A5062005078- Reproductive tract infections research
- Reproductive System and Pregnancy
- Reproductive Physiology in Livestock
- Plant Pathogens and Fungal Diseases
- Cervical Cancer and HPV Research
- Nematode management and characterization studies
- Microbial infections and disease research
- Studies on Chitinases and Chitosanases
- Nanocomposite Films for Food Packaging
- Diphtheria, Corynebacterium, and Tetanus
- Clostridium difficile and Clostridium perfringens research
- Plant and fungal interactions
- Reproductive Health and Contraception
- Genital Health and Disease
- Urinary Tract Infections Management
- Entomopathogenic Microorganisms in Pest Control
- Mycobacterium research and diagnosis
- Rabies epidemiology and control
- Brucella: diagnosis, epidemiology, treatment
- Toxoplasma gondii Research Studies
- Herpesvirus Infections and Treatments
- Helminth infection and control
- Vector-borne infectious diseases
- Mycotoxins in Agriculture and Food
- Mycorrhizal Fungi and Plant Interactions
Universidad de Murcia
2015-2024
Catholic University of Santa María
2021
Hospital Nacional Cayetano Heredia
2021
University of Alicante
2002-2010
Servicio Gallego de Salud
2007
University of Basrah
2005
Universidad de León
2004
Infectiologie Animale et Santé Publique
1995
Souriau (France)
1994
University of Córdoba
1993
Summary The antifungal mode of action chitosan has been studied for the last 30 years, but is still little understood. We have found that plasma membrane forms a barrier to in chitosan‐resistant not chitosan‐sensitive fungi. membranes fungi were shown more polyunsaturated fatty acids than fungi, suggesting their permeabilization by may be dependent on fluidity. A acid desaturase mutant Neurospora crassa with reduced fluidity exhibited increased resistance chitosan. Steady‐state fluorescence...
Aims: To investigate the toxic effect of chitosan on important root pathogenic and biocontrol fungi (nematophagous, entomopathogenic mycoparasitic). Methods Results: We have used standard bioassays to colony growth developed test spore germination. The results showed that mycoparasitic tested were more sensitive than nematophagous fungi. Chitosanases (and perhaps related enzymes) are involved in resistance chitosan. Two fungi, one chitosan, Fusarium oxysporum f. sp. radicis‐lycopersici, less...
Abstract Neutrophil migration to the site of infection is a critical early step in host immunity microbial pathogens, which chemokines and their receptors play an important role. In this work, mice deficient expression chemokine receptor CXCR2 were infected with Toxoplasma gondii outcome was monitored. Gene-deleted animals displayed completely defective neutrophil recruitment, apparent at 4 h sustained for least 36 h. KitW/KitW-v also polymorphonuclear leukocyte migration, suggesting mast...
Surveys (in 2002 and 2003) were performed for fungal endophytes in roots of 24 plant species growing at 12 sites (coastal inland soils, both sandy soils salt marshes) under either water or stress the Alicante province (Southeast Spain). All examined colonized by endophytic fungi. A total 1830 isolates obtained identified morphological molecular [internal transcribed spacer (ITS) translation elongation factor-1alpha gene region (TEF-1alpha) sequencing] techniques. One hundred forty-two...
Abstract The entomopathogenic fungi Beauveria bassiana , Lecanicillium dimorphum and L . cf. psalliotae can survive colonize living palm tissue as endophytes. molecular interaction between these biocontrol agent the date Phoenix dactylifera L. was investigated using proteomic techniques. Field palms inoculated with were analyzed 15 30 days after inoculation in two independent bioassays. In vitro also B or Qualitative quantitative differences protein accumulation controls (not inoculated)...
Wildlife and notably deer species – due to the increasing relevance of farming worldwide may contribute maintenance Coxiella burnetii, causal agent Q fever. Currently, there are no precedents linking exposure with human fever cases. However, a case was recently diagnosed in red (Cervus elaphus) farm, which led us investigate whether could be source for environmental contamination C. burnetii ascertain implication reproductive failure farm. Blood serum vaginal swabs were collected from hinds...
Identifying the aetiological agent of atypical pneumonia in human can sometimes be a tedious process, especially cases where Mycoplasma pneumoniae, Legionella species and Chlamydia pneumoniae are ruled out. In such cases, correct anamnesis patient is basic to clarify which pathogens might have produced infection. For this reason, health professionals including veterinarians laboratory personnel working with zoonotic should keep their doctors informed.A case linked abortus reported. A...
The role of polymorphonuclear neutrophils (PMNs) in the development specific immune response against Chlamydophila abortus (Chlamydia psittaci serotype 1) infection was studied a pregnant mouse model involving treatment with RB6-8C5 monoclonal antibody. PMN depletion significantly affected liver, which T-lymphocyte and F4/80(+) cell populations decreased, particularly CD8(+) T-cell population. A Th1-like response, characterized by high levels gamma interferon without detectable interleukin 4...
This work was aimed at optimizing a protein extraction procedure for date palm (Phoenix dactylifera L.) leaves, highly recalcitrant plant tissue 2-DE. Five protocols based on different precipitation agents (TCA/acetone vs. phenol (Ph) methods) and resolubilization methods (physical treatments, e.g., sonication, shaking and/or heating) were tested. Ph/SDS with methanol/ammonium acetate precipitation, followed by DOC preincubation TCA/acetone and, finally, solubilization in rehydration...
ABSTRACT To assess the role of polymorphonuclear neutrophils (PMNs) in Chlamydia psittaci infection a pregnant mouse model, and nonpregnant Swiss OF1 mice were depleted PMNs by treatment with RB6-8C5 monoclonal antibody before intraperitoneal C. serotype 1. Nondepleted served as controls. Depleted aborted earlier had much higher mortality rate than nondepleted mice. Bacteriological analysis showed that number chlamydiae isolated from spleens at 5 7 days postinfection was 100 times greater...
A Chlamydophila abortus-induced abortion model was carried out on the basis of experimental infection ewes at day 75 gestation. The induced abortions and birth weak lambs during last 3 weeks pregnancy. To study kinetics in placenta other organs, infected were killed 105, 120, 130 days gestation also several after or parturition. Infected developed a systemic that caused mild transient pneumonia focal hepatitis. Pathologic changes observed placentas 120 gestation, although lesions varied...