A5079482663- RNA Research and Splicing
- Genomics and Chromatin Dynamics
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Fungal and yeast genetics research
- Epigenetics and DNA Methylation
- DNA Repair Mechanisms
- Cancer-related gene regulation
- Plant Molecular Biology Research
- CRISPR and Genetic Engineering
- Ubiquitin and proteasome pathways
- Cancer-related molecular mechanisms research
- Biochemical and Molecular Research
- Plant Disease Resistance and Genetics
- Bioinformatics and Genomic Networks
- Bacterial Genetics and Biotechnology
- RNA regulation and disease
- Signaling Pathways in Disease
- Genetics and Neurodevelopmental Disorders
- Viral Infectious Diseases and Gene Expression in Insects
- Plant Virus Research Studies
- Virus-based gene therapy research
- DNA and Nucleic Acid Chemistry
- Protein Degradation and Inhibitors
- Protein Tyrosine Phosphatases
Harvard University
2016-2025
University of Utah
2018-2019
Hebrew University of Jerusalem
2007
University of British Columbia
2004
University of Toronto
2001-2004
Sungkyunkwan University
2001
Tokyo Institute of Technology
1998-2000
Osaka University
2000
Dana-Farber Cancer Institute
1999
Purdue University West Lafayette
1998
The activities of several mRNA processing factors are coupled to transcription through binding RNA polymerase II (Pol II). largest subunit Pol contains a repetitive carboxy-terminal domain (CTD) that becomes highly phosphorylated during transcription. mRNA-capping enzyme binds only CTD, whereas other may bind both and unphosphorylated forms. Capping occurs soon after initiation before events, raising the question whether capping components remain associated with complex they have modified 5′...
Tadashi Wada, Toshiyuki Takagi, Yuki Yamaguchi, Anwarul Ferdous, Takeshi Imai, Susumu Hirose, Seiji Sugimoto, Keiichi Yano, Grant A. Hartzog, Fred Winston, Stephen Buratowski, and Hiroshi Handa Faculty of Bioscience Biotechnology, Tokyo Institute Technology, Midori-ku, Yokohama 226, Japan; National Genetics, Mishima, Shizuoka-ken 411, Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., Machida-shi 194, Department Genetics Biological Chemistry Molecular Pharmacology, Harvard Medical School,...
Set2 methylates Lys36 of histone H3. We show here that yeast copurifies with RNA polymerase II (RNAPII). Chromatin immunoprecipitation analyses demonstrated and H3 methylation are associated the coding regions several genes were tested correlate active transcription. Both depend, as well, on Paf1 elongation factor complex. The C terminus Set2, which contains a WW domain, is also required for effective methylation. Deletion CTK1, encoding an RNAPII CTD kinase, prevents recruitment, suggesting...
Chromatin plays roles in processes governed by different time scales. To assay the dynamic behavior of chromatin living cells, we used genomic tiling arrays to measure histone H3 turnover G1-arrested Saccharomyces cerevisiae at single-nucleosome resolution over 4% genome, and lower (approximately 265 base pair) entire genome. We find that nucleosomes promoters are replaced more rapidly than coding regions replacement rates correlate with polymerase density. In addition, rapid is found known...
To physically characterize the web of interactions connecting Saccharomyces cerevisiae proteins suspected to be RNA polymerase II (RNAPII) elongation factors, subunits Spt4/Spt5 and Spt16/Pob3 (corresponding human DSIF FACT), Spt6, TFIIF (Tfg1, -2, -3), TFIIS, Rtf1, Elongator (Elp1, -3, -4, -5, -6) were affinity purified under conditions designed minimize loss associated polypeptides then identified by mass spectrometry. was discovered associate with three distinct complexes: histones;...
Covalent modification of histone proteins plays a role in virtually every process on eukaryotic DNA, from transcription to DNA repair. Many different residues can be covalently modified, and it has been suggested that these modifications occur great number independent, meaningful combinations. Published low-resolution microarray studies the combinatorial complexity patterns suffer confounding effects caused by averaging levels over multiple nucleosomes. To overcome this problem, we used...
Capping of mRNA occurs shortly after transcription initiation, preceding other processing events such as splicing and polyadenylation. To determine the mechanism coupling between capping, we tested for a physical interaction capping enzyme machinery. is not stably associated with basal factors or RNA polymerase II (Pol II) holoenzyme. However, can directly specifically interact phosphorylated form carboxy-terminal domain (CTD). This association in context initiation complex blocked by...
Cell size varies greatly between cell types, yet within a specific type and growth condition, is narrowly distributed. Why maintenance of cell-type important remains poorly understood. Here we show that growing budding yeast primary mammalian cells beyond certain impairs gene induction, cell-cycle progression, signaling. These defects are due to the inability large scale nucleic acid protein biosynthesis in accordance with volume increase, which effectively leads cytoplasm dilution. We...
The C-terminal domain (CTD) of the RNA polymerase II (Pol II) largest subunit is hyperphosphorylated during transcription. Using an in vivo cross-linking/chromatin immunoprecipitation assay, we found previously that different phosphorylated forms Pol predominate at stages At promoters, CTD Ser 5 by basal transcription factor TFIIH. However, coding regions, predominantly 2. Here show elongation-associated phosphorylation 2 dependent upon Ctk1 kinase, a putative yeast homolog Cdk9/P-TEFb....