A5101461131- Plant Molecular Biology Research
- Plant tissue culture and regeneration
- Polyamine Metabolism and Applications
- Plant Genetic and Mutation Studies
- Plant Virus Research Studies
- Protein Kinase Regulation and GTPase Signaling
- Plant Reproductive Biology
- Renal and related cancers
- Caveolin-1 and cellular processes
- Genomic variations and chromosomal abnormalities
- Reproductive Biology and Fertility
- Single-cell and spatial transcriptomics
- Animal Genetics and Reproduction
- CRISPR and Genetic Engineering
- Cancer Research and Treatments
- Hand Gesture Recognition Systems
- Sleep and Work-Related Fatigue
- Sphingolipid Metabolism and Signaling
- Proteoglycans and glycosaminoglycans research
- Cancer-related molecular mechanisms research
- Botanical Research and Chemistry
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Date Palm Research Studies
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Cell death mechanisms and regulation
Hiroshima Institute of Technology
2018-2024
Tokyo University of Science
2023
National Institute for Basic Biology
2008-2020
Okayama University
2006-2019
Japan Science and Technology Agency
2011-2017
Osaka University
2007-2016
Duke University
2013
Hokkaido University
2002-2008
National Institute for Environmental Studies
2004
Gifu University
1994-2000
Abstract The cellular polyamines putrescine, spermidine, and spermine are ubiquitous in nature have been implicated a wide range of growth developmental processes. There is little information, however, on mutant plants or animals defective the synthesis polyamines. Arabidopsis genome has two genes encoding spermidine synthase, SPDS1 SPDS2. In this paper, we describe T-DNA insertion mutants both these genes. While each allele shows normal growth, spds1-1 spds2-1 double-mutant seeds abnormally...
Loss-of-function mutants of the Arabidopsis thaliana ACAULIS 5 (ACL5) gene, which encodes spermine synthase, exhibit a severe dwarf phenotype. To elucidate ACL5-mediated regulatory pathways stem internode elongation, we isolated four suppressor acaulis (sac) that reverse acl5 Because these do not rescue dwarfism known phytohormone-related mutants, SAC genes appear to act specifically on ACL5 pathways. We identify gene responsible for dominant sac51-d mutant, almost completely suppresses...
Spermine is the final product of polyamine biosynthetic pathway and ubiquitously present in most organisms. The genome Arabidopsis thaliana has two genes encoding spermine synthase: ACAULIS5 ( ACL5 ), whose loss‐of‐function mutants show a severe defect stem elongation, SPMS . In order to elucidate function plants, we isolated T‐DNA insertion mutant gene. Free conjugated levels mutant, designated spms‐1 , were significantly decreased compared with those wild‐type, but no obvious morphological...
During regeneration, differentiated plant cells can be reprogrammed to produce stem cells, a process that requires coordination of cell cycle reactivation with acquisition other cellular characteristics. However, the factors coordinate two functions during reprogramming have not been determined. Here, we report link between and new cell-type characteristics through activity cyclin-dependent kinase A (CDKA) in moss Physcomitrella patens. Excised gametophore leaf P. patens are readily...
Inducible transgene expression provides a useful tool to analyze gene function. The moss Physcomitrella patens is model basal land plant with well-developed research tools, including high efficiency of targeting and substantial genomics resources. However, current systems for controlled remain limited. Here we report the development an estrogen receptor mediated inducible system, based on system used in flowering plants. After identifying appropriate promoters drive chimeric transducer,...
The Arabidopsis genome contains four genes that encode proteins similar to both spermidine synthase and spermine of other organisms. Our previous study revealed one these genes, designated ACAULIS5 ( ACL5 ), encodes its null mutation results in a severe defect the elongation stem internodes. Here we report characterization three SPDS1 , SPDS2 SPDS3 . showed possess activity yeast synthase‐deficient mutants, but enzyme remained be determined. RNA gel blot analysis all are expressed plant...
Disruption of the Arabidopsis thaliana ACAULIS5 (ACL5) gene, which has recently been shown to encode thermospermine synthase, results in a severe dwarf phenotype. A previous study showed that sac51-d, dominant suppressor mutant acl5-1, premature termination codon an upstream open reading frame (ORF) SAC51, encodes putative transcription factor, and suggested involvement ORF-mediated translational control ACL5-dependent stem elongation. Here we report identification gene responsible for...
Abstract Both land plants and metazoa have the capacity to reprogram differentiated cells stem cells. Here we show that moss Physcomitrella patens Cold-Shock Domain Protein 1 (PpCSP1) regulates reprogramming of leaf chloronema apical shares conserved domains with induced pluripotent cell factor Lin28 in mammals. PpCSP1 accumulates is maintained throughout process resultant Expression negatively regulated by its 3′-untranslated region (3′-UTR). Removal 3′-UTR stabilizes transcripts, results...
Next-generation sequencing technologies have made it possible to carry out transcriptome analysis at the single-cell level. Single-cell RNA-sequencing (scRNA-seq) data provide insights into cellular dynamics, including intercellular heterogeneity as well inter- and intra-cellular fluctuations in gene expression that cannot be studied using populations of cells. The utilization scRNA-seq is, however, restricted cell types can isolated from their original tissues, difficult obtain precise...
Thermospermine acts in negative regulation of xylem differentiation and its deficient mutant Arabidopsis thaliana, acaulis5 (acl5), shows excessive formation severe dwarfism. Studies two dominant suppressors acl5, sac51-d sac52-d, have revealed that SAC51 SAC52 encode a transcription factor ribosomal protein L10 (RPL10), respectively, these mutations enhance translation the mRNA, which contains conserved upstream open reading frames 5’ leader. Here we report identification SAC53 SAC56...
Under certain circumstances differentiated cells can be reprogrammed to form stem in land plants, but only a portion of the reprograms successfully. A long-distance inhibitory signal from surrounding has been reported some ferns. Here we show existence anisotropic regulate cell formation moss Physcomitrella patens. When single were isolated gametophore leaf, over 90% them with characteristic nuclear expansion. By contrast, when two adjacent isolated, nuclei both expanded, successful...
Polyamines are small basic compounds present in all living organisms and act a variety of biological processes. However, the mechanism polyamine sensing, signaling response relation to other metabolic pathways remains be fully addressed plant cells. As one approach, we isolated Arabidopsis mutants that show increased resistance spermine terms chlorosis. We here two have point mutation nitrate transporter gene NRT1/PTR family (NPF), NRT1.3 (AtNPF6.4). These also exhibit putrescine spermidine...
ACAULIS5 (ACL5) encodes thermospermine synthase in Arabidopsis and its loss-of-function mutant acl5 shows excess xylem differentiation severe dwarfism. SAC51 a basic helix-loop-helix (bHLH) protein was identified from sac51-d, dominant suppressor of acl5, which restores the wild-type phenotype without thermospermine. The 5' leader mRNA contains multiple upstream open-reading frames (uORFs) sac51-d has premature stop codon fourth uORF. This uORF is conserved among family genes vascular...
Sphingomyelin phosphodiesterase 3 ( Smpd3), which encodes neutral sphingomyelinase 2 (nSMase2), is a key molecule for skeletal development as well the cytodifferentiation of odontoblasts and alveolar bone. However, effects nSMase2 on periodontal ligament (PDL) cells are still unclear. In this study, authors analyzed Smpd3 human PDL (HPDL) cells. The found that increases mRNA expression calcification-related genes, such alkaline phosphatase (ALPase), type I collagen, osteopontin, Osterix...
Regulation of phosphatidylinositol kinase (EC 2.7.1.67) and 4-phosphate (PtdIns4P) 2.7.1.68) was investigated in highly enriched plasma-membrane cytosolic fractions derived from cloned rat pituitary (GH3) cells. In plasma membranes, 4,5-bisphosphate [PtdIns(4,5)P2] added exogenously enhanced incorporation [32P]phosphate [gamma-32P]MgATP2- into PtdIns(4,5)P2 PtdIns4P to 150% control; half-maximal effect occurred with 0.03 mM exogenous PtdIns(4,5)P2. Exogenous (PtdIns) had no effect. When...
Polyamines (putrescine, spermidine and spermine) are ubiquitously present in various types of cells living organisms. They involved a variety cellular processes, including cell proliferation differentiation, required for abiotic stress tolerances plants. However, it is still not understood whether polyamines the plant growth inhibition caused by DNA-damaging agents. In this study, we examined effects on root gene expression Arabidopsis thaliana treated with mitomycin C (MMC), genotoxic agent...
Male and female reproductive tracts develop from anterior intermediate mesoderm with similar differentiation processes. The develops into the mesonephros, Wolffian duct initiates by epithelialization in mesonephros. Müllerian invaginates coelomic epithelium of cranial mesonephros for ductal formation is then regionalized proximal to caudal tracts. In this study, we focused on duct, initiation regionalization step ducts as a continuous process. By using mesodermal cells mouse pluripotent stem...