A5004412252- Chromosomal and Genetic Variations
- Plant Molecular Biology Research
- RNA and protein synthesis mechanisms
- Plant nutrient uptake and metabolism
- Bacterial Genetics and Biotechnology
- Plant Reproductive Biology
- Genetic Mapping and Diversity in Plants and Animals
- CRISPR and Genetic Engineering
- Telomeres, Telomerase, and Senescence
- Bacteriophages and microbial interactions
- Genomics and Phylogenetic Studies
- Advanced biosensing and bioanalysis techniques
- DNA Repair Mechanisms
- Plant Gene Expression Analysis
- Genetic Syndromes and Imprinting
- RNA Research and Splicing
- Genomics and Chromatin Dynamics
- Plant Stress Responses and Tolerance
- Epigenetics and DNA Methylation
- DNA and Nucleic Acid Chemistry
- Antibiotic Resistance in Bacteria
- Plant Virus Research Studies
- RNA modifications and cancer
- Medical and Biological Sciences
- Cancer therapeutics and mechanisms
Pioneer Hi-Bred
2007-2019
Corteva (United States)
2018-2019
South Johnston High School
2011
DuPont (United States)
2010
Experimental Station
2006-2008
Bielefeld University
2003
Pioneer (Japan)
2003
Florida State University
2003
Massachusetts Institute of Technology
1993-1999
Institute of Molecular Genetics
1979-1992
The mobile floral-promoting signal, florigen, is thought to consist of, in part, the FT protein named after Arabidopsis thaliana gene FLOWERING LOCUS T. transcribed and translated leaves its moves via phloem shoot apical meristem where it promotes transition from vegetative reproductive development. In our search for a maize FT-like floral activator(s), seven Zea mays CENTRORADIALIS (ZCN) genes encoding homologous proteins were studied. ZCN8 stood out as only ZCN having requisite...
Alternative splicing plays a crucial role in plant development as well stress responses. Although alternative has been studied during and response to stress, the interplay between these two factors remains an open question. To assess effects of drought on developmentally regulated maize (Zea mays), 94 RNA-seq libraries from ear, tassel, leaf B73 public inbred line were constructed at four developmental stages under both well-watered conditions. This analysis was supplemented with publicly...
Abstract The phosphatidylethanolamine-binding proteins (PEBPs) represent an ancient protein family found across the biosphere. In animals they are known to act as kinase and serine protease inhibitors controlling cell growth differentiation. plants most extensively studied PEBP genes, Arabidopsis (Arabidopsis thaliana) FLOWERING LOCUS T (FT) TERMINAL FLOWER1 (TFL1) function, respectively, a promoter repressor of floral transition. Twenty-five maize (Zea mays) genes that encode PEBP-like...
DNA methylation can play important roles in the regulation of transposable elements and genes. A collection mutant alleles for 11 maize (Zea mays) genes predicted to controlling were isolated through forward- or reverse-genetic approaches. Low-coverage whole-genome bisulfite sequencing high-coverage sequence-capture applied lines determine context- locus-specific effects these mutations on profiles. Plants containing components RNA-directed pathway exhibit loss CHH at many loci as well CG...
Separation of the life cycle flowering plants into two distinct growth phases, vegetative and reproductive, is marked by floral transition. The initial inductive signals are perceived in leaves transmitted to shoot apex, where apical meristem restructured a reproductive meristem. In this study, we report cloning characterization maize (Zea mays) time gene delayed flowering1 (dlf1). Loss dlf1 function results late flowering, indicating required for timely promotion encodes protein with basic...
The transition from the vegetative to reproductive development is a critical event in plant life cycle. accurate prediction of flowering time elite germplasm important for decisions maize breeding programs and best agronomic practices. understanding genetic control has significantly advanced past decade. Through comparative genomics, mutant analysis, analysis QTL cloning, transgenic approaches, more than 30 candidate genes have been revealed relationships among these partially uncovered....
Increasing maize grain yield has been a major focus of both plant breeding and genetic engineering to meet the global demand for food, feed, industrial uses. We report that increasing extending expression MADS-box transcription factor gene, zmm28, under control moderate-constitutive promoter, results in plants with increased growth, photosynthesis capacity, nitrogen utilization. Molecular biochemical characterization zmm28 transgenic demonstrated their enhanced agronomic traits are...
Two maize genes with predicted translational similarity to the Arabidopsis FIE (Fertilization-Independent Endosperm) protein, a repressor of endosperm development in absence fertilization, were cloned and analyzed. Genomic sequences fie1 fie2 show significant homology within coding regions but none introns or 5' upstream. The gene is expressed exclusively developing kernels starting at approximately 6 days after pollination. an imprinted showing no detectable expression paternally derived...
We have taken a genomic approach to examine global gene expression in the maize endosperm relation dosage and parental effects. Endosperm of eight hybrids generated by reciprocal crosses their seven inbred parents were sampled at three developmental stages: 10, 14, 21 days after pollination (DAP). These samples subjected GeneCalling, an open-ended mRNA-profiling technology, which simultaneously analyzes thousands genes. Results indicated that overall level was dosage-dependent, is,...
Abstract TERMINAL FLOWER1 (TFL1)-like genes are highly conserved in plants and thought to function the maintenance of meristem indeterminacy. Recently, we described six maize (Zea mays) TFL1-related genes, named ZEA CENTRORADIALIS1 (ZCN1) ZCN6. To gain insight into their functions, generated transgenic overexpressing respective cDNAs driven by a constitutive promoter. Overall, ectopic expression TFL1-like produced similar phenotypes, including delayed flowering altered inflorescence...
The switch from vegetative to reproductive growth is marked by the termination of development and adoption floral identity shoot apical meristem (SAM). This process called transition. To elucidate molecular determinants involved in this process, we performed genome-wide RNA expression profiling on maize (Zea mays) apices at early stages using massively parallel signature sequencing technology. Profiling revealed significant up-regulation two MADS-box (ZMM) genes, ZMM4 ZMM15, after ZMM15 map...
Abstract Trimethylation of histone H3 Lys-27 (H3K27me3) plays a critical role in regulating gene expression during plant and animal development. We characterized the genome-wide distribution H3K27me3 five developmentally distinct tissues maize (Zea mays) plants two genetic backgrounds, B73 Mo17. There were more substantial differences profile between different than genotypes. The tissue-specific patterns often associated with among most imprinted genes that are expressed solely from paternal...
Background Plant diurnal rhythms are vital environmental adaptations to coordinate internal physiological responses alternating day-night cycles. A comprehensive view of biology has been lacking for maize (Zea mays), a major world crop. Methodology photosynthetic tissue, the leaf, and non-photosynthetic developing ear, were sampled under natural field conditions. Genome-wide transcript profiling was conducted on high-density 105 K Agilent microarray investigate rhythms. Conclusions In both...
Drought is a common abiotic stress which significantly limits global crop productivity. Maize an important staple and its yield determined by successful development of the female inflorescence, ear. We investigated drought responses across several developmental stages maize B73 inbred line under field conditions. suppressed plant growth, but had little impact on progression through stages. While ear growth was drought, process spikelet initiation not affected. Tassel reduced to lesser extent...
Polycomb group (PcG) proteins play an important role in developmental and epigenetic regulation of gene expression fruit fly (Drosophila melanogaster) mammals. Recent evidence has shown that Arabidopsis homologs PcG are also for the plant development. The objective this study was to characterize maize (Zea mays). 11 cloned from Enhancer zeste [E(z)], extra sex combs (esc), [E(Pc)] were used as queries perform TBLASTN searches against public expressed sequence tag database Pioneer Hi-Bred...
Maize originated as a tropical plant that required short days to transition from vegetative reproductive development. ZmCCT10 [CO, CONSTANS, CO-LIKE and TIMING OF CAB1 (CCT) transcription factor family] is regulator of photoperiod response was identified major QTL controlling sensitivity in maize. We modulated expression transgenic maize using two constitutive promoters with different levels. Transgenic plants over expressing either promoter were delayed their development but not affected...
Abstract We screened maize (Zea mays) cDNAs for sequences similar to the single myb-like DNA-binding domain of known telomeric complex proteins. identified, cloned, and sequenced five full-length representing a novel gene family, we describe analysis one them, Single myb histone 1 (Smh1). The Smh1 encodes small, basic protein with unique triple motif structure (a) an N-terminal SANT/myb-like homeodomain-like superfamily 3-helical-bundle-fold proteins, (b) central region homology conserved H1...
The transposable elements HeT-A and TART constitute the telomeres of Drosophila chromosomes. Both are non-long terminal repeat (LTR) retrotransposons, sharing remarkable property transposing only to chromosome ends. In addition, strong sequence similarity their gag proteins indicates that these coding regions share a common ancestor. These findings led assumption closely related. However, we now find produce quite different sets transcripts. produces sense-strand transcripts full-length...