A5071526749- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- DNA and Nucleic Acid Chemistry
- Bacteriophages and microbial interactions
- RNA and protein synthesis mechanisms
- CRISPR and Genetic Engineering
- DNA and Biological Computing
- Lipid Membrane Structure and Behavior
- Modular Robots and Swarm Intelligence
- Medical Imaging and Analysis
- Radiomics and Machine Learning in Medical Imaging
- RNA modifications and cancer
- Molecular Junctions and Nanostructures
- Bacterial Genetics and Biotechnology
- Biosensors and Analytical Detection
- Nanocluster Synthesis and Applications
- Molecular Sensors and Ion Detection
- Antimicrobial Peptides and Activities
- MRI in cancer diagnosis
- Advanced Nanomaterials in Catalysis
- Biochemical and Structural Characterization
- Nanowire Synthesis and Applications
- Inorganic Chemistry and Materials
- Nanopore and Nanochannel Transport Studies
- Surfactants and Colloidal Systems
Fudan University Shanghai Cancer Center
2017-2025
State Key Laboratory of Genetic Engineering
2025
Shanghai Jiao Tong University
2019-2025
Fudan University
2018-2024
Shanghai Stomatological Hospital
2020-2024
Shanghai Xuhui Central Hospital
2023-2024
Nanjing Maternity and Child Health Care Hospital
2024
Nanjing Medical University
2024
Pudong Medical Center
2020-2023
Tongji University
2023
The blooming field of structural DNA nanotechnology harnessing the material properties nucleic acids has attracted widespread interest. exploitation precise and programmable Watson–Crick base pairing or RNA led to development exquisite acid nanostructures from one three dimensions. advances computer-aided tools facilitate automated design with various sizes shapes. Especially, construction shell skeleton frameworks, more recently dubbed "framework acids" (FNAs) provides a means organize...
Abstract The proper regulation of transcription is essential for maintaining genome integrity and executing other downstream cellular functions 1,2 . Here we identify a stable association between the genome-stability regulator sensor single-stranded DNA (SOSS) 3 Integrator-PP2A (INTAC) 4–6 Through SSB1-mediated recognition DNA, SOSS–INTAC stimulates promoter-proximal termination attenuates R-loops associated with paused RNA polymerase II to prevent R-loop-induced instability....
DNA phosphoester bonds are exceedingly resistant to hydrolysis in the absence of chemical or enzymatic catalysts. This property is particularly important for organisms with large genomes, as resistance hydrolytic degradation permits long-term storage genetic information. Here we report creation and analysis two classes engineered deoxyribozymes that selectively rapidly hydrolyze DNA. Members class I carry a catalytic core composed only 15 conserved nucleotides attain an observed rate...
Abstract Platinum (Pt) resistance in cancer almost inevitably occurs during clinical Pt-based chemotherapy. The spontaneous nucleotide-excision repair of cells is a representative process that leads to Pt resistance, which involves the local DNA bending facilitate recruitment proteins and subsequent elimination Pt-DNA adducts. By exploiting structural vulnerability this process, we herein report nuclease-mimetic nanozyme can target cell nuclei induce concurrent platination oxidative cleavage...
Energy metabolism is highly interdependent with adaptive cell migration in vivo. Mechanical confinement a critical physical cue that induces switchable modes of the mesenchymal-to-amoeboid transition (MAT). However, energy states distinct modes, especially amoeboid-like stable bleb (A2) movement, remain unclear. In this report, we developed multivalent DNA framework-based nanomachines to explore strategical mitochondrial trafficking and differential ATP levels during mechanically...
The construction of DNA origami nanostructures is heavily dependent on the folding scaffold strand, which typically a single-stranded genome extracted from bacteriophage (M13). Custom scaffolds can be prepared in number methods, but they are not widely accessible to broad user base nanotechnology community. Here, we explored new design and possibilities with custom our cost- time-efficient production pipeline. According pipeline, de novo produced variety specified local global sequence...
Abstract Titanium (Ti) is extensively used in the medical field because of its excellent biomechanical properties; however, how to precisely fabricate Ti surfaces at a nanoscale remains challenging. In this study, DNA nanocoating system functionalize via series sequential reactions involving hydroxylation, silanization, and click chemistry developed. Tetrahedral nanostructures (TDNs) two different sizes (≈7 30 nm) are assembled characterized for subsequent surface attachment. vitro vivo...
MutS is a DNA repair protein that recognizes unpaired and bulged bases. When it binds to DNA, bends the double helix. We have developed novel DNA-based nanomechanical device measures amount of work DNA-bending can do when The we report here scissors-like consisting two double-crossover (DX) molecules connected each other by flexible Holliday junction. DX components are helix contains binding site for MutS; duplex bent, scissors contracts. also joined sticky ends on an edge adjacent site; be...
Riboswitches for the bacterial second messenger c-di-GMP control expression of genes involved in numerous cellular processes such as virulence, competence, biofilm formation, and flagella synthesis. Therefore, two known riboswitch classes represent promising targets developing novel modulators physiology. Here, we examine binding characteristics circular linear analogues representatives both class I II riboswitches derived from pathogenic bacterium Vibrio choleae (class I) Clostridium...
A series of allosteric ribozymes that respond to the bacterial second messenger cyclic diguanosyl-5'-monophosphate (c-di-GMP) have been created by using in vitro selection. An RNA library was generated random-sequence bridges join a hammerhead self-cleaving ribozyme an aptamer from natural c-di-GMP riboswitch. Specific bridge sequences, called communication modules, emerged through two selection efforts either activate or inhibit self-cleavage upon ligand binding aptamer. Representative RNAs...
The excellent programmability and modifiability of DNA has enabled chemists to reproduce a series specific molecular interactions in self-assembled synthetic systems. Among diverse modifications, cholesterol conjugation can turn into an amphiphilic molecule (cholesterol-DNA), driving the formation assemblies through cholesterol-endowed hydrophobic interaction. However, precise control such assembly process remains difficult because unbiased accumulation cholesterol. Here, we report...
Specific DNA-binding to metal ions is a long-standing fundamental research topic with great potential transform into nano/biotechnology and therapeutics applications. Herein, based on the mobility change of DNA in denaturing gels, we develop selection strategy discover series 40–45 nt small DNAs that can bind Zn2+ Cd2+ specifically tightly. The Zn2+- Cd2+-bound complexes even tolerate harsh conditions 8 M urea 50 mM EDTA. discovery not only exposes new class transition ion-binding but also...
A prototype of DNA nanorobot with the ability to transport molecular payloads was designed target cancer cells in tissue culture. Moreover, a further step taken succeed first vivo application for therapy. The robot constructed using aptamer and origami fold 90-nm tubular device carry blood coagulation protease thrombin inside, shielded from circulating platelets plasma fibrinogen. recognition binding its tumour-specific molecule triggered unfolding expose blood, which turn activated at local...
DNA molecules that encode a small, high-speed self-hydrolyzing deoxyribozyme are used as templates for rolling circle amplification (RCA) to produce single-stranded DNAs (ssDNAs) of single- and multiple-unit lengths. Including self-cleaving deoxyribozymes in RCA products can generate large amounts ssDNAs with defined sequence length well precise termini. We also demonstrate the use this method efficiently ssDNA size markers by using reaction conditions permit partial processing.