A5010996420- Cellular Mechanics and Interactions
- 3D Printing in Biomedical Research
- Cancer Cells and Metastasis
- Monoclonal and Polyclonal Antibodies Research
- Planarian Biology and Electrostimulation
- Collagen: Extraction and Characterization
- Health and Medical Research Impacts
- Biocrusts and Microbial Ecology
- Innovative Microfluidic and Catalytic Techniques Innovation
- Advanced Biosensing Techniques and Applications
- Immunotherapy and Immune Responses
- Diversity and Career in Medicine
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Viral Infections and Immunology Research
- SARS-CoV-2 and COVID-19 Research
- Advances in Oncology and Radiotherapy
Washington University in St. Louis
2022-2025
University of Hong Kong
2024
University of California, Los Angeles
2022
Georgia Institute of Technology
2019-2021
The Wallace H. Coulter Department of Biomedical Engineering
2019-2021
Cells sense and migrate across mechanically dissimilar environments throughout development disease progression. However, it remains unclear whether mechanical memory of past empowers cells to navigate new, three-dimensional extracellular matrices. Here, we show that previously primed on stiff, compared with soft, matrices generate a higher level forces remodel collagen fibers promote invasion. This priming advantage persists in dense or stiffened collagen. We explain this memory-dependent,...
The breast tumor microenvironment is composed of heterogeneous cell populations, including normal epithelial cells, cancer-associated fibroblasts, and cells that lead collective invasion. Both leader CAFs are known to play important roles in invasion across the collagen-rich stromal boundary. However, their individual abilities utilize cell-intrinsic protrusions perform force-based collagen remodeling collectively invade remain unclear. To compare phenotypes leader-like CAFs, we embedded...
During disease and development, physical changes in extracellular matrix cause jamming, unjamming, scattering epithelial migration. However, whether disruptions topology alter collective cell migration speed cell-cell coordination remains unclear. We microfabricated substrates with stumps of defined geometry, density, orientation, which create obstructions for migrating cells. Here, we show that cells lose their directionality when moving through densely spaced obstructions. Although leader...
Abstract Isolating cells based on their secreted proteins remain a challenge. The authors demonstrate capacity for high throughput single‐cell protein secretion analysis and isolation heterofunctional particles combined with fluorescence activated cell sorting (FACS). workflow shows that antibody secreting (ASCs) specific the H1 from influenza virus can be isolated B cells. consists of incubating anti‐CD27 ASCs, capturing locally immunoglobulins Protein G particles, identifying to via...
Bead reagents are used in a large number of assays bioscience and biotechnology to collect purify antibodies by immobilization. Bead-based immunoassays offer high-throughput analysis multiple single sample. Although variety antibody-binding moieties on the collection beads have been studied, physical material properties not optimized isolate specific over broad range concentrations from complex environments containing cells. We present study how optimally use microparticles coated with...
Abstract Cellular forces and intercellular cooperation generate collective cell migration. Pathological changes in cell-level genetic physical properties cause jamming, unjamming, scattering epithelial Separately, microenvironment stiffness confinement can produce varying modes of However, it remains unclear whether how mesoscale disruptions matrix topology alter To address this question, we microfabricated matrices with stumps defined geometry, density, orientation, which serve as...
Abstract Clinical research output in the emergency department (ED) continues to be constrained by limitations funding for researchers, demands of patient care on ED providers, and difficulties obtaining high‐quality data. In response, several institutions have established programs which student volunteers are integrated into workflows increase clinical introduce pre‐health students careers medicine. One such program, volunteer presently consists over 40 undergraduate post‐baccalaureate who...
Abstract In disease and development, cells sense migrate across mechanically dissimilar environments. We investigated whether mechanical memory of past environments empowers to navigate new, three-dimensional Here, we show that primed by stiff matrices apply higher forces, compared soft-primed cells, accumulate align collagen fibers towards sustained invasion. This priming advantage persists in dense or stiffened collagen. Through an energy-minimization model, elucidate how memory-laden...