A5034765579- Particle accelerators and beam dynamics
- Gyrotron and Vacuum Electronics Research
- Particle Accelerators and Free-Electron Lasers
- Magnetic confinement fusion research
- Superconducting Materials and Applications
- Ion channel regulation and function
- Pulsed Power Technology Applications
- Plasma Diagnostics and Applications
- Nuclear Physics and Applications
- Cystic Fibrosis Research Advances
- Ion Transport and Channel Regulation
- Asthma and respiratory diseases
- X-ray Diffraction in Crystallography
- Nuclear reactor physics and engineering
- Crystallization and Solubility Studies
- Neonatal Respiratory Health Research
- Hearing, Cochlea, Tinnitus, Genetics
- Blood Coagulation and Thrombosis Mechanisms
- Ion Channels and Receptors
- Pancreatitis Pathology and Treatment
- Fusion materials and technologies
- Cardiac electrophysiology and arrhythmias
- Radiation Detection and Scintillator Technologies
- Ionosphere and magnetosphere dynamics
- Plasma Applications and Diagnostics
Yonsei University
2016-2025
Government of the Republic of Korea
2020
Pohang University of Science and Technology
2009-2018
San Francisco State University
2015
University of California, San Francisco
2008-2015
Incheon Medical Center
2014-2015
Pohang Accelerator Laboratory
1996-2012
Japan Atomic Energy Agency
2011
Princeton Plasma Physics Laboratory
1999-2011
Korea Institute of Fusion Energy
2011
Calcium-activated chloride channels (CaCCs) are widely expressed in mammalian tissues, including intestinal epithelia, where they facilitate fluid secretion. Potent, selective CaCC inhibitors have not been available. We established a high-throughput screen for identification of human based on inhibition ATP/carbachol-stimulated iodide influx HT-29 cells after lentiviral infection with the yellow fluorescent halide-sensing protein YFP-H148Q/I152L. Screening 50,000 diverse, drug-like compounds...
The Korea Superconducting Tokamak Advanced Research (KSTAR) project is the major effort of national fusion programme Republic Korea. Its aim to develop a steady state capable advanced superconducting tokamak establish scientific and technological basis for an attractive reactor. parameters are: radius 1.8 m, minor 0.5 toroidal field 3.5 T plasma current 2 MA, with strongly shaped cross-section double null divertor. initial pulse length provided by poloidal magnet system 20 s, but can be...
TMEM16A was found recently to be a calcium-activated CU channel (CaCC). CaCCs perform important functions in cell physiology, including regulation of epithelial secretion, cardiac and neuronal excitability, smooth muscle contraction. CaCC modulators are potential utility for treatment hypertension, diarrhea, cystic fibrosis. Screening drug natural product collections identified tannic acid as an inhibitor TMEM16A, with IC50 ∼ 6 µM ∼100% inhibition at higher concentrations. Tannic inhibited...
TMEM16A (ANO1) is a calcium-activated chloride channel (CaCC) expressed in secretory epithelia, smooth muscle, and other tissues. Cell-based functional screening of ~110,000 compounds revealed that activated CaCC conductance without increasing cytoplasmic Ca2+. By patch-clamp, N-aroylaminothiazole "activators" (Eact) strongly increased Cl- current at 0 Ca2+, whereas tetrazolylbenzamide "potentiators" (Fact) were not active Ca2+ but reduced the EC50 for Ca2+-dependent activation. Of 682...
Anoctamin1 (ANO1)/transmembrane protein 16A (TMEM16A), a calcium-activated chloride channel (CaCC), is involved in many physiological functions such as fluid secretion, smooth muscle contraction, nociception and cancer progression. To date, only few ANO1 inhibitors have been described, these low potency selectivity for ANO1. Here, we performed high-throughput screening to identify highly potent selective small molecule of Three novel were discovered from 54,400 synthetic molecules, they...
The extensive design effort for KSTAR has been focused on two major aspects of the project mission - steady-state-operation capability and advanced tokamak physics. steady state aspect is reflected in choice superconducting magnets, provision actively cooled in-vessel components, long pulse current drive heating systems. incorporated features associated with flexible plasma shaping, double null divertor passive stabilizers, internal control coils a comprehensive set diagnostics. Substantial...
Crofelemer, a purified proanthocyanidin oligomer extracted from the bark latex of <i>Croton lechleri</i>, is in clinical trials for secretory diarrheas various etiologies. We investigated antisecretory mechanism crofelemer by determining its effect on major apical membrane transport and signaling processes involved intestinal fluid transport. Using cell lines measurement procedures to isolate effects individual proteins, at 50 μM had little or no activity epithelial Na<sup>+</sup>...
A 20 MW/5 GHz lower hybrid current drive (LHCD) system was initially due to be commissioned and used for the second mission of ITER, i.e. Q = 5 steady state target. Though not part currently planned procurement phase, it is now under consideration an earlier delivery. In this paper, both physics technology conceptual designs are reviewed. Furthermore, appropriate work plan also developed. This design, R&D, installation a MW LHCD on ITER follows Scientific Technical Advisory Committee...
Mutations in the SLC26A4 gene are responsible for Pendred syndrome and non-syndromic hearing loss (DFNB4). This study analysed non-synonymous mutations newly identified East Asians, as well three common Caucasians, to characterise their molecular pathogenic mechanisms explore possibility of rescuing processing defects.A total 11 disease associated were generated effects on protein ion transporting activities examined.Most caused retention product (pendrin) intracellular region, while...
Azobenzene-based chloride transporters exhibit photoresponsive transport activities across lipid and plasma membranes.
<h3>Background</h3> Rotavirus is the most common cause of severe secretory diarrhoea in infants and young children globally. The rotaviral enterotoxin, NSP4, has been proposed to stimulate calcium-activated chloride channels (CaCC) on apical plasma membrane intestinal epithelial cells. We previously identified red wine small molecule CaCC inhibitors. <h3>Objective</h3> To investigate efficacy a extract synthetic molecule, CaCC<sub>inh</sub>-A01, inhibiting CaCCs diarrhoea. <h3>Design</h3>...
Anoctamin 1 (ANO1), a calcium-activated chloride channel, is highly amplified in prostate cancer, the most common form of cancer and leading causes death men, downregulation ANO1 expression or its functional activity known to inhibit cell proliferation, migration invasion cells. Here, we performed cell-based screening for identification inhibitors as potential anticancer therapeutic agents cancer. Screening ~300 selected bioactive natural products revealed that luteolin novel potent...
The expression levels of anoctamin 1 (ANO1, TMEM16A), a calcium-activated chloride channel (CaCC), are significantly increased in several tumors, and inhibition ANO1 is known to reduce cell proliferation migration. Here, we performed cell-based screening collection natural products drug-like compounds identify inhibitors ANO1. As result the screening, idebenone, miconazole plumbagin were identified as novel inhibitors. Electrophysiological studies showed that synthetic analog coenzyme Q10,...
Anoctamin1 (ANO1), a calcium-activated chloride channel, is involved in the proliferation, migration, and invasion of various cancer cells including head neck squamous cell carcinoma, lung cancer, prostate cancer. Inhibition ANO1 activity or downregulation expression these known to exhibit anticancer effects. Resveratrol, natural polyphenol abundant wines, grapes, berries, soybeans, peanuts, shows wide variety biological effects anti-inflammatory, antioxidant, activities. In this study, we...
Aberrant membrane transport caused by mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene is associated with a wide spectrum of respiratory and digestive diseases as well fibrosis. Using scanning method, we found 11 polymorphisms CFTR Korean population. Individual variants at these sites were analyzed conventional DNA screening 117 control 75 patients having bronchiectasis or chronic pancreatitis. In haplotype determination based on Bayesian algorithm, 15...
Disorganized ion transport caused by hypo- or hyperfunctioning of the cystic fibrosis transmembrane conductance regulator (CFTR) can be detrimental and may result in life-threatening diseases such as secretory diarrhea. Thus, CFTR is controlled elaborate positive negative regulations for an efficient homeostasis. It has been shown that expression activity regulated either positively negatively PDZ (PSD-95/discs large/ZO-1) domain-based adaptors. Although a regulation adaptors EBP50/NHERF1...
Inhibitors of the cystic fibrosis transmembrane conductance regulator (CFTR) chloride channel are predicted to slow cyst enlargement in polycystic kidney disease and reduce intestinal fluid loss secretory diarrheas. Screening ∼110000 small synthetic natural compounds for inhibition halide influx CFTR-expressing epithelial cells yielded a new class pyrimido-pyrrolo-quinoxalinedione (PPQ) CFTR inhibitors. Testing 347 analogues established structure−activity relationships. The most potent...
Chloride secretion by airway epithelial cells is defective in cystic fibrosis (CF). The conventional paradigm that CFTR activated through cAMP and protein kinase A (PKA), whereas the Ca(2+)-activated chloride channel (CaCC) Ca(2+) agonists like UTP. We found most current elicited primary cultures of human bronchial mediated a mechanism involving activation adenylyl cyclase I (AC1) cAMP/PKA signaling. Use selective inhibitors showed produced more from than CaCC. CFTR-dependent was reduced PKA...
Electron cyclotron resonance heating (ECRH)-assisted plasma breakdown is foreseen with full and half magnetic field in ITER. As reported earlier, the corresponding O1- X2-schemes have been successfully used to assist pre-ionization present-day devices. This contribution reports on common experiments studying effect of toroidal inclination ECR beam, which ⩾20° All devices could demonstrate successful assistance for this case also, although some necessary power was almost a factor 2 higher...