A5025714049- Brucella: diagnosis, epidemiology, treatment
- Burkholderia infections and melioidosis
- Salmonella and Campylobacter epidemiology
- Animal Diversity and Health Studies
- Monoclonal and Polyclonal Antibodies Research
- Microfluidic and Capillary Electrophoresis Applications
- Animal Disease Management and Epidemiology
- Escherichia coli research studies
- Microfluidic and Bio-sensing Technologies
- Acoustic Wave Resonator Technologies
- Bacteriophages and microbial interactions
- 3D Printing in Biomedical Research
- Innovative Microfluidic and Catalytic Techniques Innovation
- Prion Diseases and Protein Misfolding
- Galectins and Cancer Biology
Agricultural Research Center
2019-2025
Animal Health Research Institute
2023-2024
Egypt-Japan University of Science and Technology
2023
Ain Shams University
2023
Brucellosis is highly contagious bacterial zoonoses affecting a wide range of domesticated and wild animals. In this study, Brucella (B.) abortus bv 1 was identified in uterine discharge apparently healthy bitch queen with open pyometra housed on cattle farm. This study highlights the role dogs cats as symptomatic carriers reservoirs for Brucella. To best our knowledge, represents first report feline infection B. globally. These pet animals may contaminate environment infect both livestock...
Brucellosis is endemic in most parts of Egypt, where it caused mainly by Brucella melitensis biovar 3, and affects cattle small ruminants spite ongoing efforts devoted to its control. Knowledge the predominant species/strains circulating a region prerequisite brucellosis control strategy. For this reason study aiming at evaluation phenotypic genetic heterogeneity panel 17 spp. isolates recovered from domestic (cattle, buffalo, sheep, goat) four governorates during period five years...
Abstract Quartz crystal microbalance (QCM) is a versatile sensing platform that has gained increasing attention for its use in bioapplications due to high sensitivity, real-time measurement capabilities, and label-free detection. This article presents portable QCM system liquid biosensing uses modified Hartley oscillator drive 14 mm-diameter commercial sensors. The designed be low-cost, easy use, highly sensitive, making it ideal various bioapplications. A new flow cell design deliver...
Brucellosis is a highly contagious zoonosis worldwide with economic and public health impacts. The aim of the present study was to identify Brucella (B.) spp. isolated from animal populations located in different districts Egypt determine their antimicrobial resistance. In total, 34-suspected isolates were recovered lymph nodes, milk, fetal abomasal contents infected cattle, buffaloes, sheep, goats nine Egypt. identified by microbiological methods matrix-assisted laser desorption ionization...
Brucella melitensis is a serious public health threat, with human infection exhibiting acute febrile illness and chronic problems. The present study investigated the genetic diversity epidemiological links of important zoonotic bacterium B. in Egypt using multilocus variable-number tandem repeat analysis (MLVA-16) including eight minisatellite (panel 1) microsatellite 2, subdivided into 2A 2B) markers. A total 118 isolates were identified as biovar 3 by classical biotyping Bruce–ladder...
Brucellosis is a highly contagious zoonosis that occurs worldwide. Whole-genome sequencing (WGS) has become widely accepted molecular typing method for outbreak tracing and genomic epidemiology of brucellosis. Twenty-nine Brucella spp. (eight B. abortus biovar 1 21 melitensis 3) were isolated from lymph nodes, milk, fetal abomasal contents infected cattle, buffaloes, sheep, goats originating nine districts in Egypt. The isolates identified by microbiological methods matrix-assisted laser...
Brucellosis, caused by the bacteria of genus Brucella, is one most neglected common zoonotic diseases globally with a public health significance and high economic loss among livestock industry worldwide. Since little known about distribution B. abortus in Egypt, total 46 isolates recovered between 2012–2020, plus animal isolate from 2006, were analyzed examining whole core genome single nucleotide polymorphism (cgSNP) comparison to silico multilocus variable number tandem repeat analysis...
Trapezoidal cross-sectional spiral microfluidic channels showed high resolution and throughput in cell separation bio-applications. The main challenges are the complexity cost of fabrication process trapezoidal on micro-scale. In this work, we present application grayscale channel design to overcome process. We also use direct engraving with a CO2 laser beam polymethyl methacrylate (PMMA) material drastically reduce chip's (to <30 cents) time 20 min). capability methodology for sorting...
Brucellosis is one of the most important worldwide zoonoses many countries including Egypt. Camel brucellosis has not gained much attention in Egypt yet. This study focused on three governorates with highest camel populations and largest markets country to determine disease seroprevalence identify Brucella species local holdings. In total, 381 serum samples were collected from male female camels Giza, Aswan, Al-Bahr Al-Ahmar (the Red Sea) governorates. Samples serologically examined using...
Brucellae are Gram-negative, aerobic, non-motile coccobacilli causing brucellosis in man and animals. The disease is one of the most significant yet neglected global zoonoses. Especially developing countries, public health problems economic losses to private animal owners national revenues. Composed oligonucleotides, aptamers chemical analogues antibodies that promising components for aptamer-based rapid, sensitive, specific tests identify Brucella group bacteria. For this purpose, were...
The objective of the present study was to estimate seroprevalence Brucella spp. in humans and cattle at Sharkia Governorate, Egypt. In addition, identification milk samples by PCR culture with evaluation risk factors associated were carried out. Overall, antibodies examined 23.8%, while human participants it 21%. examination 205 using revealed that 6.3% positive for B. abortus biovar 1 results confirmed methods. Multivariate logistic regression consumption unpasteurized dairy products,...
Bovine brucellosis is a global zoonosis of public health importance. It an endemic disease in many developing countries including Pakistan. This study aimed to estimate the seroprevalence and molecular detection bovine assess association potential risk factors with test results. A total 176 milk 402 serum samples were collected from cattle buffaloes three districts upper Punjab, Milk investigated using ring (MRT), while sera tested by Rose–Bengal plate agglutination (RBPT) indirect...
Brucellosis is one of the most common neglected zoonotic diseases globally, with a public health significance and high economic loss in livestock industry caused by bacteria genus Brucella. In this study, 136 Egyptian Brucella melitensis strains isolated from animals humans between 2001 2020 were analysed examining whole-core-genome single-nucleotide polymorphism (cgSNP) comparison to silico multilocus variable number tandem repeat analysis (MLVA-16). Almost all isolates belonging West...
Abstract Quartz crystal microbalance (QCM) is a powerful sensing technique widely used in various applications, including biosensing, chemical analysis, and material science. In in-liquid QCM measurements are susceptible to fluctuations fluid flow rate, which can introduce unwanted noise compromise the accuracy reliability of measurements. this work, we present an approach enhance stability by utilizing microfluidic stabilizer. The stabilizer designed minimize rate fluctuations, thereby...
Brucellosis is a zoonotic disease affecting various animals, including camels. Our study aimed to isolate, identify, genotype the Brucella species circulating in camels and test antibiotic susceptibility of isolates against 8 antibacterial agents commonly prescribed for human brucellosis. Lymph nodes (n = 350) were collected from different Egyptian governorates. As identified by bacteriological Abortus meltensis ovis suis PCR, 14 recovered 350 lymph as B. melitensis biovar 3 (4%). The...
V eterinary healthcare encompasses various activities such as predic- tion, prevention, monitoring, diagnosis, and treatment of livestock.This article reviews the advantages challenges digitalizing animal health highlights its urgency in terms national security.The advent novel technologies their widespread use has facilitated tracking product movements supply chain, monitoring real-time, utilizing data analytics machine learning to identify patterns trends health.Technology made possible...