A5063022734- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- DNA and Nucleic Acid Chemistry
- MicroRNA in disease regulation
- Bone Tissue Engineering Materials
- Osteoarthritis Treatment and Mechanisms
- Electrospun Nanofibers in Biomedical Applications
- Dental Implant Techniques and Outcomes
- Bacteriophages and microbial interactions
- Graphene and Nanomaterials Applications
- Angiogenesis and VEGF in Cancer
- Advanced Nanomaterials in Catalysis
- Periodontal Regeneration and Treatments
- Biosensors and Analytical Detection
- HIV Research and Treatment
- Antimicrobial Peptides and Activities
- Immunotherapy and Immune Responses
- Nanopore and Nanochannel Transport Studies
- Nanoplatforms for cancer theranostics
- HER2/EGFR in Cancer Research
- Mesenchymal stem cell research
- Nanoparticle-Based Drug Delivery
- Nuclear Structure and Function
- Breast Cancer Treatment Studies
- Reconstructive Surgery and Microvascular Techniques
State Key Laboratory of Oral Diseases
2016-2025
Stomatology Hospital
2017-2025
Sichuan University
2016-2025
West China Hospital of Sichuan University
2022-2024
Yale University
2020-2023
Peking University
2019
Peking University Cancer Hospital
2019
Chengdu University
2017-2018
Gouty arthritis is a very familiar inflammatory arthritis. Controlling inflammation the key to preventing gouty However, colchicine, most highly represented drug used in clinical practice, has strict contraindications owing some severe side effects. Curcumin (Cur), natural anti-inflammatory drug, demonstrated good safety and efficacy. rapid degradation, poor aqueous solubility, low bioavailability of Cur limit its therapeutic effect. To strengthen effectiveness Cur. loaded tetrahedral...
Abstract Rapid and efficient tools for early cancer detection have diagnostic therapeutic value. Given that the DNA hairpin‐based hybridization chain reaction (HCR) is effective in detecting various biological targets, a tetrahedral framework DNA‐enhanced (TDN‐enhanced) HCR system (T‐probe system) introduced cancer‐related targets its versatility demonstrated by intracellular target miRNA 21 cellular membrane nucleolin. Benefiting from spatial confinement of TDN, T‐probe demonstrates high...
Tetrahedral framework nucleic acids (tFNAs) have attracted extensive attention as drug nanocarriers because of their excellent cellular uptake. However, for oligonucleotide cargos, tFNA mainly acts a static delivery platform generated via sticky-ended ligation. Here, inspired by the original stable space inside tetrahedral scaffold, dynamic lysosome-activated nanobox is fabricated completely encapsulating short interfering RNA (siRNA) interest. The closed structure endows cargo siRNA with...
MicroRNA (miR)-based therapy shows strong potential; however, structural limitations pose a challenge in fully exploiting its biomedical functionality. Tetrahedral framework DNA (tFNA) has proven to be an ideal vehicle for miR therapy. Inspired by the ancient Chinese myth "Sun and Immortal Birds," novel bioswitchable inhibitor delivery system (BiRDS) is designed with three inhibitors (the immortal birds) nucleic acid core central sun). The BiRDS fuses within framework, maximizing their...
Abstract MicroRNAs (miRs) play an important role in regulating gene expression. Limited by their instabilities, miR therapeutics require delivery vehicles. Tetrahedral framework nucleic acids (tFNAs) are potentially applicable to drug because they prominently penetrate tissue and taken up cells. However, tFNA‐based strategies have failed separate the miRs after enter cells, affecting efficiency. In this study, RNase H‐responsive sequence is applied connect a sticky‐end tFNA (stFNA) miR‐2861,...
Abstract Systemic immune monitoring is a crucial clinical tool for disease early diagnosis, prognosis and treatment planning by quantitative analysis of cells. However, conventional using flow cytometry faces huge challenges in large-scale sample testing, especially mass health screenings, because time-consuming, technical-sensitive high-cost features. the lack high-performance detection platforms hinders development high-throughput technology. To address this bottleneck, we constructed...
Tetrahedral DNA nanostructures (TDNs) are a new type of nanomaterials that have recently attracted attention in the field biomedicine. However, practical application is often limited owing to host immune response. Here, response RAW264.7 macrophages TDNs was comprehensively evaluated. The results showed had no observable cytotoxicity and could induce polarization cells M1 type. attenuated expression NO IL-1β (interleukin-1β), IL-6 (interleukin-6), TNF-α (tumor necrosis factor-α) LPS-induced...
Cartilage tissue engineering based on biomimetic scaffolds has become a rapidly developing strategy for repairing cartilage defects. In this study, biphasic CAN-PAC hydrogel osteochondral defect (OCD) regeneration was fabricated the density difference between two layers via thermally reactive, rapid cross-linking method. The upper cross-linked by CSMA and NIPAm, lower composed of PECDA, AAm PEGDA. interface first grafted physical calcium gluconate alginate, followed chemical carbon-carbon...
In recent years, there has been a rapid progress in the development of DNA nanomaterials for biological applications. Owing to their unsurpassed editability, various with well-documented biocompatibility and relative stability have developed. Among these tetrahedral nanostructures (TDNs), as typical multi-arm tiles 3D polyhedral, is major research focus owing structural features. TDNs yielded satisfactory results regulators, biosensors, targeted delivery vehicles. Extensive studies on...
Bone tissue engineering emerges as an advantageous technique to achieve regeneration. Its scaffolds must present excellent biomechanical properties, where bare polymers poorly perform. Development of new biomaterials with high osteogenic capacity is urgently pursued. In this study, electrospun poly(3-hydroxybutyrate-co-4-hydroxybutyrate)/graphene oxide (P34HB/GO) nanofibrous scaffold successfully fabricated and characterized. The effects GO amount on morphology, cellular behaviors are...
Periodontitis is a common disease that causes periodontium defects and tooth loss. Controlling inflammation tissue regeneration are two key strategies in the treatment of periodontitis. Tetrahedral framework nucleic acids can modulate multiple biological behaviors, thus, their applications have been widely explored. In this study, we investigated effect tFNAs on under inflammatory conditions. Lipopolysaccharide silk ligature were used to induce vivo vitro. The results displayed decreased...
Abstract Osteoarthritis, a disorder characterized by articular cartilage deterioration, varying degrees of inflammation, and chondrocyte apoptosis, is the most common chronic joint disease. To slow or reverse its progression, inflammation should be inhibited, proliferation promoted. Tetrahedral framework nucleic acids can internalized chondrocytes (even inflammatory chondrocytes) enhance their migration. Wogonin, naturally occurring flavonoid, suppresses oxidative stress inhibits...
Abstract DNA nanotechnology has provided new strategies and platforms for cargo delivery. However, DNA‐based nanostructures utilized delivery have mainly focused on static structures passive targeting. Here, inspired by honeybee's natural reaction to hazards, a melittin loaded tetrahedron framework nucleic acid (tFNA) nanostructure, active targeting therapy as “nanobee” is developed. Upon exposure target proteins the cell membrane, tFNA exoskeleton goes through conformation change, leading...
siRNA is found to effectively knock down the target gene in cells, which considered a promising strategy for therapy. However, application of limited due its low efficiency cellular uptake. Tetrahedral framework nucleic acids (tFNAs) are synthesized by four single-stranded DNAs and show multiple biological functions recent studies, especially suitable drug delivery. More than 60% malignant melanomas associated with Braf mutation, an attractive therapeutic RNA interference. In this study, we...
Erythromycin is a commonly used broad-spectrum antibiotic, but resistance to this antibiotic makes its use less effective. Considerable efforts, beside finding alternatives, are needed enhance antimicrobial effect and stability against bacteria. Tetrahedral framework nucleic acids (tFNAs), novel delivery vehicle with three-dimensional nanostructure, have been studied as carrying platform of antineoplastic drugs. In study, the tFNAs in delivering erythromycin into
Increasing evidence has suggested that the HIV-1 capsid enters nucleus in a largely assembled, intact form. However, not much is known about how cone-shaped interacts with nucleoporins (NUPs) nuclear pore for crossing complex. Here, we elucidate NUP153 binds by engaging assembled protein (CA) lattice. A bipartite motif containing both canonical and noncanonical interaction modules was identified at C-terminal tail region of NUP153. The cargo-targeting phenylalanine-glycine (FG) engaged CA...