A5068231850- Gene expression and cancer classification
- Molecular Biology Techniques and Applications
- Light effects on plants
- Plant Molecular Biology Research
- Circadian rhythm and melatonin
- Chromosomal and Genetic Variations
- Bioinformatics and Genomic Networks
- Labor Movements and Unions
- Epigenetics and DNA Methylation
- Genomics and Phylogenetic Studies
- Polysaccharides and Plant Cell Walls
- Genomics and Chromatin Dynamics
- Genetics, Aging, and Longevity in Model Organisms
- Big Data and Business Intelligence
- RNA and protein synthesis mechanisms
- Microbial Community Ecology and Physiology
- CRISPR and Genetic Engineering
- Renal and related cancers
- Migration, Identity, and Health
- Greenhouse Technology and Climate Control
- Tissue Engineering and Regenerative Medicine
- Pluripotent Stem Cells Research
- Genetics and Plant Breeding
- Social and Demographic Issues in Germany
- Glutathione Transferases and Polymorphisms
Jackson Laboratory
2021
University of Central Oklahoma
2019
SUNY Oneonta
2018
University of Rochester
2017
Pioneer Hi-Bred
2010-2016
Australian Research Council
2011
Australian Centre for Plant Functional Genomics
2011
DuPont (United States)
2011
Experimental Station
2011
The University of Melbourne
2011
The circadian clock is a molecular and cellular oscillator found in most mammalian tissues that regulates rhythmic physiology behavior. Numerous investigations have addressed the contribution of rhythmicity to cellular, organ, organismal physiology. We recently developed method look at transcriptional oscillations with unprecedented precision accuracy using high-density time sampling. Here, we report comparison oscillating transcription from mouse liver, NIH3T3, U2OS cells. Several...
Circadian rhythms of cell and organismal physiology are controlled by an autoregulatory transcription-translation feedback loop that regulates the expression rhythmic genes in a tissue-specific manner. Recent studies have suggested components circadian pacemaker, such as Clock Per2 gene products, regulate wide variety processes, including obesity, sensitization to cocaine, cancer susceptibility, morbidity chemotherapeutic agents. To identify more complete cohort transcriptionally regulated...
We have developed an approach to classify toxicants based upon their influence on profiles of mRNA transcripts. Changes in liver gene expression were examined after exposure mice 24 model treatments that fall into five well-studied toxicological categories: peroxisome proliferators, aryl hydrocarbon receptor agonists, noncoplanar polychlorinated biphenyls, inflammatory agents, and hypoxia-inducing agents. Analysis 1200 transcripts using both a correlation-based probabilistic resulted...
Naked mole rat (NMR) is a valuable model for aging and cancer research due to its exceptional longevity resistance. We observed that the reprogramming efficiency of NMR fibroblasts in response OSKM was drastically lower than mouse fibroblasts. Expression SV40 LargeT antigen (LT) dramatically improved Inactivation Rb alone, but not p53, sufficient improve efficiency, suggesting chromatin may be refractory reprogramming. Analysis global histone landscape revealed had higher levels repressive...
Molecular characterization of events is an integral part the advancement process during genetically modified (GM) crop product development. Assessment these traditionally accomplished by polymerase chain reaction (PCR) and Southern blot analyses. analysis can be time‐consuming comparatively expensive does not provide sequence‐level detail. We have developed a sequence‐based application, Southern‐by‐Sequencing (SbS), utilizing sequence capture coupled with next‐generation sequencing (NGS)...
Background Plant diurnal rhythms are vital environmental adaptations to coordinate internal physiological responses alternating day-night cycles. A comprehensive view of biology has been lacking for maize (Zea mays), a major world crop. Methodology photosynthetic tissue, the leaf, and non-photosynthetic developing ear, were sampled under natural field conditions. Genome-wide transcript profiling was conducted on high-density 105 K Agilent microarray investigate rhythms. Conclusions In both...
Abstract Background The elongating maize internode represents a useful system for following development of cell walls in vegetative cells the Poaceae family. Elongating internodes can be divided into four developmental zones, namely basal intercalary meristem, above which are found elongation, transition and maturation zones. Cells meristem elongation zones contain mainly primary walls, while secondary wall deposition accelerates zone predominates zone. Results major components cellulose,...
In both the suprachiasmatic nucleus (SCN) and peripheral tissues, circadian oscillator drives rhythmic transcription of downstream target genes. Recently, a number studies have used DNA microarrays to systematically identify oscillating transcripts in plants, fruit flies, rats, mice. These identified several dozen many hundred rhythmically expressed genes by sampling tissues every 4 hours for 1, 2, or more days. To extend this work, we performed microarray analysis on RNA derived from mouse...
Transcriptional profiling via microarrays holds great promise for toxicant classification and hazard prediction. Unfortunately, the use of different microarray platforms, protocols, informatics often hinders meaningful comparison transcriptional data across laboratories. One solution to this problem is provide a low-cost centralized resource that enables researchers share toxicogenomic has been generated on common platform. In an effort create such resource, we developed standardized set...
The MinION is a portable single-molecule DNA sequencing instrument that was released by Oxford Nanopore Technologies in 2014, producing long reads measuring changes ionic flow when single-stranded molecules translocate through the pores. While have an error rate substantially higher than ones produced short-read technologies, they can generate de novo assemblies of microbial genomes, after initial correction step includes alignment Illumina data or detection overlaps between to improve...
The environmental contaminant 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) serves as a prototype for range of toxicants and pharmacologic probe to study signal transduction by the aryl hydrocarbon receptor (AHR). Despite detailed understanding how TCDD exposure leads transcriptional up-regulation cytochrome P450-dependent monooxygenases, we know little about compounds like lead variety AHR-dependent toxic end points such liver pathology, terata, thymic involution, cancer. Using an acute...
Abstract Changes in cell wall polysaccharides, transcript abundance, metabolite profiles, and hormone concentrations were monitored the upper lower regions of maize (Zea mays) pulvini response to gravistimulation, during which plants placed a horizontal position returned vertical orientation. Heteroxylan levels increased pulvini, together with lignin, but xyloglucans heteromannan contents decreased. The degree substitution heteroxylan arabinofuranosyl residues decreased exhibited mechanical...
Profiling techniques such as microarrays, proteomics, and metabolomics are used widely to assess the overall effects of genetic background, environmental stimuli, growth stage, or transgene expression in plants. To potential regulatory use these agricultural biotechnology, we carried out microarray metabolomic studies 3 different tissues from 11 conventional maize varieties. We measured technical variations for both microarrays metabolomics, compared results individual plants corresponding...
Expression quantitative trait loci (eQTL) mapping can be used to identify the genetic variations that underlie inherited differences in gene transcription. We performed eQTL by combining whole genome transcriptional data from hypothalami of 33 strains inbred mice with a detailed haplotype map those same strains, revealing 10,655 trans associations and 31 cis eQTLs. One was found driven strain-specific variation expression Glutathione S-transferase, mu 5 (Gstm5). Gstm5 is one seven members...
The Union Internationale pour la Protection des Obtentions Végétales (UPOV) currently relies on morphological characteristics to evaluate distinctness, uniformity, and stability (DUS) as eligibility requirements for the granting of Plant Variety (PVP). We used 10 maize ( Zea mays L.) inbred lines, including both unrelated closely similar pairs, representing three heterotic groups compare abilities morphological, ribonucleic acid (RNA) transcription, metabolomic, single nucleotide...
The use of next-generation DNA sequencing technologies has greatly facilitated reference-guided variant detection in complex plant genomes. However, complications may arise when regions adjacent to a read interest are used for marker assay development, or reference sequences incomplete, as short reads alone not be long enough ascertain their uniqueness. Here, the possibility generating longer discrete large and genome maize is demonstrated, using modified version paired-end RAD library...
Purpose The purposes of this paper are as follows: first analyze and visualize the price-changing pattern common electronic resources; second, provide predictions for future price changes at vendor level; third, discover any potential cause such changes; fourth assess practice skills techniques used statistical analysis data visualization. Design/methodology/approach Statistical visualization library’s expenditure were conducted using business intelligence tools, in case, Microsoft Excel IBM...