A5081650906- Microtubule and mitosis dynamics
- Fungal and yeast genetics research
- DNA Repair Mechanisms
- Plant nutrient uptake and metabolism
- Biofuel production and bioconversion
- Metabolism, Diabetes, and Cancer
- Ubiquitin and proteasome pathways
- Cellular Mechanics and Interactions
- Cellular transport and secretion
- Mitochondrial Function and Pathology
- Genomics and Chromatin Dynamics
- Mass Spectrometry Techniques and Applications
- Polysaccharides and Plant Cell Walls
- Advanced Proteomics Techniques and Applications
- Genetic Neurodegenerative Diseases
- Metabolomics and Mass Spectrometry Studies
- Hippo pathway signaling and YAP/TAZ
University of Vermont
2021-2024
Dartmouth College
2017-2020
Duke University
2017
Saint Michael's College
2012
Background: The mitotic kinesin, KIF18A, is required for proliferation of cancer cells that exhibit chromosome instability (CIN), implicating it as a promising target treatment subset aggressive tumor types. Determining regions the KIF18A protein to inhibition will be important design and optimization effective small molecule inhibitors. Methods: In this study, we used cultured cell models investigate effects mutating S284 within alpha-4 helix which was previously identified phosphorylated...
Kinesin-binding protein inhibits kinesin activity through structural alterations of the motor domain.
The heterotrimeric kinase AMPK acts as an energy sensor to coordinate cell metabolism with environmental status in species from yeast through humans. Low intracellular ATP leads activation phosphorylation of the loop within catalytic subunit. Other stresses also activate AMPK, but it is unclear whether cellular affects under these conditions. Fission subunit Ssp2 phosphorylated at Thr-189 by upstream Ssp1 low-glucose conditions, similar other systems. Here we find that hyperosmotic stress...
AMPK-related protein kinases (ARKs) coordinate cell growth, proliferation, and migration with environmental status. It is unclear how specific ARKs are activated at times. In the fission yeast
Abstract A hallmark of cell division in eukaryotic cells is the formation and elongation a microtubule (MT)‐based mitotic spindle. Proper positioning spindle critical to ensure equal segregation genetic material resulting daughter cells. Both timing constriction actomyosin contractile ring must be precisely coordinated prevent missegregation or damage during cellular division. Here, we show that Sds23, an inhibitor protein phosphatases, contributes proper elongating spindles fission yeast We...
Animal and fungal cells divide through the assembly, anchoring, constriction of a contractile actomyosin ring (CAR) during cytokinesis. The timing position CAR must be tightly controlled to prevent defects in cell division, but many underlying signaling events remain unknown. conserved heterotrimeric protein phosphatase PP2A controls mitosis, upstream pathways including Greatwall–Ensa regulate activity. A role for regulation has been less clear, although loss yeast causes Here, we report...
Abstract Background: Chromosome instability (CIN), characterized by frequent and ongoing loss or gain of chromosome number, is commonly observed in tumor cells. Although long recognized as a vulnerability cancer cells, potential CIN-selective therapeutic targets have only recently been discovered. Genetic studies from multiple groups identified the mitotic kinesin, KIF18A, selectively essential for proliferation CIN aneuploid By targeting KIF18A genetically with novel small molecule...
ABSTRACT The mitotic kinesin, KIF18A, is required for proliferation of cancer cells that exhibit chromosome instability (CIN), implicating it as a promising target treatment subset aggressive tumor types. Determining regions the KIF18A protein to inhibition will be important design and optimization effective small molecule inhibitors. In this study, we investigated effects mutating S284 within alpha-4 helix which was previously identified phosphorylated residue. Mutations in cause...
Abstract Animal and fungal cells divide through the assembly, anchoring, constriction of a contractile actomyosin ring (CAR) during cytokinesis. The timing position CAR must be tightly controlled to prevent defects in cell division, but many underlying signaling events remain unknown. conserved heterotrimeric protein phosphatase PP2A controls mitosis, upstream pathways including Greatwall-Ensa regulate activity. A role for regulation has been less clear, although loss yeast causes Here, we...
ABSTRACT Kinesins are tightly regulated in space and time to control their activation the absence of cargo-binding. Kinesin-binding protein (KIFBP) was recently discovered bind catalytic motor heads 8 45 known kinesin superfamily members inhibit binding microtubules. In humans, mutation KIFBP gives rise Goldberg-Shprintzen syndrome (GOSHS), but kinesin(s) that is misregulated produce clinical features disease not known. Understanding structural mechanism by which selects its partners will be...