A5061464115- Animal Disease Management and Epidemiology
- Bacterial Identification and Susceptibility Testing
- Microbial infections and disease research
- Brucella: diagnosis, epidemiology, treatment
- Salmonella and Campylobacter epidemiology
- Streptococcal Infections and Treatments
- SARS-CoV-2 detection and testing
- SARS-CoV-2 and COVID-19 Research
- Animal Behavior and Welfare Studies
- Zoonotic diseases and public health
- Antimicrobial Resistance in Staphylococcus
- Burkholderia infections and melioidosis
- Infective Endocarditis Diagnosis and Management
- Viral gastroenteritis research and epidemiology
- Animal Diversity and Health Studies
- Reproductive tract infections research
- Antibiotic Resistance in Bacteria
- Mycobacterium research and diagnosis
- Viral Infections and Vectors
- Animal Virus Infections Studies
- Biosensors and Analytical Detection
- Vector-borne infectious diseases
- Leptospirosis research and findings
- Bacteriophages and microbial interactions
- Clostridium difficile and Clostridium perfringens research
Universidad Complutense de Madrid
2016-2025
Fundación Ramón Domínguez
2018
Universidad Politécnica de Madrid
2015-2017
Since March 2020, Spain (along with many other countries) has been severely affected by the ongoing coronavirus disease 19 (COVID-19) pandemic caused rapid spread of a new virus (severe acute respiratory syndrome 2; SARS-CoV-2). As part global efforts to improve surveillance, we investigated how readily SARS-CoV-2 RNA could be detected in environmental samples collected from an isolated rural community high COVID-19 prevalence (6% population 883 inhabitants). The first diagnosis...
The disease produced by the severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) is currently one of primary concerns worldwide. Knowing zoonotic origin and that several animal species, including dogs cats, are susceptible to viral infection, it critical assess relevance pets in this pandemic. Here, we performed a large-scale study on SARS-CoV-2 serological prevalence cats Spain order elucidate their role susceptibility. Samples from animals contact with COVID-19 positive...
The emergence of the Omicron variant (B.1. 1.529) has brought with it an increase in incidence SARS-CoV-2 disease. However, there is hardly any data on its companion animals. We have detected presence this new domestic animals (dogs and cats) living infected owners Spain. None RT-qPCR positive (10.13%) presented clinical signs viral loads were low. In addition, shedding RNA lasted a short period time Infection concern (VOC) was confirmed by sequencing. These outcomes suggest lower virulence...
Lactococci have been associated with fetal and neonatal infections in humans cattle. Here we describe a case of reproductive loss attributed to Lactococcus petauri lemur. A full-term black-and-white ruffed lemur (Varecia variegata) was found dead the indoor area zoologic exhibit. Classification as late-term abortion or stillbirth unclear precise gestational time unknown. medical checkup dam revealed fever neutrophilic leukocytosis; recovery followed treatment enrofloxacin. The main...
Background/Objectives: Antimicrobial resistance (AMR) poses a significant threat to global health and the economy, with projected costs ranging from $300 billion $1 trillion annually an estimated 10 million deaths per year by 2050. The food chain, primary production retail, represents critical entry point for antimicrobial resistant bacteria into communities. This underscores need coordinated “One Health” approach, integrating efforts in animal production, environmental health, human...
Brucellosis is endemic in most parts of Egypt, where it caused mainly by Brucella melitensis biovar 3, and affects cattle small ruminants spite ongoing efforts devoted to its control. Knowledge the predominant species/strains circulating a region prerequisite brucellosis control strategy. For this reason study aiming at evaluation phenotypic genetic heterogeneity panel 17 spp. isolates recovered from domestic (cattle, buffalo, sheep, goat) four governorates during period five years...
Leishmaniasis is one of the most important neglected zoonosis and remains endemic in at least 88 developing countries world. In addition, anthropogenic environmental changes urban areas are leading to its emergency world wide. Zoonotic leishmaniasis control might only be achieved by an integrated approach targeting both human host animal reservoirs, which certain sylvatic cycles yet identified. Recently, hares have been pointed out as competent reservoirs Leishmania infantum Spain, but role...
Abstract The aim of this study was to evaluate the usefulness MALDI‐TOF MS identify 151 isolates Aeromonas obtained mostly from diseased fish. correctly identified all genus level but important differences in percentage depending on species were observed. Considering exclusively first identification option, bestiarum, hydrophila, salmonicida, veronii and sobria best with results >95%. However, considering second options, only that showed values >90% A. hydrophila . Overall, when...
Natural and experimental SARS-CoV-2 infection in pets has been widely evidenced since the beginning of COVID-19 pandemic. Among numerous affected animals, cats are one most susceptible species. However, little is known about viral pathogenicity transmissibility case variants concern (VOCs) animal hosts, such as B.1.617.2 (Delta) variant first detected India. Here, we have identified VOC a cat living with positive owner. The presented mild symptoms (sneezing) high load was oropharyngeal swab,...
Non-tuberculous mycobacteria (NTM) are difficult to identify by biochemical and genetic methods due their microbiological properties complex taxonomy. The development of more efficient rapid for species identification in the veterinary laboratory is, therefore, great importance. Although MALDI-TOF Mass Spectrometry (MS) has become a promising tool NTM human clinical practise, information regarding its performance on isolates is scarce. This study assesses capacity MS (n = 75) obtained from...
The accuracy of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) for identifying Streptococcus suis isolates obtained from pigs, wild animals, and humans was evaluated using a PCR-based identification assay as the gold standard. In addition, MALDI-TOF MS compared with commercial multi-tests Rapid ID 32 STREP system. From 129 S. included in study identified by molecular method, only 31 (24.03%) had score values ≥2.300 79 (61.24%) gave between 2.299...
The application of MALDI-TOF MS for identifying streptococcal isolates recovered from clinical specimens diseased pigs was evaluated. For this proposal, the MALDI BDAL Database (Bruker Daltoniks, Germany) supplemented with main spectrum profiles (MSP) reference strains S. porci, porcorum and plurextorum associated pneumonia septicemia. Although these three species showed similar profiles, several peaks were recognized that can be useful their differentiation: porci (4113, 6133, 7975 8228 m/z...
Trained immunity is the capacity of innate immune cells to produce an improved response against a secondary infection after previous unrelated infection. Salmonellosis represents public health issue and affects pig farming industry. In general, vaccination salmonellosis still facing problems regarding control distinct serovars. Therefore, we hypothesized that immunostimulant based on heat inactivated Mycobacterium bovis (HIMB) could have training effect in pigs challenged with Salmonella...
Staphylococcus aureus encompasses 2 subspecies ( and anaerobius) with significant differences in their epidemiology pathogenicity. We evaluated the suitability of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for rapid identification both using a panel 52 S. isolates (30 subsp. anaerobius 22 aureus) recovered from different origins, countries, years. The on-board library system correctly identified 42 (81%) at species level score values >2.0....
Biochemical and molecular genetic studies were performed on two novel Gram-stain-positive, catalase-negative, coccus-shaped organisms isolated from liquid joint samples of pigs. The micro-organisms not identified as members a recognized species based results cellular, morphological biochemical tests. 16S rRNA gene sequence comparison allowed their identification the genus Jeotgalibaca , but different to dankookensis single genus. shared 96.3 96.9 % similarity values with nearest phylogenetic...
Ovine enzootic abortion, caused by Chlamydia abortus, leads to important economic losses worldwide. In addition reproductive failures, infection may impact lamb growth during the first weeks after birth, yet this effect has not been well characterized. Vaccination can help control disease but variable efficacy values have described, possibly related with factors associated host, vaccine, parameter used for determination, and challenge conditions. context, we evaluated of an inactivated...
Samples from the mesenteric lymph nodes (MS LNs) and ileocecal valves (ICV) of 105 goats, comprising 61 non-vaccinated 44 vaccinated against