A5103000214- Alzheimer's disease research and treatments
- Neuroscience and Neuropharmacology Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Bioinformatics and Genomic Networks
- Neurological Disorders and Treatments
- Neurological Disease Mechanisms and Treatments
- Phytochemistry and Biological Activities
- Ginkgo biloba and Cashew Applications
- Plant Reproductive Biology
- Functional Brain Connectivity Studies
- Parkinson's Disease Mechanisms and Treatments
- Plant pathogens and resistance mechanisms
- Optical Imaging and Spectroscopy Techniques
- Molecular Biology Techniques and Applications
- Ferroptosis and cancer prognosis
- Protease and Inhibitor Mechanisms
- Chemical Synthesis and Analysis
- GDF15 and Related Biomarkers
- Traditional Chinese Medicine Analysis
- MicroRNA in disease regulation
- Environmental DNA in Biodiversity Studies
- Biochemical effects in animals
- Computational Drug Discovery Methods
- Medicinal Plants and Bioactive Compounds
- Free Radicals and Antioxidants
Nanjing Agricultural University
2021-2025
Shanghai University of Traditional Chinese Medicine
2015-2024
Longhua Hospital Shanghai University of Traditional Chinese Medicine
2015-2024
Shanxi Datong University
2024
Xi'an University of Science and Technology
2024
Creative Commons
2021
Weatherford College
2021
Mongolian National University of Medical Sciences
2020
Second Military Medical University
2015
Changhai Hospital
2015
Abstract The S-RNase-based self-incompatibility locus ( S -locus) in Petunia species contains 16–20 F-box genes, which collaboratively function the recognition and subsequent degradation of non-self S-RNases, while distinguishing them from self S-RNase. However, number -locus F - box genes SFBB s) physically interacted with S-RNases remains uncertain Pyrus species. Utilizing Pacbio long-read sequencing, we successfully assembled genome pear cultivar ‘Yali’ bretschneideri ), identified 19 s...
Background: The development of Alzheimer’s disease (AD) can be divided into subjective cognitive decline (SCD), mild impairment (MCI), and dementia. Early recognition pre-AD stages may slow the progression Objective: This study aimed to explore functional connectivity (FC) changes brain prefrontal cortex (PFC) in AD continuum using near-infrared spectroscopy (fNIRS), analyze its correlation with function. Methods: All participants underwent 48-channel fNIRS at resting-state. Based on...
Context Ursolic acid (UA) and acteoside (ATS) are important active components that have been used to treat Alzheimer's disease (AD) because of their neuroprotective effects, but the exact mechanism is still unclear.Objective Network pharmacology was explore UA + ATS in treating AD, cell experiments were verify mechanism.Materials methods targets AD-related genes retrieved from TCMSP, STITCH, SwissTargetPrediction, GeneCards, DisGeNET GEO. Key obtained by constructing protein interaction...
Abstract Background Shanzhuyu (the dried mature sarcocarp of Cornus officinalis Sieb. et Zucc., DMSCO) is a Chinese herb that can be used for the treatment Alzheimer’s disease (AD), but its mechanism remains unknown. The present study aimed to investigate active ingredients and effective mechanisms DMSCO AD based on network pharmacology approach. Methods components were collected from TCMSP ETCM databases target proteins these compounds predicted using TCMSP, SwissTargetPrediction STITCH...
To study the effects of inhibited Annexin A2 (ANXA2) on human umbilical vein endothelial cells (HUVECs) in vitro.Short hairpin RNA (shRNA) targeting ANXA2 was designed and cloned into double marked lentivirial vector GV248 for RNAi to generate recombinant expression plasmids, which were stably transfected HUVECs. The protein mRNA levels analyzed by western blotting real-time polymerase chain reaction, respectively. Cell proliferation (cell counting kit-8 assay), apoptosis (flow cytometry...
Multiple sclerosis (MS) is a chronic inflammatory disease of central nervous system leading to demyelination followed by neurological symptoms. Ferroptosis newly discovered pathogenic hallmark important for the progression MS. However, gene markers ferroptosis in MS are still uncertain. In this study, mRNA expression profiles and clinical data samples were retrieved from Gene Expression Omnibus database. Weighted co-expression network analysis receiver operating characteristic curve utilized...
In this study, gene expression profiles in peripheral blood monocytes from patients with Alzheimer's disease (AD) or mild cognitive impairment (MCI) were compared those of healthy individuals to identify key differentially expressed genes (DEGs), an effort broaden our understanding the pathogenesis these diseases and potential therapeutic targets. The microarray data set GSE18309 was downloaded Gene Expression Omnibus, including 3 AD, MCI normal control (NC) samples. Raw processed...
Traditional Chinese medicine can be used for Alzheimer's disease management, such as the modern herbal formula Di-Huang-Yi-Zhi (DHYZ). In present study, neuronal differentiated PC12 cells were a model to evaluate effects of DHYZ against amyloid-β peptide 25-35 (Aβ25-35) induced neurotoxicity, particularly regarding cell proliferation, apoptosis and related events. Following treatment with DHYZ, viability, membrane damage, apoptosis, mitochondrial potential, cytochrome c release, caspase-3...
Abstract Background Oxidative stress mediates the nerve injury during pathogenesis of Alzheimer’s disease (AD). Protecting against oxidative damage is an important strategy to prevent and treat AD. Di-Huang-Yi-Zhi (DHYZ) a Chinese medicine used for treatment AD, but its mechanism remains unknown. This study aimed investigate effect DHYZ on H 2 O induced in PC12 cells. Methods cells were treated with DHYZ. Cell proliferation was detected by counting kit-8 (CCK-8) assay. Cytotoxicity measured...
Abstract Background: Mild cognitive impairment (MCI), as a common neurodegenerative aging disease representing an intermediate stage between normal functioning and dementia, poses excessive burden on health care. The clinical benefit of Chinese herbal medicines (CHMs) for MCI remains inconclusive. This study is aimed at evaluating the efficacy acceptability CHMs through meta-analysis trial sequential analysis (TSA). Methods: We applied extensive strategies preliminary literature screening to...
Abstract Background— The active monomer Verbascum glycosides in Cistanche tubulosa has good development prospects terms of neuroprotection and delaying neurodegenerative diseases, it become one the research hot spots. To investigate effect verbascoside (OC1) on expression phosphorylated protein protective AD cell model by TMT labeling phosphorylation enrichment technique high-resolution liquid chromatography-mass spectrometry quantitative proteomics strategy. Methods— normal control group,...
Abstract Background : Quantitative real-time PCR (qRT-PCR) is currently one of the most reliable and improved tools for analyzing gene expression. Various studies have shown that housekeeping genes was varied with cultivars, tissues treatment. The stable reference were necessarily identified evaluated according to different experimental requirements. Result In this study, 10 candidate initially screened based on transcriptome sequencing data four pear fruit development stages three including...