A5050432464- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Reproductive Physiology in Livestock
- Veterinary Equine Medical Research
- Birth, Development, and Health
- Seed Germination and Physiology
- Bee Products Chemical Analysis
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Renal and related cancers
- Medical and Biological Ozone Research
- Antibiotics Pharmacokinetics and Efficacy
- Antibiotic Resistance in Bacteria
- Gastrointestinal disorders and treatments
- Veterinary Oncology Research
- Genital Health and Disease
- Nail Diseases and Treatments
- SARS-CoV-2 detection and testing
- Milk Quality and Mastitis in Dairy Cows
- Reproductive System and Pregnancy
- Pharmacological Effects and Assays
- SARS-CoV-2 and COVID-19 Research
- Animal health and immunology
- Cervical Cancer and HPV Research
- Reproductive biology and impacts on aquatic species
- Travel-related health issues
Universidad Complutense de Madrid
2007-2023
The disease produced by the severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) is currently one of primary concerns worldwide. Knowing zoonotic origin and that several animal species, including dogs cats, are susceptible to viral infection, it critical assess relevance pets in this pandemic. Here, we performed a large-scale study on SARS-CoV-2 serological prevalence cats Spain order elucidate their role susceptibility. Samples from animals contact with COVID-19 positive...
Assisted reproductive technologies (ARTs) in the horse still yield suboptimal results terms of pregnancy rates. One reasons for this is lack optimal conditions sperm capacitation vitro. This study assesses use synthetic human tubal fluid (HTF) supplemented with D-penicillamine (HTF + PEN) vitro frozen/thawed stallion spermatozoa by examining capacitation-related events over 180 min incubation. Besides these events, we explored capacity to migrate thermotaxis and give rise a population...
The Purebred Spanish Horse, according to our clinical experience, is characterized by having a high number of stallions that do not meet the international commercial recommendations for equine-sperm cryopreservation. This means artificial insemination with frozen semen from these less widespread than in other breeds. In this study, we investigated if incorporation single-layer colloidal centrifugation prior cryopreservation conditions could increase ejaculates suitable processing, observing...
Contents Stallion spermatozoa recovered and examined immediately after colloidal centrifugation resulted in a higher straight‐line velocity (VSL) than sperm processed using direct conventional (p = 0.000), but there was no differences the progressive motility or DNA fragmentation (SDF) as determined by chromatin dispersion assay. However, when centrifuged were incubated at 37°C for 24 h to determine rate of SDF (r‐SDF), lower r‐SDF 0.0011) observed those separation (0.5 ± 0.1%/h) compared...
Six donkeys each received 2 mg/kg marbofloxacin as a 10 per cent aqueous solution administered intravenously. Principal pharmacokinetic parameters were determined and two efficacy indices computed by using selected mic90 values of against pathogenic equine strains to predict the drug at this dose. The pharmacokinetics in was characterised large mean volume distribution steady state (1.15 [0.09] l/kg) long (sd) elimination half-life 9.24 (1.96) hours. It also relatively slow total body...
Contents Sperm DNA fragmentation (sDF) is an important parameter to assessing sperm quality. Information about quality not available for donkeys, especially in some breeds at risk of extinction. The objectives this research were test the four commercial variants chromatin dispersion (SCD; Halomax test), originally developed assess sDF boars, bulls, rams and stallions, order scrutinize their applicability study a donkey breed extinction (Zamorano‐Leonesa), which there no specific analyze...
Abstract Background Equine embryos exhibit an unusual pattern of glucose tolerance in vitro and are currently cultured hyperglycaemic conditions. Objective Our main objective was to analyse the effect different concentrations on vitro‐produced equine embryo development quality. Study design Experiments comparing vivo produced embryos. Methods Oocytes (n = 641) were collected from post‐mortem ovaries, matured fertilised by intracytoplasmic sperm injection (ICSI). Embryo culture divided Day 0...
Variability among stallions in terms of semen cryopreservation quality renders it difficult to arrive at a standardized method. Different extenders and processing techniques (such us colloidal centrifugation) are used order optimize post-thaw sperm quality. Sperm chromatin integrity analysis is an effective tool for assessing such The aim the present study was compare effect two single layer centrifugation protocols (prior cryopreservation) combination with three commercial freezing on...
The study assessed the impact of four equine semen processing techniques on sperm quality and microbial load immediately post-processing after 48 h refrigeration. aim was to explore potential reduction prophylactic antibiotic usage in extenders. Semen from ten adult stallions collected processed under a strict hygiene protocol divided into aliquots: Simple Centrifugation with antibiotics (SC+), (SC−), Single-Layer Colloidal (CC−), Filtration (with SpermFilter®) (F−), all extenders without...
Abstract Kenney–Doig scale is considered the international standard method for classifying uterine biopsies in mares; however, its objectivity has been questioned by various studies. In present study, we analysed degree of agreement between two pathologists when assessing same set 201 biopsies, obtaining a slight to moderate level (κ = .34/κ w .57). Subsequently, developed numerical based on evaluation histological parameters, including inflammation, fibrosis, glandular density and lymphatic...
Current equine reproductive clinic aims to improve subfertile stallions semen quality, (which is furthermore affected by the processing protocol) and maintain this quality during storage. The aim of paper present a case referred Hospital Clínico Veterinario Complutense, stallion with low sperm 0% fertility rate in first batch mares served. Due these results it was decided perform special protocol, developed our laboratory, order increase its throughout colloidal centrifugation technique...