A5101713939- Plant Molecular Biology Research
- Plant Gene Expression Analysis
- Algal biology and biofuel production
- Plant Stress Responses and Tolerance
- Chromosomal and Genetic Variations
- Genomics and Phylogenetic Studies
- Genetic and Environmental Crop Studies
- Photosynthetic Processes and Mechanisms
- Plant Genetic and Mutation Studies
- Plant Reproductive Biology
- Plant biochemistry and biosynthesis
- Plant pathogens and resistance mechanisms
- Plant nutrient uptake and metabolism
- Ruminant Nutrition and Digestive Physiology
- Plant tissue culture and regeneration
- Plant Parasitism and Resistance
- Genetic diversity and population structure
- Plant and Fungal Species Descriptions
- Cancer-related molecular mechanisms research
- Botanical Research and Chemistry
- Microbial Natural Products and Biosynthesis
- Agronomic Practices and Intercropping Systems
- Proteins in Food Systems
- Heat shock proteins research
- Plant Virus Research Studies
Shandong Academy of Social Sciences
2025
Lanzhou University
2015-2024
Ministry of Agriculture and Rural Affairs
2018-2024
Institute of Geographic Sciences and Natural Resources Research
2024
University of Chinese Academy of Sciences
2024
Hubei Academy of Agricultural Sciences
2023
State Key Laboratory of Herbage Improvement and Grassland Agro-ecosystems
2016-2022
Institute of Grassland Research
2019-2022
Dalian Polytechnic University
2018
Ministry of Agriculture Livestock and Fisheries
2018
Significance Although auxin oxidation has long been known to be the primary mechanism of catabolism and Arabidopsis seedlings have 10–100 more 2-oxindole 3-acetic acid compared with other catabolic products, enzymes that constitutively catalyze this process remained unknown. This work fills gap by identifying characterizing proteins DIOXYGENASE FOR AUXIN OXIDATION 1 (DAO1) DAO2, which under normal growth conditions shows activity a physiological function in planta. The protein localization...
Water stress seriously constrains plant growth and yield. Long non-coding RNAs (lncRNAs) serve as versatile regulators in various biological regulatory processes. To date, the systematic screening potential functions of lncRNA have not yet been characterized Cleistogenes songorica, especially under water conditions.In this study, we obtained root shoot transcriptomes young C. songorica plants subjected to different degrees recovery treatments by Illumina-based RNA-seq. A total 3397 lncRNAs...
Abstract Melilotus is one of the most important legume forages, but lack molecular markers has limited development and utilization germplasm resources. In present study, 151 M clean reads were generated from various genotypes albus using Illumina sequencing. A total 19,263 potential EST-SSRs identified 104,358 unigene sequences. Moreover, 18,182 primer pairs successfully designed, 550 selected criteria base repeat type, fragment length annealing temperature. addition, screened by PCR...
Abstract Elephant grass (2 n = 4 x 28; Cenchrus purpureus Schumach.), also known as Napier grass, is an important forage and potential energy crop in tropical subtropical regions of Asia, Africa America. However, no study has yet reported a genome assembly for elephant at the chromosome scale. Here, we report high‐quality chromosome‐scale with total size 1.97 Gb 1.5% heterozygosity rate, obtained using short‐read sequencing, single‐molecule long‐read sequencing Hi‐C conformation capture....
Melilotus species are used as green manure and rotation crops worldwide contain abundant pharmacologically active coumarins. However, there is a paucity of information on its genome coumarin production function. Here, we reported chromosome-scale assembly albus with 1.04 Gb in eight chromosomes, containing 71.42% repetitive elements. Long terminal repeat retrotransposon bursts coincided declining population sizes during the Quaternary glaciation. Resequencing 94 accessions enabled insights...
Drought stress is one of the major constraints that decreases global crop productivity. Alfalfa, planted mainly in arid and semi-arid areas, crucial importance sustaining agricultural system. The family 1 UDP-glycosyltransferases (UGT) indispensable because it takes part regulation plant growth resistance. However, a comprehensive insight into participation UGT adaptation alfalfa to drought environments lacking. In present study, genome-wide analysis profiling were carried out. A total 409...
Rural revitalization is a strategic initiative in China aimed at addressing disparities between urban and rural areas by enhancing agriculture, livelihoods, infrastructure. Technological innovation serves as cornerstone of this effort. This study investigates the threshold effects four key external factors—openness to outside world, per capita economic development, educational input, government investment—on impact technological driving across China. A panel regression model was applied...
Melilotus albus is an annual or biennial legume species that adapts to extreme environments via its high stress tolerance. NAC and MYB transcription factors (TFs) are involved in the regulation of lignin biosynthesis, which has not been studied M. albus. A total 101 MaNAC 299 MaMYB members were identified based on genome. Chromosome distribution synteny analysis indicated some genes underwent tandem duplication. Ka/Ks suggested MaNACs MaMYBs strong purifying selection. Stress-, hormone-...
Plant growth and development depends on its ability to maintain optimal cellular homeostasis during abiotic biotic stresses. Cleistogenes songorica, a xerophyte desert plant, is known have novel drought stress adaptation strategies contains rich pools of tolerance genes. Proteins encoded by Late Embryogenesis Abundant (LEA) family genes promote activities functioning as disordered molecules, or limiting collisions between enzymes To date, functions the LEA been heavily investigated in many...
Cleistogenes songorica (2n = 4x 40) is a desert grass with unique dimorphic flowering mechanism and an ability to survive extreme drought. Little known about the genetics underlying drought tolerance its reproductive adaptability. Here, we sequenced assembled high-quality chromosome-level C. genome (contig N50 21.28 Mb). Complete assemblies of all telomeres, ten chromosomes were derived. underwent recent tetraploidization (~19 million years ago) four major chromosomal rearrangements....
Coumarins, natural products abundant in Melilotus albus, confer features response to abiotic stresses, and are mainly present as glycoconjugates. UGTs (UDP-glycosyltransferases) responsible for glycosylation modification of coumarins. However, information regarding the relationship between coumarin biosynthesis stress-responsive remains limited. Here, a total 189 MaUGT genes were identified from M. albus genome, which distributed differentially among its eight chromosomes. According...
Yellow sweet clover is a widely spread legume species that has potential to be used as forage crop in Western China. However, limited information available on the genetic variation for herbage yield, key morphological traits, and coumarin content. In this study, 40 half sib (HS) families of M. officinalis were evaluated genotypic phenotypic correlation traits: LS (leaf stem ratio), SV (spring vigor), LA area), PH (plant height), DW (herbage dry weight), SD (stem diameter), SN number), Cou...
The GRAS gene family is a plant-specific of transcription factors, which play an important role in many metabolic pathways, such as plant growth and development stress response. However, there no report on the comprehensive study Melilotus albus. Here, we identified 55 MaGRAS genes, were classified into 8 subfamilies by phylogenetic analysis, unevenly distributed chromosomes. structural analysis indicated that 87% genes have intron, highly conservative different species. proteins same...
Melilotus comprises 19 species, while the phylogenetic relationships between species remain unclear. In present work, three chloroplast genes, rbcL, matK, trnL-F, and one nuclear region, ITS (internal transcribed spacer) belonging to 48 populations of 18 were sequenced trees constructed study their interspecific relationships. Based on tree generated in this using rbcL analysis, genus is clearly monophyletic legume family. Both Bayesian maximum-parsimony approaches used analyze data. The...
Drought and high salinity are two major abiotic factors that restrict the productivity of alfalfa. By application Agrobacterium-mediated transformation method, an oxidative responsive gene, CsALDH12A1, from desert grass Cleistogenes songorica together with bar gene associated herbicide resistance, were co-transformed into alfalfa (Medicago sativa L.). From all 90 transformants, 16 positive as screened by spraying 1 mL L-1 10% Basta solution molecularly diagnosis using PCR. Real-time PCR...
Melilotus albus and officinalis are widely used in forage production herbal medicine due to the biological activity of their coumarins, which have many pharmacological activities, including anti-HIV anti-tumor effects. To comprehensively evaluate M. coumarin content (Cou), morphological variation, molecular phylogeny, we examined Cou, five traits characterization based on trnL-F spacer internal transcribed (ITS) regions 93 accessions. Significant (p < 0.05) variation was observed Cou all...
Coumarin and its derivatives are widely used as fragrances in industrial products have medical value. The goal of the present study was to discover genes pathways related coumarin biosynthesis Melilotus albus using transcriptome analysis. five M. near-isogenic lines (NILs) that had different content β-glucosidase activity according investigation pedigree were quantified then analysed by RNA-Seq. Using analysis, differentially expressed (DEGs) identified two pairwise comparisons differed well...
Melilotus, an annual or biennial herb, belongs to the tribe Trifolieae (Leguminosae) and consists of 19 species. As important green manure crop, diverse Melilotus species have different values as feed medicine. To identify species, we examined efficiency five candidate regions barcodes, including internal transcribed spacer (ITS) two chloroplast loci, rbcL matK, non-coding trnH-psbA trnL-F. In total, 198 individuals from 98 accessions representing 18 were sequenced for these potential...
Rabies remains a major public health concern in many developing countries. The precise neuropathogenesis of rabies is unknown, though it hypothesized to be due neuronal death or dysfunction. Mice that received intranasal inoculation an attenuated virus (RABV) strain HEP-Flury exhibited subtle clinical signs, and eventually recovered, which different from the fatal encephalitis caused by virulent RABV CVS-11. To understand mechanisms viral clearance, we applied RNA sequencing (RNA-Seq)...
Abstract Oyster protein isolate (OPI) suspensions (6.19 % ± 0.82 %, w/v) were treated by high-pressure homogenization (HPH) at 0 (control), 20, 40, 60, 80 or 100 MPa for three cycles. Protein profiles, secondary structure, free sulfhydryl, surface hydrophobicity, particle size distribution, zeta-potential, solubility, water and oil holding capacity (OHC), emulsifying foaming properties of the obtained analyzed. The results showed that HPH treatment did not cause changes in profiles OPI, but...