A5026352667- Genomics and Phylogenetic Studies
- Microbial Natural Products and Biosynthesis
- Bacteriophages and microbial interactions
- Plant Disease Resistance and Genetics
- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Neuroscience and Neuropharmacology Research
- Microbial Metabolism and Applications
- Neural dynamics and brain function
- Microbial Community Ecology and Physiology
- Probiotics and Fermented Foods
- Actinomycetales infections and treatment
- Mycobacterium research and diagnosis
- Plant-Microbe Interactions and Immunity
- Neuroscience and Neural Engineering
- Enterobacteriaceae and Cronobacter Research
- Legume Nitrogen Fixing Symbiosis
- Advanced Proteomics Techniques and Applications
- Enzyme Production and Characterization
- Molecular Biology Techniques and Applications
- Plant and fungal interactions
- Marine Sponges and Natural Products
- Metabolomics and Mass Spectrometry Studies
- Chemical Synthesis and Analysis
- Ion channel regulation and function
University of Strathclyde
2015-2024
University of Ljubljana
2023
Glasgow Life
2014
Swansea University
1995-2004
University of Wales
2002-2004
University of Tennessee Health Science Center
1991-2000
Le Bonheur Children's Hospital
1999
Children's Medical Center
1999
Wageningen University & Research
1994-1998
GTx (United States)
1997
1. We examined whether the three physiologically defined neuron types described for rodent neocortex were also evident in human association cortex studied an vitro brain slice preparation. relationship between physiological and morphological cell type neocortical neurons. In particular, we tested burst-firing neurons numerous regions of that are susceptible to seizures. 2. Although sampled regular-spiking fast-spiking neurons, observed no true as cortex. did find displayed a...
A new method for the isolation and enumeration of streptomycete spores from soil was developed. This makes use a cation-exchange resin to disperse particles. It allowed detection 10 in 100 g sterile soil, while ca. 3 could be accurately enumerated g. applied studying fate marked actinophage soil. In amended nonsterile relatively high numbers actinophages were only found during first few days experiment when host mycelial form. Later, after sporulation, lysogens detected still 60 inoculation....
Antimicrobial resistance and the shortage of novel antibiotics have led to an urgent need for new antibacterial drug leads. Several existing natural product scaffolds (including chelocardins) not been developed because their suboptimal pharmacological properties could be addressed at time. It is demonstrated here that reviving such compounds through application biosynthetic engineering can deliver candidates. Through a rational approach, carboxamido moiety tetracyclines (an important...
The model organism Streptomyces coelicolor represents a genus that produces vast range of bioactive secondary metabolites. We describe versatile procedure for systematic and comprehensive mutagenesis the S. genome. high-throughput process relies on in vitro transposon an ordered cosmid library; mutagenized cosmids with fully characterized insertions are then transferred by intergeneric conjugation into , where gene replacement is selected. can yield upward 90% genes, its application to...
We report the draft genome sequence of human pathogen Streptomyces somaliensis (DSM 40738), a within genus largely saprophytic organisms. S. causes severe and debilitating deep tissue bone infections. The is deposited in DDBJ/EMBL/GenBank with accession number AJJM01000000.
ABSTRACT The genome sequences of eight Streptomyces phages are presented, four which were isolated for this study. Phages R4, TG1, ϕHau3, and SV1 previously have been exploited as tools understanding genetically manipulating spp. We also extracted five apparently intact prophages from recent projects and, together with six phage genomes in the database, we analyzed all 19 a view to their relationships each other actinophages, particularly mycobacteriophages. Fifteen group into clusters...
Exposure to narrowband violet-blue light around 405 nm wavelength can induce lethal oxidative damage bacteria and fungi, however effects on viruses are unknown. As photosensitive porphyrin molecules involved in the microbicidal inactivation mechanism, since porphyrins absent viruses, then any damaging of might appear unlikely. This study used bacteriophage ɸC31, as a surrogate for non-enveloped double-stranded DNA establish whether virucidal effects. ɸC31 suspended minimal media,...
The coordination of chromosome segregation with cell growth is fundamental to the proliferation any organism. In most unicellular bacteria, strictly coordinated division and involves ParA that moves ParB nucleoprotein complexes bi- or unidirectionally toward pole(s). However, organization in multiploid, apically extending branching Streptomyces hyphae challenges known mechanisms bacterial segregation. complex life cycle two stages: vegetative sporulation. latter stage, multiple divisions...
ABSTRACT Most biosynthetic gene clusters (BGC) encoding the synthesis of important microbial secondary metabolites, such as antibiotics, are either silent or poorly expressed; therefore, to ensure a strong pipeline novel there is need develop rapid and efficient strain development approaches. This study uses comparative genome analysis instruct rational improvement, using Streptomyces rimosus , producer antibiotic oxytetracycline (OTC) model system. Sequencing genomes two industrial strains...
Abstract We investigated (1) the topography of projection neurons in nucleus basalis Meynert (NBM) with efferents to restricted regions primary somatosensory (SI), second (SII), and motor (MI) cortices rat; (2) percentage these NBM that were cholinergic; (3) collateralization, if any, single different subdivisions within SI, homotopic areas SI SII, MI. Retrograde single‐and double‐labeling techniques used study projections electrophysiologically identified representational Choline...
ABSTRACT We report the draft genome of Streptomyces rimosus (ATCC 10970), a soil isolate that produces oxytetracycline, commercially important and clinically useful antibiotic.
Comprehensive mutant libraries can be readily constructed by transposon mutagenesis. To systematically mutagenise the genome of Gram-positive bacterium Streptomyces coelicolor A3(2), we have employed high-throughput shuttle mutagenesis a cosmid library prepared in Escherichia coli. The location insertions is determined using automated procedures for isolation and DNA sequencing. However, major bottleneck was subsequent analysis sequence files. overcome this limitation, software application,...
Streptomyces coelicolor is a model actinomycete that well known for the diversity of its secondary metabolism and complex life cycle. As soil inhabitant, it exposed to heterogeneous frequently changing environmental circumstances. In present work, we studied effect diverse growth conditions phosphate depletion on lipid profile relationship between membrane composition development in S. coelicolor. The from cultures grown solid media, which closer natural habitat this microorganism, does not...
Tetracyclines (TCs) are medically important antibiotics from the polyketide family of natural products. Chelocardin (CHD), produced by Amycolatopsis sulphurea, is a broad-spectrum tetracyclic antibiotic with potent bacteriolytic activity against number Gram-positive and Gram-negative multi-resistant pathogens. CHD has an unknown mode action that different TCs. It some structural features define it as 'atypical' and, notably, active tetracycline-resistant Identification characterization...
Bacteria from the genus Streptomyces are among most complex of all prokaryotes; not only do they grow as a mycelium, also differentiate to form aerial hyphae before developing further spore chains. This developmental heterogeneity streptomycete microcolonies makes studying dynamic processes that contribute growth and development challenging procedure. As result, in order study mechanisms underpin growth, we have developed system for hyphal extension, protein trafficking, sporulation by...
Abstract The pattern of projections peripheral receptors to the neocortex in second somesthetic receiving area (SII) was mapped raccoons. purpose determine if projection forepaw SII region is disproportionally enlarged as it SI. Tungsten microelectrode recording procedures were used map thoroughly inferior wall suprasylvian sulcus for regions responsive mechanical stimulation receptors. results show that: shows an enlargement commensurate with that found This suggests those factors are...
We observed movies of replisome trafficking during Streptomyces coelicolor growth. A replisome(s) in the spore served as a replication center(s) until hyphae reached certain length, when tip-proximal formed and moved at fixed distance behind tip speed equivalent to extension rate tip.
The ventroposterior region of the thalamus mongrel cats was searched to locate zones activated by somatic stimuli. By using stimuli that selectively excited Pacinian corpuscles, areas this class afferent fibers were differentiated from regions other classes cutaneous mechanoreceptors. results showed inputs excite neurons in inferior nucleus (VPI) thalamus, whereas more dorsal within receive mechanoreceptor classes. This definition VPI tended be larger and extend further lateral than some...