A5010647834- RNA modifications and cancer
- Immunotherapy and Immune Responses
- Genetics and Physical Performance
- interferon and immune responses
- Cardiomyopathy and Myosin Studies
- Mechanical Circulatory Support Devices
- Epigenetics and DNA Methylation
- Hemoglobinopathies and Related Disorders
- Sports Performance and Training
- Immune Cell Function and Interaction
- Cardiovascular Effects of Exercise
- Genomics and Chromatin Dynamics
- Cardiac Arrest and Resuscitation
- Phonocardiography and Auscultation Techniques
- CAR-T cell therapy research
- Hearing, Cochlea, Tinnitus, Genetics
- Eosinophilic Disorders and Syndromes
- Immune Response and Inflammation
- IL-33, ST2, and ILC Pathways
- Exercise and Physiological Responses
- Acute Myocardial Infarction Research
- Asthma and respiratory diseases
- Advanced Battery Technologies Research
- Biochemical Analysis and Sensing Techniques
- Coronary Interventions and Diagnostics
University of Iowa
2024
UNSW Sydney
2017-2024
University of Minnesota
2021-2022
Temple University
2022
Atlanta VA Medical Center
2018
Emory University
2018
The University of Sydney
2016
University of Maryland, Baltimore
2015
Sensorineural hearing loss is a common and currently irreversible disorder, because mammalian hair cells (HCs) do not regenerate current stem cell gene delivery protocols result only in immature HC-like cells. Importantly, although the transcriptional regulators of embryonic HC development have been described, little known about postnatal maturating HCs. Here we apply type-specific functional genomic analysis to transcriptomes auditory vestibular sensory epithelia from early mice. We...
Abstract Establishing the magnitude and kinetics of polyclonal Ag-specific CD8 T-cell responses, in addition to their functional fitness, is critical for evaluating a host’s ability respond different kinds infections and/or immunizations. To track responses during infection, surrogate-activation-marker approach (CD8αloCD11ahi) used distinguish naïve Ag-experienced effector/memory T cells vivo. However, semidifferentiated virtual memory (Tvm) have recently been identified...
Bacterial products such as lipopolysaccharides (or endotoxin) cause systemic inflammation, resulting in a substantial global health burden. The onset, progression, and resolution of the inflammatory response to endotoxin are usually tightly controlled avoid chronic inflammation. Members NF-κB family transcription factors key drivers inflammation that activate sets genes signals. Such responses typically short-lived can be suppressed by proteins act post-translationally, SOCS (suppressor...
Abstract There is a significant interest in designing therapeutic agents that can enhance ADCC and thereby improve clinical responses with approved antibodies. We recently reported the combination of an imidazoquinoline-based TLR7/8 agonist (522) monoclonal antibody improved vitro vivo. In present study, we tested several new small molecule agonists induce significantly higher cytokines compared to both FDA-approved TLR7 agonist, imiquimod, 522. evaluated these therapy, main goal enhancing...
Immunostimulatory adjuvants that potently activate antigen-presenting cells and (in turn) prime cytotoxic T are a key component of anticancer vaccines. In this study, we investigated multi-adjuvant approach combining TLR 7/8 agonist (522) STING (DMXAA) to promote enhanced antigen cross-presentation, stimulate specific antitumor T-cell responses, provide improved efficacy. vitro experiments using bone marrow-derived dendritic (BMDCs) confirmed activation with the 522-DMXAA combination based...
Eosinophils are granular leukocytes of the innate immune system that play important functions in host defense. Inappropriate activation eosinophils can occur pathologies such as asthma and esophagitis. However, also reside within adipose tissue, where they homeostatic roles thermogenic beige fat. Here we performed bulk RNA sequencing mouse tissue-resident isolated from both subcutaneous gonadal depots, for first time, compared gene expression to blood eosinophils. We found a predominantly...
α-Actinin-3 deficiency alters muscle mass regulation and protects against dexamethasone-induced wasting.
Abstract Development of Plasmodium-specific humoral immunity is critically dependent on CD4 Th cell responses and germinal center (GC) reactions during blood-stage Plasmodium infection. IL-21, a cytokine primarily produced by T cells, an essential regulator affinity maturation, isotype class-switching, B differentiation, maintenance GC in response to many infection immunization models. In models experimental malaria, mice deficient IL-21 or its receptor IL-21R fail develop memory populations...
We have developed Förster resonance energy transfer (FRET)-based experiments for measuring the binding affinity, off-rates, and inferred on-rates interactions between a family of transcriptional regulators their intrinsically disordered partners. It was difficult to evaluate these previously, as are obligate proteins that aggregate in absence partner. The assays rely on fusion constructs where domains linked by flexible tether containing specific protease site, with fluorescent at either end...
Summary The eukaryotic ribosome is highly modified by protein methylation, yet many of the responsible methyltransferases remain unknown. Here we have identified SMYD5 as a ribosomal methyltransferase that catalyses trimethylation RPL40/eL40 at lysine 22. Through systematic mass spectrometry-based approach, show human has 12 primary sites including RPL40 K22. in vitro methylation synthetic using fractionated lysate, then identify candidate K22 methyltransferase. We recombinant robust...
Hemoglobin switching is a complex biological process not yet fully elucidated. The mechanism regulating the suppression of fetal hemoglobin (HbF) expression particular interest because positive impact HbF on course diseases such as β-thalassemia and sickle cell disease, hereditary disorders that affect health countless individuals worldwide. Several transcription factors have been implicated in control HbF, which BCL11A has emerged major player silencing. SOX6 also silencing critical to...
Abstract We have developed Förster resonance energy transfer (FRET)‐based experiments for measuring the binding affinity, off‐rates, and inferred on‐rates interactions between a family of transcriptional regulators their intrinsically disordered partners. It was difficult to evaluate these previously, as are obligate proteins that aggregate in absence partner. The assays rely on fusion constructs where domains linked by flexible tether containing specific protease site, with fluorescent at...
Abstract Homozygosity for the common ACTN3 null polymorphism ( 577X) results in α-actinin-3 deficiency ~20% of humans worldwide and is linked to reduced sprint power performance both elite athletes general population. α-Actinin-3 also associated with muscle mass strength, increased risk sarcopenia elderly, altered response wasting induced by denervation immobilisation. genotype a disease modifier Duchenne muscular dystrophy (DMD), slower progression. Here we show that plays key role...
Abstract Various classes of molecules including toll-like receptor (TLR) agonists, exosomes, and metallo compounds have been evaluated as adjuvants for cancer vaccines. However, their wide-spread clinical use has limited by transient immune responses serious side-effects. Here, we propose the a multi-adjuvant approach that combines two different adjuvants, STING TLR 7/8 based on distinct cell targets, signalling pathways, significant roles in activation maintenance responses. We potential...