A5054014646- Cell death mechanisms and regulation
- Mechanisms of cancer metastasis
- Melanoma and MAPK Pathways
- Gut microbiota and health
- Cancer-related molecular mechanisms research
- Hearing, Cochlea, Tinnitus, Genetics
- Clostridium difficile and Clostridium perfringens research
- Graphene and Nanomaterials Applications
- Prostate Cancer Treatment and Research
- Lung Cancer Treatments and Mutations
- Hearing Loss and Rehabilitation
- Helicobacter pylori-related gastroenterology studies
- Cancer Mechanisms and Therapy
- Lymphoma Diagnosis and Treatment
- Cancer Genomics and Diagnostics
- Lung Cancer Research Studies
- Single-cell and spatial transcriptomics
- Mycobacterium research and diagnosis
- Microtubule and mitosis dynamics
- Radiomics and Machine Learning in Medical Imaging
- Bone Tissue Engineering Materials
- MicroRNA in disease regulation
- Cancer Immunotherapy and Biomarkers
- Advanced biosensing and bioanalysis techniques
- Cancer, Lipids, and Metabolism
University of Maryland, Baltimore
2016-2025
Jilin Agricultural University
2021-2025
Siemens (China)
2025
Dalian University of Technology
2024-2025
Hunan Agricultural University
2024-2025
BOE Technology Group (China)
2023-2025
Jilin University
2025
Inner Mongolia Agricultural University
2023-2024
First Affiliated Hospital of Hebei Medical University
2024
Guangzhou University of Chinese Medicine
2023-2024
Clostridium difficile causes antibiotic-associated diarrhea and pseudomembraneous colitis is responsible for a large increasing fraction of hospital-acquired infections. Fecal microbiota transplantation (FMT) an alternate treatment option recurrent C. infection (RCDI) refractory to antibiotic therapy. It has recently been discussed favorably in the clinical scientific communities receiving public attention. However, short- long-term health consequences FMT remain concern, as effects...
Sensorineural hearing loss is a common and currently irreversible disorder, because mammalian hair cells (HCs) do not regenerate current stem cell gene delivery protocols result only in immature HC-like cells. Importantly, although the transcriptional regulators of embryonic HC development have been described, little known about postnatal maturating HCs. Here we apply type-specific functional genomic analysis to transcriptomes auditory vestibular sensory epithelia from early mice. We...
Abstract Intellectual disability (ID) is a clinically and genetically heterogeneous disorder, affecting 1–3% of the general population. Although research into genetic causes ID has recently gained momentum, identification pathogenic mutations that cause autosomal recessive (ARID) lagged behind, predominantly due to non-availability sizeable families. Here we present results exome sequencing in 121 large consanguineous Pakistani In 60 families, identified homozygous or compound heterozygous...
Noise-induced hearing loss (NIHL) is the most prevalent form of acquired and affects about 40 million US adults. Among suggested therapeutics tested in rodents, suberoylanilide hydroxamic acid (SAHA) has been shown to be otoprotective from NIHL; however, these results were limited male mice. Here we effect SAHA on 10-week-old B6CBAF1/J mice both sexes, which exposed 2 h octave-band noise (101 dB SPL centered at 11.3 kHz). Hearing was assessed by measuring auditory brainstem responses (ABR)...
Noise-induced hearing loss (NIHL) results from a complex interplay of damage to the sensory cells inner ear, dysfunction its lateral wall, axonal retraction type 1C spiral ganglion neurons, and activation immune response. We use RiboTag single-cell RNA sequencing survey cell-type-specific molecular landscape mouse ear before after noise trauma. identify induction transcription factors STAT3 IRF7 immune-related genes across all cell-types. Yet, transcriptomic changes dominate The ATF3/ATF4...
Abstract Background Multi‐omics features of cell‐free DNA (cfDNA) can effectively improve the performance non‐invasive early diagnosis and prognosis cancer. However, multimodal characterization cfDNA remains technically challenging. Methods We developed a comprehensive multi‐omics solution (COMOS) to specifically obtain an extensive fragmentomics landscape, presented by breakpoint characteristics nucleosomes, CpG islands, DNase clusters enhancers, besides typical methylation, copy number...
The incidence of autoimmune diseases such as systemic lupus erythematosus, rheumatoid arthritis, and primary biliary cirrhosis has increased significantly in China. Information about the susceptibility or potential general population is lacking. To explore prevalence antinuclear antibody (ANA) its specificities Twenty thousand nine hundred seventy sera samples were taken from physical examination center Baoding, Indirect immunofluorescence line immunoassays used to detect ANA specificities,...
Dynamic interactions between the host and gastrointestinal microbiota play an important role for local systemic immune homeostasis. Helminthic parasites modulate response, resulting in protection against autoimmune disease but also increased susceptibility to pathogen infection. The underlying mechanisms remain largely unknown. We showed that type 2 response enteric Nippostrongylus brasiliensis infection mice was associated with altered intestinal mucin AMP expression shifts composition....
Abstract Early diagnosis of primary central nervous system lymphoma (PCNSL) represents a challenge, and cerebrospinal fluid (CSF) cytokines may be diagnostic biomarkers for PCNSL. We used an electrochemiluminescence immunoassay to measure interleukin (IL)-10, IL-6, IL-8 tumor necrosis factor α (TNF-α) in the CSF 22 B cell PCNSL patients 80 with other CNS diseases. IL-10 was significantly higher than control group (median 74.7 pg/ml vs < 5.0 pg/ml, P 0.000). Using cutoff value 8.2...
Increasing morbidity and mortality from Clostridium difficile infection (CDI) present an enormous challenge to healthcare systems. express type IV pili (T4P), but their function remains unclear. Many chronic recurrent bacterial infections result biofilms, surface-associated communities embedded in extracellular matrix. CDI may be biofilm mediated; T4P are important for formation a number of organisms. We evaluate the role C. using RNA sequencing, mutagenesis complementation gene encoding...
Cell type-specific ribosome-pulldown has become an increasingly popular method for analysis of gene expression. It allows expression from intact tissues and monitoring protein synthesis in vivo. However, while its utility been assessed, technical aspects related to sequencing these samples, often starting with a smaller amount RNA, have not reported. In this study, we evaluated the performance five library prep protocols ribosome-associated mRNAs when only 250 pg-4 ng total RNA are used. We...
Abstract Studies of developmental and functional biology largely rely on conditional expression genes in a cell type-specific manner. Therefore, the importance specificity lack inherent phenotypes for Cre- driver animals cannot be overemphasized. The Gfi1 Cre mouse is commonly used hair cell-specific gene deletion/reporter activation inner ear. Here, using immunofluorescence flow cytometry, we show that mice produce pattern recombination not strictly limited to cells within We observe broad...
ABSTRACT Despite the known importance of transcription factors ATOH1, POU4F3 and GFI1 in hair cell development regeneration, their downstream transcriptional cascades inner ear remain largely unknown. Here, we have used Gfi1cre;RiboTag mice to evaluate changes translatome absence GFI1. We identify a systematic downregulation differentiation genes, concomitant with robust upregulation neuronal genes GFI1-deficient cells. This includes increased expression neuronal-associated (e.g. Pou4f1) as...
Given the heightened interest in manipulation of co-signaling cascades for cancer immunotherapy, we sought to determine how/whether tumors decorated with therapeutic monoclonal antibodies (mAbs) impact expression molecules on human NK cells. Stimulation cells aggregated IgG1 resulted upregulation HAVCR2 - gene encoding T-cell immunoglobulin and mucin-containing domain (Tim)-3 known be involved induction peripheral T cell tolerance. This was recapitulated at protein level, following...
Abstract β‐Carotene displays antioxidant and anti‐inflammatory activities prevents the development of cancer. Ulcerative colitis (UC) is a kind inflammatory bowel disease that accompanied by certain risk colon However, role β‐carotene in modulation gut microbiota UC improvement unclear. In this research, properties on composition were evaluated rat model induced dextran sulfate sodium (DSS). The results revealed significantly ( p < 0.05) decreased severity rats, as assessed using body...
Abstract Background Nutrition drives immunity and health in animals, maternal benefits offspring. In our previous study, a nutritional intervention strategy was found to promote the of hens, which subsequently improved growth offspring chicks. Maternal effects clearly exist, but how are mothers’ immune advantages transferred their offspring, do they benefit them? Results Here, we traced beneficial back process egg formation reproductive system, focused on embryonic intestinal transcriptome...
Re-irradiation (re-RT) for recurrent head and neck cancer (rHNC) is challenging. We describe clinical outcomes toxicity of proton therapy (PT) HNC, report genomic alterations associated with patterns failure. performed a retrospective analysis rHNC patients treated PT. Outcomes were estimated using the Kaplan-Meier method. Univariate (UVA) multivariate analyses (MVA) to assess multiple patient factors. Next-generation sequencing on available samples. Eighty-nine PBS-PT median follow-up 12 mo...