A5012027313- Cancer-related gene regulation
- Epigenetics and DNA Methylation
- Chronic Myeloid Leukemia Treatments
- CRISPR and Genetic Engineering
- RNA modifications and cancer
- Hemoglobinopathies and Related Disorders
- Chronic Lymphocytic Leukemia Research
- Acute Myeloid Leukemia Research
- Blood groups and transfusion
- Prenatal Screening and Diagnostics
- Genomics and Chromatin Dynamics
- Iron Metabolism and Disorders
- Chromosomal and Genetic Variations
- Platelet Disorders and Treatments
- Angiogenesis and VEGF in Cancer
- Immune Cell Function and Interaction
- Microtubule and mitosis dynamics
- Virus-based gene therapy research
- Lymphatic System and Diseases
- Glycosylation and Glycoproteins Research
- Renal Diseases and Glomerulopathies
- Galectins and Cancer Biology
- DNA Repair Mechanisms
- Biochemical and Molecular Research
- PI3K/AKT/mTOR signaling in cancer
Yale University
2009-2022
Harvard University
2013-2016
Boston Children's Hospital
2013-2016
Dana-Farber Cancer Institute
2015-2016
Harvard Stem Cell Institute
2015-2016
Harvard University Press
2013
Institute of Cell Biology
2012
Genome-wide association studies (GWASs) have ascertained numerous trait-associated common genetic variants, frequently localized to regulatory DNA. We found that variation at BCL11A associated with fetal hemoglobin (HbF) level lies in noncoding sequences decorated by an erythroid enhancer chromatin signature. Fine-mapping uncovers a motif-disrupting variant reduced transcription factor (TF) binding, modestly diminished expression, and elevated HbF. The surrounding function vivo as...
Tyrosine kinase inhibitors (TKI) have revolutionized chronic myelogenous leukemia (CML) management. Disease eradication, however, is hampered by innate resistance of leukemia-initiating cells (LIC) to TKI-induced killing, which also provides the basis for subsequent emergence TKI-resistant mutants. We report that EZH2, catalytic subunit Polycomb Repressive Complex 2 (PRC2), overexpressed in CML LICs and required colony formation survival cell-cycle progression cell lines. A critical role...
Lymphatic vessels (LVs) are important structures for antigen presentation, lipid metabolism, and as conduits tumor metastases, but they have been difficult to visualize in vivo. Prox1 is a transcription factor that necessary lymphangiogenesis ontogeny the maintenance of LVs. To LVs lymph node living mouse real time, we made ProxTom transgenic C57BL/6 background using red fluorescent suitable vivo imaging. The transgene contained all regulatory sequences was faithfully expressed coincident...
DNA polymerase beta (pol beta) is the key gap-filling in base excision repair, repair pathway responsible for repairing up to 20000 endogenous lesions per cell day. Pol also widely used as a model structure and function studies, several structural regions have been identified being critical fidelity of enzyme. One these hydrophobic hinge, network residues located between palm fingers subdomains. Previous work by our lab has shown that hinge Y265, I260, F272 are functioning discrimination...
<p>Figure showing Ezh2 is required for initiation and development of CML in mouse.</p>
<p>Gene expression analysis.</p>
<p>Supplementary Figure Legends</p>
<p>Figure showing expression of EZH2 in CML and sensitivity cells to inhibition.</p>
<p>Sensitivity of CML cells to EZH2 inhibition.</p>
<p>In vitro validation of Ezh2 gRNA and its impact on CML.</p>
<p>Sensitivity of CML cells to EZH2 inhibition.</p>