A5005185558- Escherichia coli research studies
- Viral gastroenteritis research and epidemiology
- Antimicrobial Resistance in Staphylococcus
- Antibiotic Resistance in Bacteria
- Clostridium difficile and Clostridium perfringens research
- Bacterial Genetics and Biotechnology
- Vector-Borne Animal Diseases
- Animal Disease Management and Epidemiology
- Bacterial Identification and Susceptibility Testing
- Salmonella and Campylobacter epidemiology
- Bacterial biofilms and quorum sensing
- Research on Leishmaniasis Studies
- Veterinary medicine and infectious diseases
- Probiotics and Fermented Foods
- Parasitic Infections and Diagnostics
- Pluripotent Stem Cells Research
- T-cell and Retrovirus Studies
- Trypanosoma species research and implications
- Microbial Metabolism and Applications
- Pharmaceutical and Antibiotic Environmental Impacts
- Microbial infections and disease research
- Antibiotics Pharmacokinetics and Efficacy
- Spanish Literature and Culture Studies
- Enterobacteriaceae and Cronobacter Research
- Streptococcal Infections and Treatments
Universidad Complutense de Madrid
2012-2023
Fundación Ramón Domínguez
2018
Pontificia Universidad Católica de Chile
2013
University of California, San Francisco
2006-2007
Universidad de Extremadura
1991-1995
Gabriel René Moreno Autonomous University
1992
Complejo Hospitalario de Cáceres
1991
Technical University of Munich
1987
Calcium-activated chloride channels (CaCCs) are widely expressed in mammalian tissues, including intestinal epithelia, where they facilitate fluid secretion. Potent, selective CaCC inhibitors have not been available. We established a high-throughput screen for identification of human based on inhibition ATP/carbachol-stimulated iodide influx HT-29 cells after lentiviral infection with the yellow fluorescent halide-sensing protein YFP-H148Q/I152L. Screening 50,000 diverse, drug-like compounds...
Background and purpose: New antimicrobials are needed because of the emergence organisms that resistant to available antimicrobials. The purpose this study was evaluate a high‐throughput screening approach identify antibacterials against two common disease‐causing bacteria, determine frequency, novelty, potency compounds with antibacterial activity. Experimental approach: A high‐throughput, turbidometric assay bacterial growth in 96‐well plate format used screen diverse collection 150,000...
A new subspecies, Staphylococcus aureus subsp. anaerobius, is described on the basis of a study 84 strains isolated from young sheep affected by so-called "abscess disease.'' The this subspecies grow well under anaerobic conditions, but not at all or only very weakly aerobic conditions. They are catalase and benzidine negative form small unpigmented colonies. Anaerobically they produce l-lactic acid glucose. chemical composition cell wall, results deoxyribonucleic acid-deoxyribonucleic...
The study of Cosmic Rays (CRs) and Solar Energetic Particles (SEPs) is key in analyzing the effect solar activity on terrestrial environment. Changes properties medium they pass through until their detection profoundly affect intensity propagation direction CR flux.Our starting point that accurate measurements SEP flux can allow us to infer conditions way Earth, particularly interplanetary medium, magnetosphere atmosphere. development a simulation code helps perform such analysis, which may...
Aims: To determine the prevalence and characteristics of verotoxigenic Escherichia coli (VTEC), enteropathogenic E. (EPEC) necrotoxigenic (NTEC) in healthy cattle. Methods Results: Faecal samples from 412 cattle were screened for presence VTEC, EPEC NTEC. Four isolates each sample studied. NTEC isolated 8·7%, 8·2% 9·9% animals, respectively. VTEC more frequently calves heifers than adults. Seventy (4·2%), 69 (4·2%) 74 (4·5%) 1648 NTEC, Seventeen (24·3%) strains eae‐positive. Thirty‐six...
Functional studies in knockout mice indicate a critical role for urea transporters (UTs) the urinary concentrating mechanism and renal clearance. However, potent specific transport blockers have not been available. Here, we used high-throughput screening to discover high-affinity, small molecule inhibitors of UT-B transporter. A collection 50,000 diverse, drug-like compounds was screened using human erythrocyte lysis assay based on UT-B-facilitated acetamide transport. Primary yielded...
In Escherichia coli the genes involved in acquisition of tetracycline resistance are mainly tet(A) and tet(B). addition, tet(M) is most common determinant enterococci it associated with conjugative transposons plasmids. Although has been identified E. coli, to our knowledge, there no previous reports studying linkage gene different mobile genetic elements. The aim this study was determine occurrence tet(A), tet(B), doxycycline-resistant isolates from pigs, as well detection elements linked...
Two hundred and twenty-six attaching effacing Escherichia coli (AEEC) strains (20 enterohemorrhagic E. 206 atypical enteropathogenic coli) isolated from calves, lambs, goat kids with diarrhea healthy cattle, sheep, goats were tested for their resistance to 10 antimicrobial agents by the disc diffusion method. Resistant intermediate analyzed polymerase chain reaction presence of major genes. The overall percentage resistant tetracycline, streptomycin, erythromycin, sulfamethoxazole was very...
Attaching and effacing Escherichia coli (AEEC) strains isolated from diarrhoeic lambs goat kids were characterized for intimin (eae) EspB (espB) gene subtypes by PCR sequencing, genetic relatedness PFGE. Fifty (23 ovine 27 caprine) AEEC of 398 (246 152 analysed detected colony blot hybridization. These epidemiologically unrelated since they different outbreaks neonatal diarrhoea over a long period. Ovine belonged to serogroups O2, O4, O26, O80, O91 or untypable, caprine O3, O153 O163. Two...
Summary The in vitro activities of 15 antimicrobial agents against 195 strains Escherichia coli isolated from dairy calves affected by neonatal diarrhoea were determined. Of these 137 produced one or more potential virulence factors (F5, F41, F17, cytotoxic necrotizing factor, verotoxin and the eae gene), but remaining 58 did not produce any factors. overall percentage resistant to streptomycin, tylosin tetracycline was very high (above 65 %). A level resistance (from 23 50 %) ampicillin,...
Degenerate oligonucleotide primers based on internal peptide sequences obtained by HPLC from purified Staphylococcus aureus catalase were used to locate the S. and subsp. anaerobius kat regions PCR. Southern hybridization analysis with a probe derived 1·1 kb PCR-amplified fragment showed that single copy of putative gene was present in chromosome. The nucleotide sequence katA revealed 1518 bp open reading frame for protein 505 amino acids predicted molecular mass 58347 Da, whereas katB is...
Faecal samples from 146 diarrhoeic lambs and goat kids, 511 healthy sheep goats were screened for the presence of Vero cytotoxin-producing Escherichia coli (VTEC). In goats, VTEC isolated in 24·4 16·2% animals, respectively. Moreover, detected 3·1 5·9% These data suggest that seems not to be associated with diarrhoea kids. Only four strains eae -positive. The absence gene most these could indicate are less virulent humans classical -positive enterohaemorrhagic E. types. However, almost half...
Summary A study was made of the staphylococcal population on skin and nasal mucosa in milk 133 healthy goats. Of a total 346 strains isolated characterised as belonging to genus Staphylococcus , 74 (21.4%) were coagulase‐positive (68 S. aureus 6 hyicus ), 272 (78.6%) coagulase‐negative. The novobiocin‐sensitive species haemolyticus (23.5%), warneri (16.5%), epidermidis (11.8%), chromogenes (8.5%), caprae (6.6%) (2.6%), novobiocin‐resistant xylosus sciuri (7.4%), saprophyticus (4.8%), cohnii...
Staphylococcus aureus produces a variety of virulence factors that allow it to cause wide range infections in humans and animals. In the latter, S. is leading intramammary infections. The contribution catalase (KatA), an enzyme implicated oxidative stress resistance, beta-toxin (Hlb), haemolysin, pathogenesis poorly characterized. To investigate role these enzymes as potential aureus, we examined intracellular survival DeltakatA, Deltahlb DeltakatA mutants murine macrophages (J774A.1) bovine...
ABSTRACT The in vitro activities of several cephalosporins and quinolones against 195 strains Escherichia coli isolated from dairy calves affected by neonatal diarrhea were determined. One hundred thirty-seven these produced one or more potential virulence factors (F5, F41, F17, cytotoxic necrotizing factor, verotoxin, the eae gene), but remaining 58 did not produce any factors. From 11 to 18% E. resistant cephalothin, nalidixic acid, enoxacin, enrofloxacin. However, cefuroxime, cefotaxime,...