A5070282767- Plant nutrient uptake and metabolism
- Plant Micronutrient Interactions and Effects
- Aluminum toxicity and tolerance in plants and animals
- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Cellular transport and secretion
- Ion channel regulation and function
- Myasthenia Gravis and Thymoma
- Legume Nitrogen Fixing Symbiosis
- Plant Stress Responses and Tolerance
- Arsenic contamination and mitigation
- Plant Reproductive Biology
- Single-cell and spatial transcriptomics
- Plant-Microbe Interactions and Immunity
- Lipid metabolism and biosynthesis
- DNA Repair Mechanisms
- Genetic factors in colorectal cancer
- Plant Pathogenic Bacteria Studies
- Advanced Fluorescence Microscopy Techniques
- Nicotinic Acetylcholine Receptors Study
- Cancer Genomics and Diagnostics
- ATP Synthase and ATPases Research
- Plant tissue culture and regeneration
- Polysaccharides and Plant Cell Walls
- Phosphorus and nutrient management
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2003-2024
CEA Cadarache
2001-2024
Institut de Biosciences et Biotechnologies
2001-2024
Centre National de la Recherche Scientifique
2004-2024
Aix-Marseille Université
2018-2023
Institute of Environmental Biology and Biotechnology
2022
Laboratoire de Biologie du Développement
2001-2021
University of Southern California
1997
Inserm
1992-1993
Institut de Neurobiologie de la Méditerranée
1992
AtHMA4 is an Arabidopsis thaliana P 1B ‐ATPase which transports Zn and Cd. Here, we demonstrate that localized at the plasma membrane expressed in tissues surrounding root vascular vessels. The ectopic overexpression of improved growth presence toxic concentrations Zn, Cd Co. A null mutant exhibited a lower translocation from roots to shoot. In contrast, overexpressing lines displayed increase zinc cadmium shoot content. Altogether, these results strongly indicate plays role metal loading xylem.
Compartmentation of the eukaryotic cell requires a complex set subcellular messages, including multiple retrograde signals from chloroplast and mitochondria to nucleus, regulate gene expression. Here, we propose that one such signal is phosphonucleotide (3'-phosphoadenosine 5'-phosphate [PAP]), which accumulates in Arabidopsis thaliana response drought high light (HL) stress enzyme SAL1 regulates its levels by dephosphorylating PAP AMP. chloroplasts but not cytosol. sal1 mutants accumulate...
Abstract Environmental cues profoundly modulate cell proliferation and elongation to inform direct plant growth development. External phosphate (Pi) limitation inhibits primary root in many species. However, the underlying Pi sensory mechanisms are unknown. Here we genetically uncouple two sensing pathways apex of Arabidopsis thaliana . First, rapid inhibition transition zone is controlled by transcription factor STOP1, its target, ALMT1 , encoding a malate channel, ferroxidase LPR1, which...
Immunoglobulin G fractions from patients with Lambert-Eaton myasthenic syndrome (LEMS), an autoimmune disease of neuromuscular transmission, immunoprecipitate 125I-labeled omega-conotoxin GVIA-labeled calcium channels solubilized rat brain. A 58-kDa antigen was detected by probing Western blots partially purified LEMS plasma and IgG shown to be the relevant in receptor immunoprecipitation. Monoclonal antibody 1D12, produced immunizing mice synaptic membranes, has properties similar these...
Abstract Arabidopsis (Arabidopsis thaliana) absorbs inorganic phosphate (Pi) from the soil through an active transport process mediated by nine members of PHOSPHATE TRANSPORTER1 (PHT1) family. These proteins share a high level similarity (greater than 61%), with overlapping expression patterns. The resulting genetic and functional redundancy prevents analysis their specific roles. To overcome this difficulty, our approach combined several mutations gene silencing to inactivate multiple PHT1...
Summary Low‐phosphate (Pi) conditions are known to repress primary root growth of Arabidopsis at low pH and in an Fe‐dependent manner. This arrest requires accumulation the transcription factor STOP1 nucleus, where it activates malate transporter gene ALMT1 ; exuded is suspected interact with extracellular Fe inhibit growth. In addition, ALS3 – ABC‐like identified for its role tolerance toxic Al represses nuclear expression . Until now was unclear whether Pi deficiency itself or nucleus....
Summary RNA transcript levels of Arabidopsis plants, infected by the rhizobacterium Pseudomonas thivervalensis (strain MLG45), and axenic control plants were compared using cDNA microarrays representing approximately 14 300 genes. The analysis revealed an increase defence‐related transcripts in shoots bacterized relative to (axenic) plants. These modifications confirmed physiological experiments. Plants with P. more resistant subsequent infections virulent pathogen syringae pv. tomato...
Aluminum (Al) toxicity and inorganic phosphate (Pi) limitation are widespread chronic abiotic mutually enhancing stresses that profoundly affect crop yield. Both strongly inhibit root growth, resulting from a progressive exhaustion of the stem cell niche. Here, we report on casein kinase 2 (CK2) inhibitor identified by its capability to maintain functional niche in Arabidopsis thaliana under Al toxic conditions. CK2 operates through phosphorylation cycle checkpoint activator SUPPRESSOR OF...
Soil availability of inorganic ortho-phosphate (PO43−, Pi) is a key determinant plant growth and fitness.1Bieleski R.L. Phosphate pools, phosphate transport, availability.Annu. Rev. Plant Physiol. 1973; 24: 225-252Crossref Google Scholar Plants regulate the capacity their roots to take up by adapting abundance H+-coupled transporters PHOSPHATE TRANSPORTER 1 (PHT1) family2Nussaume L. Kanno S. Javot H. Marin E. Pochon N. Ayadi A. Nakanishi T.M. Thibaud M.C. import in plants: focus on PHT1...
Background Mutations in the FRY1/SAL1 Arabidopsis locus are highly pleiotropic, affecting drought tolerance, leaf shape and root growth. FRY1 encodes a nucleotide phosphatase that vitro has inositol polyphosphate 1-phosphatase 3′,(2′),5′-bisphosphate activities. It is not clear which activity mediates each of diverse biological functions planta. Principal Findings A fry1 mutant was identified genetic screen for mutants deregulated expression Pi High affinity Transporter 1;4 (PHT1;4)....
Inorganic phosphate (Pi) is the sole source of phosphorus for plants. It a limiting factor plant yield in most soils worldwide. Due to economic and environmental constraints, use Pi fertilizer will be more limited. Unfortunately, evaluation bioavailability or starvation traits remains tedious task, which often does not inform us about real status.Here, we identified by transcriptomic studies carried out model Arabidopsis thaliana, early roots- leaves-conserved molecular markers starvation,...
SUMMARY STOP1, an Arabidopsis transcription factor favouring root growth tolerance against Al toxicity, acts in the response to iron under low Pi (−Pi). Previous studies have shown that and Fe regulate stability accumulation of STOP1 roots, protein is sumoylated by unknown E3 ligase. Here, using a forward genetics suppressor screen, we identified SUMO (small ubiquitin‐like modifier) ligase SIZ1 as modulator signalling. Mutations increase expression ALMT1 (a direct target STOP1) responses...
Phosphate starvation-mediated induction of the HAD-type phosphatases PPsPase1 (AT1G73010) and PECP1 (AT1G17710) has been reported in Arabidopsis (Arabidopsis thaliana). However, little is known about their vivo function or impact on plant responses to nutrient deficiency. The preferences for different substrates have studied vitro but require confirmation planta. Here, we examined both enzymes using a reverse genetics approach. We demonstrated that affect phosphocholine phosphoethanolamine...
The presence of synaptic proteins involved in excitation/secretion coupling was examined ten small cell lung cancer lines. N‐Type calcium channels (ω)‐conotoxin receptors), synaptotagmin (p65) and syntaxin (HPC‐1) were detected eight. Co‐immunoprecipitation experiments indicated that can form a complex with channels. expression may elicit an autoimmune response reduces transmitter release at the nerve terminal.
Aluminum (Al) is a major limiting factor for crop production on acidic soils, inhibiting root growth and plant development. At pH (pH < 5.5), Al3+ ions are the main form of Al present in media. have an increased solubility at 5.5 result toxicity. higher pH, free fraction decreases media, but whether plants can detect these pHs remain unknown. To cope with stress, SENSITIVE TO PROTON RHIZOTOXICITY1 (STOP1) transcription induces AL-ACTIVATED MALATE TRANSPORTER1 (ALMT1), malate-exuding...
The regulation of intracellular pyrophosphate (PPi) level is crucial for proper morphogenesis across all taxonomic kingdoms. PPi released as a byproduct from ~200 metabolic reactions, then hydrolyzed by either membrane-bound (H+-PPase) or soluble pyrophosphatases (PPases). In Arabidopsis, the loss vacuolar H+-PPase/FUGU5, key enzyme in homeostasis, results delayed growth and number developmental defects, pointing to importance homeostasis plant morphogenesis. Arabidopsis genome encodes...
Abstract New regulatory functions in plant development and environmental stress responses have recently emerged for a number of apocarotenoids produced by enzymatic or nonenzymatic oxidation carotenoids. β-Cyclocitric acid (β-CCA) is one such compound derived from β-carotene, which triggers defense mechanisms leading to marked enhancement tolerance drought stress. We show here that this response associated with an inhibition root growth affecting both cell elongation division. Remarkably,...
DNA synthesis and postreplication mismatch repair were measured in vitro using cell-free extracts from cultured human SY5Y neuroblastoma WI38 fibroblast cells different growth states. All extracts, including differentiated quiescent fibroblasts, catalyzed SV40 origin-dependent synthesis, totally dependent on T-antigen. Thus, although fibroblasts essentially nondividing, their competent for replication polymerases δ, α, possibly ε, with proliferating cell nuclear antigen. Nonreplicative...