A5042634147- Viral Infections and Vectors
- Vector-borne infectious diseases
- Vector-Borne Animal Diseases
- Anesthesia and Neurotoxicity Research
- Nanoparticles: synthesis and applications
- DNA Repair Mechanisms
- Insect and Pesticide Research
- Toxoplasma gondii Research Studies
- Aquaculture Nutrition and Growth
- RNA modifications and cancer
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Environmental Toxicology and Ecotoxicology
- Fish Biology and Ecology Studies
- Muscle metabolism and nutrition
- Neonatal and fetal brain pathology
- Medicinal Plants and Neuroprotection
- MicroRNA in disease regulation
- Adipose Tissue and Metabolism
- Chromosomal and Genetic Variations
- RNA Research and Splicing
- Nanoparticle-Based Drug Delivery
- Bird parasitology and diseases
- Neuroscience and Neuropharmacology Research
- Biochemical effects in animals
- Reproductive Biology and Fertility
Bahauddin Zakariya University
2016-2025
Indiana University
2025
University School
2025
Indiana University School of Medicine
2025
Islamia University of Bahawalpur
2024
Abdul Wali Khan University Mardan
2023-2024
Chia-Yi Christian Hospital
2023
Chia Nan University of Pharmacy and Science
2023
National Chung Hsing University
2023
National Cheng Kung University
2023
Abstract Endometriosis, a pathological condition in which the endometrium grows outside uterus, is one of most common causes female infertility; it diagnosed 25–40% infertile women. The mechanism by endometriosis affects fertility females remains largely unknown. We examined ultrastructure oocytes from patients with minimal or mild and control undergoing vitro fertilization (IVF) treatment transmission electron microscopy (TEM) to investigate physiological significance oocyte quality for...
Fanconi anemia (FA) genes play important roles in spermatogenesis. In mice, disruption of Fancm impairs male fertility and testicular integrity, but whether FANCM pathogenic variants (PV) similarly affect men is unknown. Here we characterize a Pakistani family having three infertile brothers, two manifesting oligoasthenospermia one exhibiting azoospermia, born to first-cousin parents. A homozygous PV (c.1946_1958del, p.P648Lfs*16) was found cosegregating with infertility. Our objective...
Anaplasmosis, caused by intracellular gram-negative bacteria Anaplasma marginale is one of the most frequently reported tick-borne disease (TBDs) in tropical and sub-tropical countries, including Pakistan. In present study, a total 428 cattle blood samples were collected to examine prevalence phylogenetic origin A. two important livestock regions Punjab Province Pakistan, i.e. Lodhran Dera Ghazi Khan Districts. addition, association between occurrence selected epidemiological factors has...
Multiple morphological abnormalities of the sperm flagella (MMAF) are most severe form asthenozoospermia due to impaired axoneme structure in flagella. Dynein arms necessary components flagellar axoneme. In this study, we recruited 3 unrelated consanguineous Pakistani families with multiple MMAF-affected individuals, who had no overt ciliary symptoms. Whole-exome sequencing and Sanger identified 2 cilia associated protein 57 (CFAP57) loss-of-function mutations (c.2872C>T, p. R958*;...
Gastric cancer is the fourth most commonly diagnosed with second highest mortality rate worldwide. Surgery, chemotherapy and radiation therapy are generally used for treatment of stomach but only limited clinical response shown by these therapies still no effectual advanced gastric adenocarcinoma patients available. Therefore, there a need to identify other therapeutic agents against this life-threatening disease. Plants considered as one important sources development anticancer drugs....
This study investigates the molecular prevalence and phylogenetic characteristics of two prominent blood-borne pathogens, Toxoplasma gondii (T. gondii) Plasmodium spp., in common quails (Coturnix coturnix) sampled from both wild (N = 236) farmed 197) populations across four districts (Layyah, Dera Ghazi Khan, Lahore, Multan) Punjab, Pakistan, during hunting seasons 2021 to 2023. Additionally, impact these pathogens on complete blood count (CBC) hosts is examined. Out 433 tested, 25 (5.8%)...
To determine the presence of Babesia bovis (B. bovis) in large ruminants southern Punjab and its effect on hematological serum biochemical profile host animals.Blood samples were collected from 144 ruminants, including 105 cattle 39 buffaloes, six districts Multan, Layyah, Muzaffar Garh, Bhakar, Bahawalnagar Vehari. Data characteristics animals herds through questionnaires. Different blood (hemoglobin, glucose) (ALT, AST, LDH, cholesterol) parameters calves measured compared between parasite...
: Next-Generation Sequencing (NGS) technology has revealed that microRNAs (miRNAs) are capable of exhibiting frequent differences from their corresponding mature reference sequences, generating multiple variants: the isoforms miRNAs (isomiRs). These isomiRs mainly originate via imprecise and alternative cleavage during pre-miRNA processing post-transcriptional modifications influence miRNA stability, sub-cellular localization target selection. Although several tools for identification isomiR...
CDH2 (cadherin 2, Neural-cadherin, or N-cadherin) is the predominant protein of testicular basal ectoplasmic specializations (basal ES; a testis-specific type adhesion junction), one major cell junctions composing blood-testis barrier (BTB). The BTB found between adjacent Sertoli cells in seminiferous tubules, which divides tubules into and adluminal compartments prevents deleterious exchange macromolecules blood tubules. However, exact roles ES function spermatogenesis still unknown. We...
Titanium, Aluminum and Vanadium (Ti 6Al 4V) alloy are frequently used as surgical implant but regarding their compatibility in living systems is limited. Ti 4V was prepared from high purity constituents powder (25 mg/ml solvent/Kg body weight) gavaged to albino mice for 8 days. A saline treated control group maintained parallel. series of behavioral (rota rod, light dark box, open field novel object) test performance, complete blood count, selected serum (HDL cholesterol, LDL creatinine,...
Majority of Pakistani population lives in rural areas and raising animals, especially the small ruminants, is their primary source income. Anaplasma ovis known to infect ruminants globally causing significant economic losses livestock owners, however prevalence has been least investigated from Pakistan despite having a huge sheep population.The present study was conducted June 2021 till December report PCR based blood samples (n = 239) that were collected District Dera Ghazi Khan...
To report and compare the hematological serum biochemical profile of goat sheep from Rahim Yar Khan district in Southern Punjab.One hundred twenty blood samples (98 22 sheep) were collected their glucose, hemoglobin parameters, cholesterol, aspartate transaminase (AST), alanine aminotransferase (ALT) lactate dehydrogenase (LDH) determined by using spectrophotometer. Parameters compared between sheep.It was observed that glucose (P=0.001), LDH (P=0.001) ALT concentrations differed...
Small RNA (sRNA) Sequencing technology has revealed that microRNAs (miRNAs) are capable of exhibiting frequent variations from their canonical sequences, generating multiple variants: the isoforms miRNAs (isomiRs). However, integrated tool to precisely detect and systematically annotate isomiRs sRNA sequencing data is still in great demand. Here, we present an online tool, DeAnnIso (Detection Annotation IsomiRs data). can all uploaded sample, extract differentially expressing paired or...
Anaplasma marginale ( A . ), ovis ) and Theileria T are among the most commonly reported intracellular tick borne pathogens that infect ruminants across globe causing huge economic losses. This study aims to report prevalence phylogenetic evaluation of these three infecting sheep goats (n = 333) were enrolled from Fort Munro region in Pakistan by using msp1b , msp4 18S rRNA genes for respectively. Results revealed almost similar infection rates with an overall 11% 28% 3% Concurrent was also...
The Common House Crow (Corvus splendens) exhibits remarkable ecological adaptability, enabling its rapid expansion across continents. However, despite wide distribution, there is a need for genetic studies to clarify evolutionary history and population structure. This research employs DNA barcoding, focusing on the mitochondrial gene cytochrome oxidase subunit I (Cox1), which effective species identification phylogenetic analysis. Blood samples were collected from 70 C. splendens specimens...
Despite of having rich reptilian fauna, the wild lizards from Pakistan remained unexplored for presence blood borne parasites. The present study was designed to report molecular epidemiology and phylogenetic evaluation various pathogens (Hepatozoon sp., Schellackia spp., Toxoplasma gondii, Plasmodium Haemoproteus spp. Leucocytozoon spp.) in samples (N = 84), trapped during March 2022 till June 2023 district Karak Dir Khyber Pakhtunkhwa (Pakistan). Seven lizard species were identified study....