A5035438390- Microbial Natural Products and Biosynthesis
- Genomics and Phylogenetic Studies
- Microbial Community Ecology and Physiology
- Microbial Metabolism and Applications
- Fungal Biology and Applications
- Enzyme Production and Characterization
- Microbial Metabolic Engineering and Bioproduction
- Antibiotic Resistance in Bacteria
- Plant Disease Resistance and Genetics
- Antimicrobial Resistance in Staphylococcus
- Enzyme-mediated dye degradation
- Enzyme Catalysis and Immobilization
- Plant-Microbe Interactions and Immunity
- Bacteriophages and microbial interactions
- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Cancer therapeutics and mechanisms
- Mycobacterium research and diagnosis
- Biofuel production and bioconversion
- Synthetic Organic Chemistry Methods
- Carbohydrate Chemistry and Synthesis
- Bioactive Compounds and Antitumor Agents
- Molecular Biology Techniques and Applications
- Bacterial Identification and Susceptibility Testing
- Polymer Nanocomposites and Properties
University of Strathclyde
2013-2024
University of Ljubljana
2023
Texas Instruments (United States)
2012
Texas Instruments (United Kingdom)
2009-2010
University of Glasgow
1991-2007
Glasgow Royal Infirmary
2003-2007
Southern Cross University
2006
Massachusetts Institute of Technology
2003-2005
Bioscience (China)
2001
The Robertson Trust
1995
The hospital environment is particularly susceptible to contamination by bacterial pathogens that grow on surfaces in biofilms. effects of biocides two nosocomial pathogens, meticillin-resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa, growing as free-floating (planktonic) adherent biofilm populations (sessile) were examined. Clinical isolates MRSA P. aeruginosa grown biofilms discs materials found the (stainless steel, glass, polyethylene Teflon) treated with three commonly...
ObjectivesThe MBCs of three commonly used hospital biocides [containing quaternary ammonium compounds (QACs), chlorhexidine gluconate and triclosan] were determined for clinical isolates Staphylococcus aureus, which also screened genes encoding Qac efflux pumps.
The genome sequence of Mycobacterium tuberculosis has revealed the presence 20 different cytochrome P450 mono-oxygenases (P450s) within this organism, and subsequent sequences other mycobacteria Streptomyces coelicolor have indicated that these actinomycetes also large complements P450s, pointing to important physiological roles for enzymes. actinomycete P450s include homologues 14α-sterol demethylases, targets azole class drugs in yeast fungi. Previously, type was considered be absent from...
ABSTRACT Forty-seven strains representing 14 different Bacillus species isolated from clinical and food samples were grown in reconstituted infant milk formulae (IMF) subsequently assessed for adherence to, invasion of, cytotoxicity toward HEp-2 Caco-2 cells. Cell-free supernatant fluids 38 (81%) shown to be cytotoxic, 43 (91%) adhered the test cell lines, 23 (49%) demonstrated various levels of invasion. Of 21 cereus examined, 5 (24%) invasive. A larger percentage clinically derived (20%)...
The synthesis and properties of 80 short minor groove binders related to distamycin the thiazotropsins are described. design compounds was principally predicated upon increased affinity arising from hydrophobic interactions between DNA. introduction aromatic head groups, including quinolyl benzoyl derivatives, alkenes as linkers led several strongly active antibacterial with MIC for Staphylococcus aureus, both methicillin-sensitive -resistant strains, in range 0.1−5 μg mL-1, which is...
Antimicrobial resistance and the shortage of novel antibiotics have led to an urgent need for new antibacterial drug leads. Several existing natural product scaffolds (including chelocardins) not been developed because their suboptimal pharmacological properties could be addressed at time. It is demonstrated here that reviving such compounds through application biosynthetic engineering can deliver candidates. Through a rational approach, carboxamido moiety tetracyclines (an important...
ABSTRACT With the exceptions of Bacillus cereus and anthracis , species are generally perceived to be inconsequential. However, relevance other as food poisoning organisms etiological agents in nongastrointestinal infections is being increasingly recognized. Eleven isolated from veterinary samples associated with severe were assessed for presence expression diarrheagenic enterotoxins potential virulence factors. PCR studies revealed DNA sequences encoding hemolysin BL (HBL) enterotoxin...
The expansion of the genetic repertoire an organism by gene duplication or horizontal transfer (HGT) can aid adaptation.
ABSTRACT Most biosynthetic gene clusters (BGC) encoding the synthesis of important microbial secondary metabolites, such as antibiotics, are either silent or poorly expressed; therefore, to ensure a strong pipeline novel there is need develop rapid and efficient strain development approaches. This study uses comparative genome analysis instruct rational improvement, using Streptomyces rimosus , producer antibiotic oxytetracycline (OTC) model system. Sequencing genomes two industrial strains...
ABSTRACT We report the draft genome of Streptomyces rimosus (ATCC 10970), a soil isolate that produces oxytetracycline, commercially important and clinically useful antibiotic.
MGB-BP-3 is a potential first-in-class antibiotic, Strathclyde Minor Groove Binder (S-MGB), that has successfully completed Phase IIa clinical trials for the treatment of Clostridioides difficile associated disease. Its precise mechanism action and origin limited activity against Gram-negative pathogens are relatively unknown. Herein, with alone significantly inhibited bacterial growth Gram-positive, but not Gram-negative, bacteria as expected. Synergy assays revealed inefficient...
Infection control is a key aspect of wound management strategies. results in chemical imbalances and inflammation the may lead to prolonged healing times degradation surface. Frequent changing dressings result damage tissues an increased risk infection. This paper presents first from monitoring system that being developed detect presence growth bacteria real time. It based on impedance sensors could be placed at wound-dressing interface potentially monitor bacterial As wounds can produce...
Tetracyclines (TCs) are medically important antibiotics from the polyketide family of natural products. Chelocardin (CHD), produced by Amycolatopsis sulphurea, is a broad-spectrum tetracyclic antibiotic with potent bacteriolytic activity against number Gram-positive and Gram-negative multi-resistant pathogens. CHD has an unknown mode action that different TCs. It some structural features define it as 'atypical' and, notably, active tetracycline-resistant Identification characterization...
Oxytetracycline is a polyketide antibiotic made by Streptomyces rimosus. From DNA sequencing, the gene product of otcD1 deduced to function as bifunctional cyclase/aromatase involved in ring closure backbone. Although contiguous with ketoreductase gene, they are located an unusually large distance from genes encoding “minimal synthase” oxytetracycline cluster. A recombinant, disrupted genomic copy ofotcD1, four novel polyketides, all shorter chain length (by up 10 carbons) than...
ABSTRACT Physiological studies have shown that Streptomyces rimosus produces the polyketide antibiotic oxytetracycline abundantly when its mycelial growth is limited by phosphate starvation. We show here transcripts originating from promoter for one of biosynthetic genes, otcC (encoding anhydrotetracycline oxygenase), and a divergent otcX genes peak in abundance at onset production induced starvation, indicating synthesis controlled, least part, level transcription. Furthermore, analysis...
Oxytetracycline (OTC) is a 19-carbon polyketide antibiotic made by Streptomyces rimosus. The otcC gene encodes an anhydrotetracycline oxygenase that catalyzes hydroxylation of the anthracycline structure at position C-6 after biosynthesis backbone completed. A recombinant strain S. rimosus was disrupted in genomic copy synthesized novel C-17 polyketide. This result indicates absence product significantly influences ability OTC “minimal” synthase to make correct chain length. mutant...
Abstract BACKGROUND: The nature of the dispersion clay platelets in a resin composite will play an important role process enhancement physical properties that material. This paper examines how different modifiers and quantity surface treatment for Cloisite ® range organically modified clays affect situ polymerised poly(methyl methacrylate). Another which is mixture rod‐ platelet‐like minerals also investigated to understand shape particles can polymer properties. RESULTS: Five clays,...
Summary The fluid mosaic model has recently been amended to account for the existence of membrane domains enriched in certain phospholipids. In rod‐shaped bacteria, anionic phospholipid cardiolipin is at cell poles but its role morphogenesis filamentous bacterium Streptomyces coelicolor unknown. It was impossible delete clsA (cardiolipin synthase; SCO1389 ) unless complemented by a second copy elsewhere chromosome. When placed under control an inducible promoter, expression, profile and...