The relationship between the ATP content of mature human oocytes and developmental potential after uterine transfer sibling embryos was examined in 20 non-male factor in-vitro fertilization (IVF) patients matched for age, fertility history, ovarian stimulation protocol, oocyte quality number, stage morphology at transfer. determined uninseminated unfertilized each cohort, that developed dispermic or which showed significant fragmentation. effect reduced on meiotic maturation, preimplantation...

The developmental competence of mouse and human early embryos appears to be directly related the metabolic capacity a finite complement maternally inherited mitochondria that appear begin replicate after implantation. Mitochondrial dysfunctions resulting from variety intrinsic extrinsic influences, including genetic abnormalities, hypoxia oxidative stress, can profoundly influence level ATP generation in oocytes embryos, which turn may result aberrant chromosomal segregation or arrest....

Abstract This study examined the effects of cryopreservation on cellular organization, chromosomal complement, and developmental potential immature mature mouse human oocytes. Chromosomal analyses were performed by DNA fluorescence microscopy karyotyping same metaphase II‐stage oocytes before after freezing. Cellular involved electron microscopy, time‐lapse video recording, fluorescent‐probe cortical granules. The findings demonstrate that while profound cytoplasmic, nuclear, nucleolar...

The occurrence of a pleiomorphic population cytoplasmic fragments is common characteristic early human embryos fertilized in vitro. Here, temporal, spatial, fine structural, and biochemical aspects fragmentation were examined fragmented monospermic dispermic pronuclear to cleavage stages classified as stage-appropriate during the first 3.5 days culture. morphodynamics certain patterns movement composition analysed by time-lapse video, mitochondrial fluorescent probes, transmission electron...

The magnitude of the potential difference (polarity) across inner mitochondrial membrane (DeltaPsim) determines levels several activities, including ATP generation, focal regulate calcium homeostasis and organelle volume homeostasis. We investigated whether a domain mitochondria in mouse oocyte, characterized by high DeltaPsim unique location subplasmalemmal cytoplasm, is involved earliest events fertilization: sperm attachment, penetration cortical granule exocytosis. Experimental...

Abstract Learned fear and safety are associated with distinct oscillatory states in the basolateral amygdala (BLA) medial prefrontal cortex (mPFC). To determine if how these network support retrieval of competing memories, we mimicked endogenous activity through optogenetic stimulation parvalbumin-expressing interneurons mice during contextual extinction memories. We found that exogenously induced 4 Hz 8 BLA exerts bi-directional control over conditioned freezing behavior an experience-...

Abstract This report describes spatial and temporal aspects of sperm penetration intracytoplasmic migration, pronuclear evolution the specificity presyngamic opposition, stage-specific changes in cytoskeletal organization relative contribution maternal paternal components to mitotic spindle formation. These studies involved observations living human oocytes during conventional insemination vitro after deposition spermatozoa, analysis chromatin distribution evolution, detection actin α-, rβ-...

Ten patients are presented with tumours complying the criteria established by World Health Organization for `mixed of lung9. A slowly growing intrabronchial neoplasm indistinguishable from a pleomorphic adenoma (mixed salivary tumour) and peripheral circumscribed tumour most features chondromatous hamartoma were considered benign. Of eight malignant neoplasms two regarded as `pulmonary blastomas9, one benign epithelial tubular component other cytological evidence malignancy in epithelium;...

This study determined the occurrence of two molecular markers apoptosis, chromosomal DNA strand breaks and oolemma phosphatidylserine redistribution, in >200 uninseminated unfertilized human oocytes, >800 newly ovulated cultured mouse oocytes. were analysed by terminal deoxynucleotidyl transferase-mediated dUDP nick-end labelling (TUNEL) annexin V staining, with imaging conventional epifluorescence scanning laser confocal fluorescence microscopy. More than 300 intact 500 fragmented oocytes...

Studies of mitochondria in mouse and human oocytes preimplantation stage embryos have focused primarily on their metabolic capacity to generate ATP. However, it is becoming increasingly apparent that are also regulatory agents other processes involved the establishment developmental competence, including calcium homeostasis apoptosis. The magnitude inner mitochondrial membrane potential, or its polarity (ΔΨm), a physiochemical property related levels organelle activity, differences spatial...

Journal Article Evolution of the sperm aster after microinjection isolated human centrosomes into meiotically mature oocytes Get access Jonathan Van Blerkom, Blerkom 1 Department Molecular, Cellular and Developmental Biology, University ColoradoBoulder, CO 80309Reproductive Genetics In Vitro, DenverCO 80222, USA To whom correspondence should be addressed Search for other works by this author on: Oxford Academic PubMed Google Scholar Patrick Davis Human Reproduction, Volume 10, Issue 8,...

A total of 518 normal-appearing, meiotically mature human oocytes that were judged unfertilized after insemination in vitro examined for sperm penetration by conventional fluorescence and laser scanning confocal microscopy with DNA-specific probes. similar analysis was performed on 29 single pronuclear presumed to originate spontaneous (parthenogenetic) activation. The results demonstrate 22% the 52% parthenogenetic actually penetrated. Sperm occurred both normozoospermic male factor cases....

Specific and highly diverse connectivity between functionally specialized regions of the nervous system is controlled at multiple scales, from anatomically organized following macroscopic axon tracts to individual target-finding synapse formation. Identifying mechanisms that enable entire subpopulations related neurons project their axons with regional specificity within stereotyped form appropriate long-range key understanding brain development, organization, function. Here, we investigate...

Journal Article The developmental ability of human oocytes penetrated at the germinal vesicle stage after insemination in vitro Get access Jonathan Van Blerkom, Blerkom 3 1Department Molecular, Cellular and Developmental Biology, University ColoradoBoulder, CO 80309, USA2Reproductive Genetics In VitroDenver, 80222, USA 3To whom correspondence should be addressed at: Department Colorado, Boulder, Search for other works by this author on: Oxford Academic PubMed Google Scholar Patrick W. Davis,...

In a randomized trial of wound closure in 512 abdominal wounds, wounds were closed with either reinforced Steristrip skin closures or interrupted silk sutures. Comparisons made pain and discomfort, infection, discharge, redness, width, reaction. The causes peeling the tapes assessed. results showed that significantly more comfortable patients preferred them to sutures (P less than 0.01), but wide scars occurred often. There was no difference rates infection case allergy seen. Closure by...

Abstract Experience with 38 cases of Hirschsprung's disease seen over a 20-year period is described. Only 24 per cent presented combination symptoms and signs such as to suggest the correct diagnosis on clinical grounds. Barium-enema examination carries high ‘true-positive’ rate, but reported negative must be viewed critically. A radiological idiopathic megacolon should not accepted without supplementary rectal biopsy and/or anorectal pressure studies. Survival surgery compatible disease,...