A5109286533- Aquaculture disease management and microbiota
- interferon and immune responses
- Parasite Biology and Host Interactions
- Immune Response and Inflammation
- Invertebrate Immune Response Mechanisms
- Helminth infection and control
- Immune Cell Function and Interaction
- Vibrio bacteria research studies
- Fish Biology and Ecology Studies
- Cytokine Signaling Pathways and Interactions
- Viral Infections and Vectors
- Parasites and Host Interactions
- Antimicrobial Peptides and Activities
- Myxozoan Parasites in Aquatic Species
- Aquaculture Nutrition and Growth
- Bacteriophages and microbial interactions
- Cancer-related molecular mechanisms research
- Fish Ecology and Management Studies
- Aquatic Invertebrate Ecology and Behavior
- Chemokine receptors and signaling
- T-cell and B-cell Immunology
- Identification and Quantification in Food
- Microbial infections and disease research
- Marine Bivalve and Aquaculture Studies
- Genetic diversity and population structure
Institute of Hydrobiology
2016-2025
Qingdao Agricultural University
2017-2025
Qingdao National Laboratory for Marine Science and Technology
2017-2025
Lanzhou Jiaotong University
2025
Henan University
2025
Guangxi University of Chinese Medicine
2025
Chinese Academy of Sciences
2015-2024
State Key Laboratory of Freshwater Ecology and Biotechnology
2008-2024
State Key Laboratory of Food Science and Technology
2024
Nanchang University
2024
The DExD/H domain containing RNA helicases such as retinoic acid-inducible gene I (RIG-I) and melanoma differentiation-associated 5 (MDA5) are key cytosolic pattern recognition receptors (PRRs) for detecting nucleotide pathogen associated molecular patterns (PAMPs) of invading viruses. RIG-I MDA5 proteins differentially recognise conserved PAMPs in double stranded or single viral molecules, leading to activation the interferon system vertebrates. They share three core protein domains...
Abstract Background Interferon regulatory factors (IRFs), which can be identified based on a unique helix-turn-helix DNA-binding domain (DBD) are large family of transcription involved in host immune response, haemotopoietic differentiation and immunomodulation. Despite the identification ten IRF members mammals, some recent effort to identify these fish, relatively little is known composition other classes vertebrates, evolution probably origin have not been investigated vertebrates....
The retinoic acid-inducible gene I (RIG-I)-like receptors (RLR) comprise three homologues: RIG-I, melanoma differentiation-associated 5 (MDA5), and laboratory of genetics physiology 2 (LGP2). They activate the host interferon (IFN) system upon recognition viral RNA pathogen-associated molecular patterns (PAMPs) in cytoplasm. Bioinformatic analysis sequenced vertebrate genomes suggests that cytosolic surveillance is conserved lower vertebrates, recent functional studies have confirmed RIG-I...
Further to the previous finding of rainbow trout rtCATH_1 gene, this paper describes three more cathelicidin genes found in salmonids: two Atlantic salmon, named asCATH_1 and asCATH_2, one trout, rtCATH_2. All new salmonid share common characteristics mammalian genes, such as consisting four exons possessing a highly conserved preproregion invariant cysteines clustered C-terminal region cathelin-like domain. The gene is homologous that it possesses repeat motifs TGGGGGTGGC exon IV cysteine...
Abstract Interferons ( IFN s) have been reported in vertebrates from fish to mammals. To date, two types of s, i.e. type I and II s a range species aquaculture. In fish, are clarified into group , with cysteines four cysteines, further subgroups a, d, e, h, b, c, f, respectively. Group appear exist all species, whereas discovered only certain such as cyprinids, salmonids perciform fish. It seems preferentially functional that signal through cytokine receptor family B CRFB ) 5 1, while 2. But...
Abstract For a virus to replicate efficiently, it must try and inhibit host IFN expression because is an important defense at early stages after viral infection. aquatic viruses, the mechanisms used escape hosts system are still unclear. In this study, we show that N protein of spring viremia carp (SVCV) inhibits zebrafish IFNφ1 production by degrading mitochondrial antiviral signaling (MAVS). First, upregulation promoter activity stimulated polyinosinic:polycytidylic acid, retinoic...
Flavobacterium columnare, a member of the phylum Bacteroidetes, causes columnaris disease in wild and aquaculture-reared freshwater fish. The mechanisms responsible for are not known. Many members Bacteroidetes use type IX secretion systems (T9SSs) to secrete enzymes, adhesins, proteins involved gliding motility. F. columnare genome has all genes needed encode T9SS. gldN, which encodes core component T9SS, was deleted wild-type strains ΔgldN mutants were deficient several extracellular...
Abstract Interferons (IFNs) are critical soluble factors in the immune system and composed of three types, (I, II III) that utilize different receptor complexes IFN-αR1/IFN-αR2, IFN-γR1/IFN-γR2, IFN-λR1/IL-10R2, respectively. Here we identify IFN-υ from genomic sequences vertebrates. The members class cytokine receptors, IFN-υR1 IL-10R2, identified as complex IFN-υ, associated with stimulated gene expression antiviral activity zebrafish ( Danio rerio ) African clawed frog Xenopus laevis )....
Type I and III IFNs are structurally related cytokines with similar antiviral functions. They have different genomic organizations bind to distinct receptor complexes. It has been vigorously debated whether the recently identified intron containing IFN genes in fish amphibians belong type or family diverged from a common ancestral gene, that subsequently gave rise both types. In this report, we tandemly linked Xenopus tropicalis genome hence demonstrate for first time relatively early...
IFN regulatory factor (IRF) 10 belongs to the IRF family and exists exclusively in birds fish. Most IRFs have been identified as critical regulators responses both fish mammals; however, role of IRF10 is unclear. In this study, we zebrafish (Danio rerio) found that it serves a negative regulator balance innate antiviral immune responses. Zebrafish (DrIRF10) was induced by intracellular polyinosinic:polycytidylic acid ZF4 (zebrafish embryo fibroblast-like) cells. DrIRF10 inhibited activation...
Cytochrome P450 (Cyp)17A1 has both 17α-hydroxylase and 17,20-lyase activities, which are involved in the steroidogenic pathway that produces androgens estrogens. Previously, a phenotype of all-male cyp17a1-deficient zebrafish generated by transcription activatorlike effector nuclease been reported. In current study, mechanisms relating to Cyp17a1 development sexual traits, especially gonadal differentiation testicular development, were characterized. We found fish at 3 months...
Spring viremia of carp virus (SVCV) is an efficient pathogen causing high mortality in the common carp. Fish interferon (IFN) a powerful cytokine enabling host cells to establish antiviral response; therefore, strategies that SVCV uses avoid cellular IFN response were investigated. Here, we report P protein phosphorylated by TANK-binding kinase 1 (TBK1), which decreases regulatory factor 3 (IRF3) phosphorylation and suppresses production. First, overexpression inhibited promoter activation...
Background Interferon regulatory factor (IRF) 10 was first found in birds and is present the genome of other tetrapods (but not humans mice), as well teleost fish. The functional role IRF10 vertebrate immunity relatively unknown compared to IRF1-9. target this research clone characterize genes three economically important fish species that will facilitate future evaluation molecule innate adaptive immunity. Molecular Characterization Three Fish Species In study, a single gene cloned grass...
Abstract Nucleotide oligomerization domain–like receptors (NLRs) and RIG-I–like (RLRs) detect diverse pathogen-associated molecular patterns to activate the innate immune response. The role of mammalian NLR NOD1 in sensing bacteria is well established. Although several studies suggest also plays a viruses, mechanisms behind this are still largely unknown. In study, we report on synergism antagonism between MDA5 isoforms teleost. zebrafish, overexpression enhances antiviral response mRNA...