A5043980238- Genetic diversity and population structure
- Chromosomal and Genetic Variations
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Plant Genetic and Mutation Studies
- Identification and Quantification in Food
- Plant tissue culture and regeneration
- Genomics and Phylogenetic Studies
- GABA and Rice Research
- Fish Biology and Ecology Studies
- Amphibian and Reptile Biology
- Rice Cultivation and Yield Improvement
- Reproductive biology and impacts on aquatic species
- Plant Virus Research Studies
- Agricultural pest management studies
- Genetic Mapping and Diversity in Plants and Animals
- Plant Molecular Biology Research
- Ichthyology and Marine Biology
- Animal Behavior and Reproduction
- Soybean genetics and cultivation
- Plant and animal studies
- Wildlife Ecology and Conservation
- Plant Gene Expression Analysis
- Venomous Animal Envenomation and Studies
- Transgenic Plants and Applications
- Insect Resistance and Genetics
Kasetsart University
2014-2023
Naresuan University
2016-2020
Queen Saovabha Memorial Institute
2016
Thai Red Cross Society
2016
Zoological Park Organization
2013
Kyoto University
2013
Nagoya University
2013
Hokkaido University
1992-2013
National Institute for Basic Biology
1999
Tokyo University of Agriculture and Technology
1989
DNA barcodes of mitochondrial COI and Cytb genes were constructed from 54 specimens 16 species for identification. Intra- interspecific sequence divergence the gene (10 times) was greater than that (4 times), which suggests former may be a better marker latter delimitation in snakes. The barcode cut-off scores differed by more 3% between most species, minimum maximum intraspecific divergence. Clustering analysis indicated fell into monophyletic clades. These results suggest these could...
Unlike the chromosome constitution of most snakes (2n=36), cobra karyotype shows a diploid number 38 with highly heterochromatic W and large morphologically different 2. To investigate process sex differentiation evolution between cobras, snakes, other amniotes, we constructed map Siamese (Naja kaouthia) 43 bacterial artificial chromosomes (BACs) derived from chicken zebra finch libraries using fluorescence in situ hybridization (FISH) technique, compared it those chicken, finch, amniotes....
An investigation of sex-specific loci may provide important insights into fish sex determination strategies. This be useful for biotechnological purposes, example, to produce all-male or all-female commercial breeding. The North African catfish species, Clarias gariepinus , has been widely adopted aquaculture because its superior growth and disease resistance render the species suitable hybridization with other improve productivity quality meat. either a ZZ/ZW XX/XY system. Here, we...
The African catfish (Clarias gariepinus) may exhibit the co-existence of XX/XY and ZZ/ZW sex-determination systems (SDSs). However, SDS might be influenced by a polygenic (PSD) system, comprising multiple independently segregating sex "switch" loci to determine within species. Here, we aimed detect existence PSD using hybrid. hybrid produced crossing male with female bighead (C. macrocephalus, XX/XY) is good animal model study SDSs. Determining can help in understanding interactions between...
The Siamese crocodile (Crocodylus siamensis) and Saltwater (C. porosus) are two of the most endangered animals in Thailand. Their numbers have been reduced severely by hunting habitat fragmentation. A reintroduction plan involving captive-bred populations that used commercially is important necessary as a conservation strategy to aid recovery wild populations. Here, genetic diversity population structure 69 individual crocodiles, mostly members captive populations, were analyzed using both...
Squamate reptile chromosome 2 (SR2) is thought to be an important remnant of ancestral amniote super-sex chromosome, but a recent study showed that the Siamese cobra W sex also part this larger chromosome. To confirm existence and understand mechanisms evolution, maps two snake species (Russell's viper: Daboia russelii [DRU] common tiger snake: Notechis scutatus [NSC]) were constructed using bacterial artificial chromosomes (BACs) derived from chicken zebra finch libraries containing...
Two novel repetitive DNA sequences, VSAREP1 and VSAREP2, were isolated from the water monitor lizard (Varanus salvator macromaculatus, Platynota) characterized using molecular cytogenetics. The respective lengths guanine-cytosine (GC) contents of sequences 190bp 57.5% for 185bp 59.7% both elements tandemly arrayed as satellite in genome. VSAREP2 each located at C-positive heterochromatin pericentromeric region chromosome 2q, centromeric 5, 3 pairs microchromosomes. This suggests that genomic...
Satellite DNAs (stDNAs) are highly repeated sequences that constitute large portions of any genome. The evolutionary dynamics stDNA (e.g. copy number, nucleotide sequence, location) can, therefore, provide an insight into genome organization and evolution. We investigated the origin VSAREP in 17 monitor lizards (seven Asian, five Australian, African) at molecular cytogenetic level. Results revealed is conserved Asian Australian varanids, but not African suggesting these either differentiated...
Abstract To better understand PBI-DdeI satellite DNA located in the centromeric region of python, molecular evolution analysis was conducted on 40 snake species. A ladder-like pattern bands with repetition 194–210 bp monomer observed 15 species using PCR. Molecular cloning performed to obtain 97 AT-rich sequences. Phylogenetic and network analyses showed three subfamilies sequences grouped species-specific clusters, suggesting rapid evolution. Slow found eight shared sequences, recent...
Captive breeding programs for endangered species can increase population numbers eventual reintroduction to the wild. populations are typically small and isolated, which results in inbreeding reduction of genetic variability, may lead an increased risk extinction. The Omkoi Wildlife Breeding Center maintains only Thai captive Chinese goral (Naemorhedus griseus) population, has plans reintroduce individuals into natural isolated populations. Genetic variability was assessed within using...
Several parameters affecting transient β-glucuronidase (GUS) expression in protocorms of Dendrobium cv. Jaquelyn Thomas were examined using bombardment technique with pActin-1D plasmid. The GUS activity protocorm was not significantly affected by size the target, type particles, and helium gas pressure. However, numbers surviving tissues after different. Transgenic orchids established actively dividing gold particles coated pMNK1005 plasmid containing hygromycin phosphotransferase (hgh)...
Genomic alteration in head and neck squamous cell carcinoma (HNSCC) was studied two line pairs (HN30-HN31 HN4-HN12) using conventional C-banding, multiplex fluorescence situ hybridization (M-FISH), array comparative genomic (array CGH). HN30 HN4 were derived from primary lesions the pharynx base of tongue, respectively, HN31 HN12 lymph-node metastatic belonging to same patients. Gain chromosome 1, 7, 11 shared almost all lines. Hierarchical clustering revealed that closely related HN4, which...
Snakes exhibit genotypic sex determination with female heterogamety (ZZ males and ZW females), the state of chromosome differentiation also varies among lineages. To investigate evolutionary history homologous genes located in nonrecombining region differentiated chromosomes snakes, partial sequences gametologous CTNNB1 gene were analyzed for 12 species belonging to henophid (Cylindrophiidae, Xenopeltidae, Pythonidae) caenophid snakes (Viperidae, Elapidae, Colubridae)....
The genera Amphiprion and Premnas comprise the common anemonefish that are widely distributed in tropical areas. Species identification of these two is difficult due to high, intraspecific, morphological variation. Recently, DNA barcoding has been employed as an efficient tool uses a short genetic marker organism's enable recognition cryptic species. This study applied three regions mitochondrial DNA—cytochrome c oxidase I (COI), cytochrome b (Cytb) 16S rRNA—as barcodes for species seven one...
Abstract Sex identification provides important information for ecological and evolutionary studies, as well benefiting snake conservation management. Traditional methods such cloacal probing or popping are counterproductive sex concerning very small species, resulting in difficulties the management of their breeding programs. In this study, nucleotide sequences gametologous genes ( CTNNB 1 WAC genes) were used development molecular sexing markers caenophidian snakes. Two candidate developed...
Ten novel microsatellite markers were developed and characterized from Siamese fighting fish (Betta splendens). Nine of ten polymorphic, exhibiting an allelic number (NA) 2 to 6 alleles per locus. The effective (NE) ranged 1.60 3.08 (average 2.30). observed (HO) expected (HE) heterozygosities 0.13 0.67 0.39) 0.29 0.63 0.50), respectively. Linkage disequilibrium was not significantly detected for any pair loci, only two loci (BettaMS23 BettaMS28) showed significant deviations Hardy-Weinberg...
The fragmentation of habitats and hunting have impacted the Asian woolly-necked stork (Ciconia episcopus), leading to a serious risk extinction in Thailand. Programs active captive breeding, together with careful genetic monitoring, can play an important role facilitating creation source populations variability aid recovery endangered species. Here, diversity population structure 86 storks from three breeding programs [Khao Kheow Open Zoo (KKOZ) comprising 68 individuals, Nakhon Ratchasima...