi Agricultural University
2024
Xuzhou Central Hospital
2018
University of Missouri
2001-2003
Kyoto Prefectural University of Medicine
1991
DOI: 10.1111/j.1469-8137.2009.02771.x Commentary p 1
Liquid chromatography coupled to mass spectrometry (LCMS) is widely used in metabolomics due its sensitivity, reproducibility, speed and versatility. Metabolites are detected as peaks which characterised by mass-over-charge ratio (
ABSTRACT Drought is a major threat to alfalfa ( Medicago sativa L.) production. The discovery of important genes regulating drought response will facilitate breeding for drought‐resistant cultivars. Here, we report genome‐wide association study resistance in alfalfa. We identified and functionally characterized an MYB‐like transcription factor gene MsMYBH ), which increases the Compared with wild‐types, biomass forage quality were enhanced overexpressed plants. Combined RNA‐seq, proteomics...
Genomic prediction models for multi-year dry matter yield, via genotyping-by-sequencing in a composite training set, demonstrate potential genetic gain improvement through within-half sibling family selection. Perennial ryegrass (Lolium perenne L.) is key source of nutrition ruminant livestock temperate environments worldwide. Higher seasonal and annual yield herbage (DMY) principal breeding objective but the historical realised rate DMY modest. selection was investigated as tool to enhance...
A new powdery mildew resistance gene designated Pm21, from Haynaldia villosa, a relative of wheat, has been identified and incorporated into wheat through an alien translocation line. Cytogenetic biochemical analyses showed that chromosome arms 6VS 6AL were involved in this translocation. Random amplified polymorphic DNA (RAPD) analysis was performed on recipient cultivar Yangmai 5, the line, H. villosa with 180 random primers. Eight primers same results obtained four replications....
A fast method was developed to directly infuse raw plant extracts into a linear ion trap mass spectrometer, using the isolate and fragment as many ions possible from extract. The full spectra can be analysed by multivariate statistics determine discriminating ions, fragmentation data allows rapid classification or identification of these ions. methodology used screen wide range strains endophytic fungi in perennial ryegrass seeds for differences metabolic profiles. results show that this...
In perennial ryegrass (Lolium perenne L), annual and seasonal dry matter yield (DMY) nutritive quality of herbage are high priority traits targeted for improvement through selective breeding. Genomic prediction (GP) has proven to be a valuable tool improving complex may further enhanced the use multi-trait (MT) models. this study, we evaluated relative performance MT models improve predictive ability DMY key traits, using two different training populations (TP1, n = 463 TP2, 517) phenotyped...
Melilotus species are used as green manure and rotation crops worldwide contain abundant pharmacologically active coumarins. However, there is a paucity of information on its genome coumarin production function. Here, we reported chromosome-scale assembly albus with 1.04 Gb in eight chromosomes, containing 71.42% repetitive elements. Long terminal repeat retrotransposon bursts coincided declining population sizes during the Quaternary glaciation. Resequencing 94 accessions enabled insights...
Forage nutritive value impacts animal nutrition, which underpins livestock productivity, reproduction and health. Genetic improvement for traits in perennial ryegrass has been limited, as they are typically expensive time-consuming to measure through conventional methods. Genomic selection is appropriate such complex traits, enabling cost-effective prediction of breeding values using genome-wide markers. The aims the present study were assess potential genomic a range multi-population...
Cleistogenes songorica (2n = 4x 40) is a desert grass with unique dimorphic flowering mechanism and an ability to survive extreme drought. Little known about the genetics underlying drought tolerance its reproductive adaptability. Here, we sequenced assembled high-quality chromosome-level C. genome (contig N50 21.28 Mb). Complete assemblies of all telomeres, ten chromosomes were derived. underwent recent tetraploidization (~19 million years ago) four major chromosomal rearrangements....
Direct-infusion mass spectrometry (MS) was applied to study the metabolic effects of symbiosis between endophytic fungus Neotyphodium lolii and its host perennial ryegrass (Lolium perenne) in three different tissues (immature leaf, blade, sheath). Unbiased direct-infusion MS using a linear ion trap spectrometer allowed be determined free any preconceptions high-throughput fashion. Not only full MS(1) spectra (range 150-1,000 mass-to-charge ratio) were obtained but also MS(2) MS(3) product...
Abstract The identification of quantitative trait loci (QTL) for plant metabolites requires the quantitation these across a large range progeny. We developed rapid metabolic profiling method using both untargeted and targeted direct infusion tandem mass spectrometry (DIMSMS) with linear ion trap spectrometer yielding sufficient precision accuracy quantification number in high‐throughput environment. DIMSMS uses top‐down data‐dependent fragmentation MS 2 3 spectra. have software tools to...
The association between perennial ryegrass (
Metabolomics provides a powerful platform to characterize plants at the biochemical level, allowing search for underlying genes and associations with higher level complex traits such as yield nutritional value. Efficient reliable methods metabolic variation in economically important species are considered of high value evaluation prioritization germplasm breeding lines. In this investigation, large-scale metabolomic survey was performed on collection diverse perennial ryegrass (Lolium...
The interleukin-10-deficient (IL10(-/-)) mouse develops colon inflammation in response to normal intestinal microflora and has been used as a model of Crohn's disease. Short-Column LCMS metabolite profiling urine from IL10(-/-) wild-type (WT) mice was used, two independent experiments, identify mass spectral ions differing intensity between these genotypes. Three differential metabolites were identified xanthurenic acid the glucuronides α-CEHC...
Abstract Perennial ryegrass ( Lolium perenne ) is integral to temperate pastoral agriculture, which contributes most of the milk and meat production worldwide. Chemical profiles diversity offer several opportunities harness specific traits elucidate underlying biological mechanisms for forage improvement. We conducted a large-scale metabolomics study perennial comprising 715 genotypes, representing 118 populations from 21 countries. Liquid/gas chromatography–mass spectrometry based targeted...
In nematodes that invade the gastro-intestinal tract of ruminant, process larval exsheathment marks transition from free-living to parasitic stages these parasites. To investigate secretome associated with exsheathment, a closed in vitro system effectively reproduces two basic components an anaerobic rumen environment (CO2 and 39 °C) was developed trigger one most pathogenic model gastrointestinal nematodes, Haemonchus contortus (barber's pole worm). This study reports use multimodal...
Mass spectrometry coupled with chromatography has become the major technical platform in metabolomics. Aided by peak detection algorithms, detected signals are characterized mass-over-charge ratio (m/z) and retention time. Chemical identities often remain elusive for majority of signals. Multi-stage mass based on electrospray ionization (ESI) allows collision-induced dissociation (CID) fragmentation selected precursor ions. These fragment ions can assist structural inference metabolites low...
The efficiency of inorganic nitrogen (N) assimilation is a critical component fertilizer use by plants and forage production in Lolium perenne, an important pasture species worldwide. We present spatiotemporal description nitrate terms metabolic responses carbohydrate remobilization, together with components cytokinin signal transduction following addition to N-impoverished plants. Perennial ryegrass (L. perenne cv. Grasslands Nui) were grown for 10 weeks unfertilized soil then treated (5...