A5039853884- Virus-based gene therapy research
- Pancreatic function and diabetes
- Protein Degradation and Inhibitors
- Estrogen and related hormone effects
- Bacteriophages and microbial interactions
- Pregnancy and preeclampsia studies
- Platelet Disorders and Treatments
- Polyomavirus and related diseases
- Metabolism, Diabetes, and Cancer
- Retinoids in leukemia and cellular processes
- Viral Infectious Diseases and Gene Expression in Insects
- Ubiquitin and proteasome pathways
- Gastrointestinal Bleeding Diagnosis and Treatment
- Reproductive System and Pregnancy
- Adipose Tissue and Metabolism
- Peroxisome Proliferator-Activated Receptors
- Receptor Mechanisms and Signaling
- RNA Research and Splicing
- Olfactory and Sensory Function Studies
- Cancer, Hypoxia, and Metabolism
- Renal Transplantation Outcomes and Treatments
- Liver physiology and pathology
- RNA Interference and Gene Delivery
- Viral gastroenteritis research and epidemiology
- Vitamin K Research Studies
Tokai University
2024
National Center for Geriatrics and Gerontology
2011-2023
Nagoya Institute of Technology
2022
Tokyo Institute of Technology
1995-2013
Nihon University
1998-2013
Osaka University
2013
Kyoto Prefectural University of Medicine
2013
National Center for Global Health and Medicine
2013
Keio University
2012
Tokyo Medical and Dental University
2012
Tadashi Wada, Toshiyuki Takagi, Yuki Yamaguchi, Anwarul Ferdous, Takeshi Imai, Susumu Hirose, Seiji Sugimoto, Keiichi Yano, Grant A. Hartzog, Fred Winston, Stephen Buratowski, and Hiroshi Handa Faculty of Bioscience Biotechnology, Tokyo Institute Technology, Midori-ku, Yokohama 226, Japan; National Genetics, Mishima, Shizuoka-ken 411, Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., Machida-shi 194, Department Genetics Biological Chemistry Molecular Pharmacology, Harvard Medical School,...
The peroxisome proliferator-activated receptor γ (PPARγ) mediates the activity of insulin-sensitizing thiazolidinediones and plays an important role in adipocyte differentiation fat accretion. analysis PPARγ functions mature adipocytes is precluded by lethality –/– fetuses tetraploid-rescued pups. Therefore we have selectively ablated adult mice using tamoxifen-dependent Cre-ER T2 recombination system. We show that PPARγ-null white brown die within a few days are replaced newly formed...
Retinoid X receptor α (RXRα) is involved in multiple signaling pathways, as a heterodimeric partner of several nuclear receptors. To investigate its function energy homeostasis, we have selectively ablated the RXRα gene adipocytes 4-week-old transgenic mice by using tamoxifen-inducible Cre-ER T2 recombination system. Mice lacking were resistant to dietary and chemically induced obesity impaired fasting-induced lipolysis. Our results also indicate that adipocyte differentiation. Thus, our...
Abstract bor1–1 (high boron requiring), an Arabidopsis thaliana mutant that requires a high level of B, was isolated. When the B concentration in medium reduced to 3 [mu]M, expansion rosette leaves severely affected but not wild-type plants. In containing 30 [mu]M grew normally showed female sterility, whereas wild type able set seeds. These defects were detected with supplementation 100 B. vivo concentrations mutants lower than those type, especially inflorescence stems. Tracer experiments...
Multiprotein bridging factor 1 (MBF1) is a coactivator which mediates transcriptional activation by interconnecting the general transcription TATA element-binding protein and gene-specific activators such as <i>Drosophila</i>nuclear receptor FTZ-F1 or yeast basic leucine zipper GCN4. The human homolog of MBF1 (hMBF1) has been identified but its function, especially in transcription, remains unclear. Here we report cDNA cloning functional analysis hMBF1. Two isoforms, term hMBF1α hMBF1β, have...
Transcription factor IIA has been shown to interact with the TATA-binding protein and act early during preinitiation complex formation. The human is composed of three subunits (alpha, beta, gamma). A cDNA clone encoding largest subunit TFIIA (alpha) was isolated. recombinant alpha polypeptide, together beta gamma subunits, capable reconstituting activity. Studies using antibodies raised against polypeptide demonstrate that can be an integral component complex. We not only interacts TBP but...
Silk gland factor-1 (SGF-1) regulates transcription of the Bombyx sericin-1 gene via interaction with SA site. In this study, two related SGF-1 polypeptides apparent molecular masses 40 and 41 kDa were purified. Specific these proteins site was demonstrated by electrophoretic mobility shift dimethyl sulfate methylation interference assays. The 40-kDa protein partially sequenced characterized as a new member fork head/HNF-3 family. Several full-length cDNAs encoding possibly also 41-kDa...
Retinoid X receptors (RXRs) are involved in a number of signaling pathways as heterodimeric partners numerous nuclear receptors. Hepatocytes express high levels the RXRα isotype, well several its putative partners. Germ-line disruption (knockout) has been shown to be lethal utero , thus precluding analysis function at later life stages. Hepatocyte-specific during liver organogenesis recently revealed that presence hepatocytes is not mandatory for mouse, least under normal mouse facility...
Estrogens play pivotal roles in sexual development, growth, reproduction, and sex differentiation have been implicated a number of physiological processes various tissues. Most the effects estrogens are mediated by estrogen receptors alpha ( ER α), beta β), G protein‐coupled receptor 30 GPR 30). The liver is known to be target tissue for signaling, but role this signaling not well characterized. Through analyses an estradiol (E2)‐treated hepatocyte cell line mice, we showed that E2 controls...
Two kinds of trans-acting factors that regulate transcription from the promoter adenovirus early-region 4 (E4) have been identified by reconstituting nuclear extracts HeLa cells. They were designated E4TF1 and E4TF3 for E4 factors. These responsible efficient accurate in vitro promoter, as another factor, E4TF2, a crude fraction containing endogenous RNA polymerase II. stimulated but not major late or mutant lacking E4TF1-binding site. Footprint analysis revealed it binds to specific region,...
Vitamin K2 (VK2, menaquinone) is known to have anticancer activity in vitro and vivo. Although its effect thought be mediated, at least part, by the induction of apoptosis, underlying molecular mechanism remains elusive. Here, we identified Bcl-2 antagonist killer 1 (Bak) as a target VK2-induced apoptosis. VK2 directly interacts with Bak induces mitochondrial-mediated Bcl-2-associated X protein (Bax), another member family, are generally functionally redundant, only necessary sufficient for...
Background: We previously demonstrated that liver resection triggers estradiol production, which, in turn, induces the proliferation of hepatocytes to promote regeneration mice. In this study, we estradiol-induced estrogen receptor alpha (ERα) expression. Methods: To further explore role ERα estradiol-mediated regeneration, present confirmed impaired ability knockout Results: Further analysis during revealed a for hepatic steatosis, tumor necrosis factor-alpha and interleukin 6 expression,...
The SV40 capsid is composed primarily of 72 pentamers the VP1 major protein. Although also contains minor protein VP2 and its amino-terminally truncated form VP3, their roles in assembly remain unknown. An vitro system was used to investigate role recombinant pentamers. Under physiological salt pH conditions, alone remained dissociated, at 5.0, it assembled into tubular structures. A stoichiometric amount allowed spherical particles a range 7.0 4.0. Electron microscopy observation, sucrose...
The simian virus 40 (SV40) particle is mainly composed of the major capsid protein termed VP1. VP1 self‐assembles into virus‐like particles (VLPs) approximately nm in diameter when over‐expressed bacteria or insect cells, but purified does not form such a structure under physiological conditions, and thus, mechanism assembly well understood. Using highly assembly/disassembly system vitro , here we provide evidence that DNA factor contributes to 40‐nm spherical particles. At pH 5, for...
Thalidomide is a teratogen that causes multiple malformations in the developing baby through its interaction with cereblon (CRBN), substrate receptor subunit of CRL4 E3 ubiquitin ligase complex. CRBN was originally reported as gene associated autosomal recessive non-syndromic mild mental retardation. However, function during brain development remains largely unknown. Here we demonstrate promotes by facilitating proliferation neural stem cells (NSCs). Knockdown zebrafish embryos impaired and...