A5012544868- RNA and protein synthesis mechanisms
- Chronic Myeloid Leukemia Treatments
- Liver physiology and pathology
- Biochemical Analysis and Sensing Techniques
- Cancer Genomics and Diagnostics
- Chronic Lymphocytic Leukemia Research
- Mast cells and histamine
- Eosinophilic Disorders and Syndromes
- RNA Research and Splicing
- Immune Cell Function and Interaction
- Mycobacterium research and diagnosis
- RNA modifications and cancer
- Genetic factors in colorectal cancer
- RNA regulation and disease
- Sulfur Compounds in Biology
- T-cell and B-cell Immunology
- Acute Myeloid Leukemia Research
- Immune Response and Inflammation
- Lymphoma Diagnosis and Treatment
- Genetics and Neurodevelopmental Disorders
- Rice Cultivation and Yield Improvement
- Microbial infections and disease research
- Single-cell and spatial transcriptomics
- Multiple Myeloma Research and Treatments
- PI3K/AKT/mTOR signaling in cancer
Medical University of Vienna
2006-2025
Centre for Genomic Regulation
2015-2021
Centro Nacional de Análisis Genómico
2012-2021
University of Vienna
2001-2020
Universitat Pompeu Fabra
2015-2017
Barcelona Institute for Science and Technology
2017
Instituto de Biomedicina y Biotecnología de Cantabria
2015
Cukurova University
2013
Hospital Universitario Puerta de Hierro Majadahonda
2013
German Cancer Research Center
2008
Abstract As whole-genome sequencing for cancer genome analysis becomes a clinical tool, full understanding of the variables affecting output is required. Here using tumour-normal sample pairs from two different types cancer, chronic lymphocytic leukaemia and medulloblastoma, we conduct benchmarking exercise within context International Cancer Genome Consortium. We compare methods, pipelines validation methods. show that PCR-free methods increasing depth to ∼100 × shows benefits, as long...
High-density SNP arrays for genome-wide assessment of allelic variation have made high resolution genetic characterization crop germplasm feasible. A medium density array apple, the IRSC 8 K array, has been successfully developed and used screens bi-parental populations. However, number robust well-distributed markers contained on this was not sufficient to perform association analyses in wider sets, or Pedigree-Based Analysis at precision, because rapid decay linkage disequilibrium. We...
BackgroundThe Mediterranean olive tree (Olea europaea subsp. europaea) was one of the first trees to be domesticated and is currently major agricultural importance in region as source oil. The molecular bases underlying phenotypic differences among cultivars, or between their wild relatives, remain poorly understood. Both cultivated have 46 chromosomes (2n).
Genomic studies of endangered species provide insights into their evolution and demographic history, reveal patterns genomic erosion that might limit viability, offer tools for effective conservation. The Iberian lynx (Lynx pardinus) is the most felid a unique example on brink extinction.
Abstract In an effort to characterize molecules with immunoregulatory potential, we raised mAbs human dendritic cells. We selected Ab that recognizes a molecule is induced on monocytes differentiated in vitro toward Retroviral expression cloning identified this as B7-H3, member of the B7 family described recently. contrast earlier report, which B7-H3 was consisting two Ig-like domains, our cDNA encoded type I membrane protein four and by us therefore named 4Ig-B7-H3. mRNA analysis well...
After its domestication, rice cultivation expanded from tropical regions towards northern latitudes with temperate climate in a progressive process to overcome limiting photoperiod and temperature conditions. This has originated wide range of diversity that can be regarded as valuable resource for crop improvement. In general, current breeding programs have deal lack both germplasm accessions specifically adapted local agro-environmental conditions donors carrying desired agronomical traits....
Merkel cell carcinoma (MCC) is a highly malignant neuroendocrine tumor of the skin whose molecular pathogenesis not completely understood, despite role that polyomavirus can play in 55–90% cases. To study potential mechanisms driving this disease clinically characterized cases, we searched for somatic mutations using whole-exome sequencing, and extrapolated our findings to functional biomarkers reporting on activity mutated pathways. Confirming previous results, polyomavirus-negative tumors...
Adenosine deaminase acting on RNA ADAR1 promotes A-to-I conversion in double-stranded and structured RNAs. has two isoforms transcribed from different promoters: cytoplasmic ADAR1p150 is interferon-inducible while ADAR1p110 constitutively expressed primarily localized the nucleus. Mutations cause Aicardi - Goutières syndrome (AGS), a severe autoinflammatory disease associated with aberrant IFN production. In mice, deletion of or p150 isoform leads to embryonic lethality driven by...
The NK gene complex is a region on human chromosome 12 containing several families of lectin-like genes including the CD94 and NKG2 receptor genes. We report here that telomeric contains in addition to LOX-1 novel DECTIN-1 CLEC-1 CLEC-2 within about 100 kb. Sequence similarities chromosomal arrangement suggest these form separate subfamily complex. selectively expressed dendritic cells lower extent monocytes macrophages. mRNA forms with without stalk exon are observed. During functional...
Abstract Chronic myeloid leukemia (CML) is a myeloproliferative disease in which BCR/ABL enhances survival of leukemic cells through modulation proapoptotic and antiapoptotic molecules. Recent data suggest that Bcl-2–interacting mediator (Bim) plays role as tumor suppressor cells, express only low amounts this cell death activator. We here show primary CML significantly lower bim mRNA Bim protein compared with normal cells. The inhibitors imatinib AMN107 were found to promote expression To...
The ectonucleotidase CD39 on human regulatory T-cells (Treg) is an important immune regulator which dysregulated in autoimmune diseases and cancer immunosuppression. We here define that expression Treg independent of the Treg-specific transcription factors FOXP3 HELIOS promoted by canonical TGF-b- mTOR-signaling. Furthermore, TGF-b mediated upregulation counteracted reactive oxygen species (ROS)-driven autophagy. In line, CD39+ peripheral blood constitute a distinct lineage with low...
Dendritic cell (DC) development is orchestrated by lineage-determining transcription factors (TFs). Although, members of the activator-protein-1 (AP-1) family, including Batf3, have been implicated in conventional (c)DC specification, role Jun proteins poorly understood. Here, we identified c-Jun and JunB as essential for cDC1 fate specification function. In mice, regulate extrinsic intrinsic pathways, which control CD8α diversification, whereas CD103 unaffected. The loss DC progenitors...
Alcohol-related liver disease (ALD) remains a major global health burden with limited therapeutic options due to an incomplete understanding of its underlying molecular mechanisms and cellular crosstalk. Here, we applied ultra-high resolution (on 2μm spots) spatial transcriptomics cirrhotic tissue obtained from end-stage ALD patient, analyzing >265,000 spatially resolved cells further validation on single-cell single-nuclei datasets patients cirrhosis. Our analysis delineated distinct...
The mammalian target of rapamycin (mTOR) has recently been described to be constitutively activated in Bcr-Abl-transformed cells and mediate rapamycin-induced inhibition growth respective cell lines. We have shown that down-regulates expression vascular endothelial factor (VEGF), a mediator leukemia-associated angiogenesis, primary CML cells. In the present study, we analyzed growth-inhibitory vitro vivo effects on asked whether suppression VEGF leukemic is related inhibition. Rapamycin dose...
Many infectious viruses coevolved with the vertebrate immune system. During assembly of enveloped viruses, lipid ordered domains host cell plasma membrane, called rafts, frequently function as a natural meeting point for viral proteins. The role rafts in organization complex combinations receptors during antigen presentation and T signaling is widely recognized. In our studies, we determined whether virus budding, molecular interactions activation could be brought into novel context to...
Training of the innate immune system with orally ingested bacterial extracts was demonstrated to have beneficial effects on infection clearance and disease outcome. The aim our study identify cellular molecular processes responsible for these immunological benefits. We used a murine coronavirus (MCoV) A59 mouse model treated activating extract Broncho-Vaxom (BV) OM-85. Tissue samples were analysed qPCR, RNA sequencing, histology, flow cytometry. After BV OM-85 treatment, interstitial...
Mesenchymal stromal cells (MSCs) are multipotent, progenitor that reside in tissues across the human body, including periodontal ligament (PDL) and gingiva. They a promising therapeutic tool for various degenerative inflammatory diseases. However, different heterogeneity levels caused by tissue-to-tissue donor-to-donor variability, even intercellular differences within given MSCs population, restrict their potential. There considerable efforts to decipher these using “omics” approaches,...
ABSTRACT Viral particles preferentially incorporate extra- and intracellular constituents of host cell lipid rafts, a phenomenon central to pseudotyping. Based on this mechanism, we have developed system for the predictable decoration enveloped viruses with functionally active cytokines that circumvents need modify viral proteins themselves. Human interleukin-2 (hIL-2), hIL-4, human granulocyte-macrophage colony-stimulating factor (hGM-CSF), murine IL-2 (mIL-2) were used as model fused at...