A5022252979- Infectious Disease Case Reports and Treatments
- Bacterial Infections and Vaccines
- Infections and bacterial resistance
- Biliary and Gastrointestinal Fistulas
- Genomics and Phylogenetic Studies
- Pneumonia and Respiratory Infections
- Bacterial Genetics and Biotechnology
- Influenza Virus Research Studies
- Bacterial Identification and Susceptibility Testing
- Infective Endocarditis Diagnosis and Management
- Antibiotic Resistance in Bacteria
- Infectious Encephalopathies and Encephalitis
- Bacteriophages and microbial interactions
- Streptococcal Infections and Treatments
- Nutrition, Genetics, and Disease
- RNA and protein synthesis mechanisms
- Legionella and Acanthamoeba research
- Advanced biosensing and bioanalysis techniques
- Genetics, Bioinformatics, and Biomedical Research
- Pediatric Urology and Nephrology Studies
University of Bath
2019-2022
Applied Sciences (United States)
2020
Roslin Institute
2014-2017
University of Edinburgh
2010-2017
University of Guelph
2007-2011
Centre for Immunity, Infection and Evolution
2010
Irish Equine Centre
2010
We report the genome of facultative intracellular parasite Rhodococcus equi, only animal pathogen within biotechnologically important actinobacterial genus Rhodococcus. The 5.0-Mb R. equi 103S is significantly smaller than those environmental rhodococci. This due to expansion in nonpathogenic species, via a linear gain paralogous genes and an accelerated genetic flux, rather reductive evolution equi. lacks extensive catabolic secondary metabolic complement rhodococci, it displays unique...
We report a novel host-associated virulence plasmid in Rhodococcus equi, pVAPN, carried by bovine isolates of this facultative intracellular pathogenic actinomycete. Surprisingly, pVAPN is 120-kb invertron-like linear replicon unrelated to the circular plasmids associated with equine (pVAPA) and porcine (pVAPB variant) R. equi isolates. similar pNSL1 from sp. NS1 harbors six new vap multigene family members (vapN vapS) pathogenicity locus presumably acquired via en bloc mobilization direct...
ObjectivesThe objective of this study was to identify the molecular mechanism macrolide resistance in actinomycete Rhodococcus equi, a major equine pathogen and zoonotic agent causing opportunistic infections people.
The conjugative virulence plasmid is a key component of the Rhodococcus equi accessory genome essential for pathogenesis. Three host-associated types have been identified equine pVAPA and porcine pVAPB circular variants, linear pVAPN found in bovine (ruminant) isolates. We recently characterized R. pangenome (Anastasi E, et al. 2016. Pangenome phylogenomic analysis pathogenic actinobacterium equi. Genome Biol Evol. 8:3140-3148.) we report here comparative genomes. Plasmids within each type...
ABSTRACT PagL and LpxO are enzymes that modify lipid A. is a 3-O deacylase removes the primary acyl chain from 3 position, an oxygenase 2-hydroxylates specific chains in pagL lpxO homologues have been identified genome of Bordetella bronchiseptica , but current structure for B. A position acylated 2-OH acylation not reported. We investigated role biosynthesis. report different wild-type (WT) A, including presence 2-OH-myristate, which dependent on . also demonstrate major WT structures...
The identification of genes essential for a bacterium's growth reveals much about its basic physiology under different conditions. Bordetella pertussis, the causative agent whooping cough, adopts both virulent and avirulent states through activity two-component system, Bvg. B. pertussis in vitro were defined using transposon sequencing, Bvg-determined states. In addition, comparison insertion indices each gene between Bvg phases identified those whose mutation exerted significantly fitness...
The evolution of Bordetella pertussis and parapertussis from bronchiseptica involved changes in host range pathogenicity. Recent data suggest that the human-adapted modified their interaction with immune systems to effect these decreased stimulation Toll-like receptor 4 (TLR4) by lipid A is central this. We discuss structure genetics within context immunity.
Whooping cough, or pertussis, is resurgent in numerous countries worldwide. This has renewed interest Bordetella pertussis biology and vaccinology. The vitro growth of B. been a source difficulty, both for the study organism production vaccines. It inhibited by fatty acids other hydrophobic molecules. AcrAB efflux system present many different bacteria combination with an outer membrane factor exports acriflavine small molecules from cell. Here, we identify that speciation selected Acr...
Bacterial genetic diversity is often described solely using base-pair changes despite a wide variety of other mutation types likely being major contributors. Tandem duplication/amplifications are thought to be widespread among bacteria but due their often-intractable size and instability, comprehensive studies these mutations rare. We define methodology investigate amplifications in bacterial genomes based on read depth genome sequence data as proxy for copy number. demonstrate the approach...
Bordetella pertussis is the causative agent of whooping cough, commonly referred to as pertussis. Although incidence was reduced through vaccination, during last thirty years it has returned high levels in a number countries. This resurgence been linked switch from use whole-cell acellular vaccines. Protection afforded by vaccines appears be short-lived compared that whole cell In order inform future vaccine improvement identifying immune correlates protection, human challenge model B....
Abstract Despite effective vaccines to protect against Whooping cough, or pertussis, the disease is resurgent in many countries. A switch from acellular whole-cell has resulted waning protective immunity, likely contributing increases infection prevalence, underlining need better understand B. pertussis virulence. As a respiratory pathogen, colonises upper tract utilising an array of adhesins, four which (FHA, pertactin, Fim2/3) are included vaccine. In this study, we identified two Bvg...
Abstract Bacterial genetic diversity is often described using solely base pair changes despite a wide variety of other mutation types likely being major contributors. Tandem duplications genomic loci are thought to be widespread among bacteria but due their intractable size and instability, comprehensive studies the range genome dynamics these mutations rare. We define methodology investigate in bacterial genomes based on read depth sequence data as proxy for copy number. demonstrate...
Abstract Bordetella pertussis is the causative agent of whooping cough, commonly referred to as pertussis. Although incidence was reduced through vaccination, during last thirty years it has returned high levels in a number countries. This resurgence been linked switch from use whole-cell acellular vaccines. Protection afforded by vaccines appears be short-lived compared that whole cell In order inform future vaccine improvement identifying immune correlates protection, human challenge model...
This protocol describes the use of qPCR for quantitative determination live B. pertussis bacteria in a sample. The method uses propidium monoazide, compound that can cross cell wall dead and binds DNA following photo activation to inhibit amplification from bacteria. TaqMan probe specific IS481 ensure maximum sensitivity due high copy number target. Although is not experimental purposes it provides acceptable specificity.