A5003062954- Plant Reproductive Biology
- Plant Physiology and Cultivation Studies
- Plant and animal studies
- Plant Molecular Biology Research
- Horticultural and Viticultural Research
- Plant nutrient uptake and metabolism
- Plant Gene Expression Analysis
- Hydraulic and Pneumatic Systems
- Biochemical and Structural Characterization
- Plant biochemistry and biosynthesis
- Plant and Fungal Interactions Research
- Nuts composition and effects
- Polysaccharides and Plant Cell Walls
- Plant Micronutrient Interactions and Effects
- Postharvest Quality and Shelf Life Management
- Advanced Sensor and Control Systems
- Phytochemicals and Antioxidant Activities
- Plant Stress Responses and Tolerance
- Plant tissue culture and regeneration
- Fire Detection and Safety Systems
- Photosynthetic Processes and Mechanisms
- Banana Cultivation and Research
- Geomechanics and Mining Engineering
- Flow Measurement and Analysis
- Nuclear Engineering Thermal-Hydraulics
Nanjing Agricultural University
2014-2023
First Affiliated Hospital of Henan University of Science and Technology
2018-2021
Shihezi University
2008-2014
SMC Corporation (Japan)
1999-2008
Southwest University of Science and Technology
2007
Union Hospital
2006
Huazhong University of Science and Technology
2006
Sophia University
1993
The draft genome of the pear ( Pyrus bretschneideri ) using a combination BAC-by-BAC and next-generation sequencing is reported. A 512.0-Mb sequence corresponding to 97.1% estimated size this highly heterozygous species assembled with 194× coverage. High-density genetic maps comprising 2005 SNP markers anchored 75.5% all 17 chromosomes. encodes 42,812 protein-coding genes, these, ∼28.5% encode multiple isoforms. Repetitive sequences 271.9 Mb in length, accounting for 53.1% genome, are...
Pear (Pyrus) is a globally grown fruit, with thousands of cultivars in five domesticated species and dozens wild species. However, little known about the evolutionary history these pear what has contributed to distinct phenotypic traits between Asian pears European pears.
SWEET genes are a recently identified plant gene family that play an indispensable role in sugar efflux.However, no systematic study has been performed pear.In this research, 18 transporters pear, almost twice the number found woodland strawberry and Japanese apricot, were divided into four clades.Conserved motifs six exons of species.SWEET contained seven transmembrane segments (TMSs) evolved from internal duplication ancestral three-TMSs unit, connected by TMS4.This is first direct...
To examine the biochemical metabolism of aroma volatiles derived from fatty acids, pear fruits were incubated in vitro with metabolic precursors these compounds. Aroma volatiles, especially esters, significantly increased, both qualitatively and quantitatively, fed on acid precursors. Cultivars having different flavor characteristics had distinctly volatile metabolisms. More esters formed fruity-flavored “Nanguoli” than green-flavored “Dangshansuli” same quantities linoleic linolenic acid....
Polygalacturonase (PG), as an important hydrolase participating in the degradation of pectin, plays role softening process fruit. However, information on PG gene family pear genome and specific member involved fruit is still rudimentary.In this study, a total 61 genes, which could be divided into six subclasses, were identified from with diverse chromosome locations, structures, motifs cis-acting elements. Most PbrPGs derived WGD/segmental duplication blocks, purifying selection was main...
Tonoplast monosaccharide transporters (TMTs) play important roles in vacuolar sugar accumulation plants. In this study, six TMT genes (PbTMT1-6) were identified the Pyrus bretschneideri genome database, and their expression profiles correlated with soluble contents during pear (P. cv. Ya Li) fruit development process. Subsequently, PbTMT4 was as a strong contributor to fructose, glucose sucrose fructescence of pears. Heterologous PbTMT4, hexose transporter-deficient yeast strain EBY.VW4000,...
The K+ transporter/high-affinity K+/K+ uptake (KT/HAK/KUP) family, as one of the largest transporter families in higher plants, plays an essential role plant growth, mineral element absorption, salt stress tolerance, and other physiological processes. However, little is known about this family pear (Pyrus). Here, we identified 20 genes (P. bretschneideri) using genome-wide analysis. Their gene structure, chromosomal distribution, conserved motifs, phylogenetics, duplication events,...
Cysteine-rich peptides (CRPs) are gaining recognition as regulators of cell-cell communication in plants.We identified 9556 CRPs 12 plant species and analysed their evolutionary patterns. In most angiosperm plants, whole genome duplication segmental the major factors driving expansion CRP family member genes, especially signal peptides. About 30% genes were found clustered on chromosomes, except maize (Zea mays). Considerable collinearities between or within reveal several syntenic regions...
Abstract Sucrose accumulation is one of the important factors that determine fruit enlargement and quality. Evaluation sugar profile 105 pear cultivars revealed low-sucrose high-sucrose (HS) types fruits. To better understand molecular mechanisms governing sucrose content fruits, this study performed transcriptome analysis during development using ‘Korla’ fragrant HS ‘Hosui’ pear, a coexpression module uniquely associated with control was identified by weighted gene network analysis. These...
Sucrose is an important factor affecting sweetness and flavor in pear fruits, but the molecular mechanism of sucrose synthesis regulation relatively unknown. Here, we characterized a transcription gene from (Pyrus pyrifolia Nakai cv. "Hosui") PpybZIP43, found that transient overexpression PpybZIP43 fruits significantly increased content relative expression level phosphate synthase genes (PpySPS3 PpySPS8). Subcellular localization analysis tobacco leaves showed was localized nucleus. Yeast...
Ascorbic acid (AsA) is a major antioxidant and redox buffer in plants. Dehydroascorbate reductase (DHAR; EC 1.8.5.1) catalyzes the conversion of dehydroascorbate (DHA) to AsA crucial for regeneration. In this study, we developed transgenic tomato plants that overexpressed PbDHAR2 investigate whether could limit deleterious effects salt chilling stresses. These contained significantly higher levels than wild-type (WT) Overexpression increased expression AsA-glutathione (GSH) cycle genes lines...
Vacuolar processing enzymes (VPEs) are important cysteine proteases and function in the maturation of protein precursors, plant senescence immunity, programmed cell death, as well sugar accumulation. This paper investigated VPEs pear identified a key finding eight members VPE family (Pyrus) genome, their gene structure, conserved domains phylogenetic relationship were analysed by bioinformatics methods. Further findings that two these (PbrVPE2 PbrVPE5) represented vegetative others seed-coat...
UDP glycosyltransferases (UGTs) play an indispensable role in regulating signaling pathways and intracellular homeostasis plants by catalyzing the glycosylation of metabolites. To date, molecular characteristics potential biological functions UGT gene family pomegranate (Punica granatum L.) remain elusive. In this study, a total 120 PgUGT genes were identified genome. Phylogenetic analysis revealed that these PgUGTs clustered into 15 groups: 13 conserved groups (A–J L–N) two newly discovered...
ATP-binding cassette subfamily G (ABCG) proteins play important roles in plant growth and development by transporting metabolites across cell membranes. To date, the genetic characteristics potential functions of pomegranate ABCG (PgrABCGs) have remained largely unknown. In this study, we found that 47 PgrABCGs were divided into five groups according to a phylogenetic analysis; I, II, III, IV members are half-size proteins, group V full-size proteins. PgrABCG14, PgrABCG21, PgrABCG47 highly...