A5031405834- Neurogenesis and neuroplasticity mechanisms
- Neuroscience and Neuropharmacology Research
- Axon Guidance and Neuronal Signaling
- CRISPR and Genetic Engineering
- Single-cell and spatial transcriptomics
- Pluripotent Stem Cells Research
- Retinal Development and Disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- Ocular Oncology and Treatments
- Genomics and Chromatin Dynamics
- Blood groups and transfusion
- Retinal and Macular Surgery
- Multiple Myeloma Research and Treatments
- Nuclear Receptors and Signaling
- Neural dynamics and brain function
- Microtubule and mitosis dynamics
- RNA Research and Splicing
- Retinal Diseases and Treatments
- Identification and Quantification in Food
- Microbial Inactivation Methods
- Hereditary Neurological Disorders
- Hemoglobinopathies and Related Disorders
- Erythrocyte Function and Pathophysiology
- Gene Regulatory Network Analysis
- Corneal Surgery and Treatments
Children's Hospital of Los Angeles
2024
Harvard University
2012-2024
University of California, San Francisco
2024
Harvard Stem Cell Institute
2016-2023
Stanford University
2021-2023
Smith-Kettlewell Eye Research Institute
2022-2023
University of Southern California
2022-2023
Palo Alto University
2022
Developing corticospinal axons specifically
Specific and highly diverse connectivity between functionally specialized regions of the nervous system is controlled at multiple scales, from anatomically organized following macroscopic axon tracts to individual target-finding synapse formation. Identifying mechanisms that enable entire subpopulations related neurons project their axons with regional specificity within stereotyped form appropriate long-range key understanding brain development, organization, function. Here, we investigate...
We describe a binary expression aleatory mosaic (BEAM) system, which relies on DNA delivery by transfection or viral transduction along with nested recombinase activity to generate two genetically distinct, non-overlapping populations of cells for comparative analysis. Control labeled red fluorescent protein (RFP) can be directly compared experimental manipulated genetic gain loss function and GFP. Importantly, BEAM incorporates recombinase-dependent signal amplification delayed reporter...
Precise, area-specific connectivity of interhemispheric callosal projection neurons (CPN) in the cerebral cortex is critical for sensory, associative, and behavioral functions. CPN circuitry, which connects integrates hemispheres via corpus callosum, centrally implicated autism spectrum disorder (ASD) intellectual disability (ID). Though transcriptional controls regulating subtype areal development are partially understood, downstream subcellular molecular machinery that implements circuitry...
Surgical placement of eye plaque brachytherapy (EPB) is the standard care for treatment uveal melanomas, including iris/iridociliary melanomas. However, unique challenges exist in anterior EPB placement. Here, we describe a surgical technique when requires positioning onto cornea. Blunt conjunctival peritomy exposes sclera overlying tumor. A “dummy” placed, with confirmed by direct visualization. The amniotic membrane draped across cornea and anchored eyelet sutures, placed membrane,...
SUMMARY Both cell-intrinsic competency and extracellular cues regulate axon projection, but mechanisms that coordinate these elements remain poorly understood. Subcerebral projection neurons (SCPN) extend their primary axons from cortex through subcortical structures, including the striatum, targeting brainstem spinal cord. We identify transcription factor Bcl11b/Ctip2 functions in multiple independent neuron populations to control SCPN development. Bcl11b expressed by is required...
Genetic mosaic analysis, in which mutant cells reside intermingled with wild-type cells, is a powerful experimental approach, but has not been widely used mice because existing genome-based strategies require complicated and protracted breeding schemes. We have developed an alternative approach termed BEAM (for Binary Expression Aleatory Mosaic) that relies on sparse recombinase activation to generate two genetically distinct, non-overlapping populations of for comparative analysis....