A5101625166- Bacteriophages and microbial interactions
- Gut microbiota and health
- CRISPR and Genetic Engineering
- DNA Repair Mechanisms
- Photosynthetic Processes and Mechanisms
- RNA modifications and cancer
- Monoclonal and Polyclonal Antibodies Research
- SARS-CoV-2 and COVID-19 Research
- Metal-Catalyzed Oxygenation Mechanisms
- Genomics and Chromatin Dynamics
- Probiotics and Fermented Foods
- Pediatric health and respiratory diseases
- Bacterial Infections and Vaccines
- vaccines and immunoinformatics approaches
- S100 Proteins and Annexins
- Clostridium difficile and Clostridium perfringens research
- Plant Reproductive Biology
- Metalloenzymes and iron-sulfur proteins
- Clusterin in disease pathology
- Redox biology and oxidative stress
- Biochemical and Structural Characterization
- Gene expression and cancer classification
- Caveolin-1 and cellular processes
- Fungal and yeast genetics research
- Viral gastroenteritis research and epidemiology
Catholic University of America
2017-2025
First People's Hospital of Yunnan Province
2025
Kunming University of Science and Technology
2025
Sun Yat-sen University
2017-2024
University of America
2017-2022
Fifth Affiliated Hospital of Sun Yat-sen University
2021
University of Colorado Denver
2008-2017
Lanzhou University
2013-2017
Canada Research Chairs
2003
University of Toronto
2003
Bacteriophages likely constitute the largest biomass on Earth. However, very few phage genomes have been well-characterized, tailed T4 genome being one of them. Even in T4, much remained uncharacterized. The classical genetic strategies are tedious, compounded by modifications such as cytosine hydroxylmethylation and glucosylation which makes DNA resistant to most restriction endonucleases. Here, using type-II CRISPR-Cas9 system, we report editing both modified (ghm-Cytosine) unmodified...
Designing artificial viral vectors (AVVs) programmed with biomolecules that can enter human cells and carry out molecular repairs will have broad applications. Here, we describe an assembly-line approach to build AVVs by engineering the well-characterized structural components of bacteriophage T4. Starting a 120 × 86 nm capsid shell accommodate 171-Kbp DNA thousands protein copies, various combinations biomolecules, including DNAs, proteins, RNAs, ribonucleoproteins, are externally...
Phages show an elevated mutation rate and remarkably rapid evolution when attacked by the bacterial CRISPR/Cas system.
The regulation of pollen development and tube growth is a complicated biological process that crucial for sexual reproduction in flowering plants. Annexins are widely distributed from protists to higher eukaryotes play multiple roles numerous cellular events by acting as putative "linker" between Ca2+ signaling, the actin cytoskeleton membrane, which required growth. Our recent report suggested downregulation function Arabidopsis annexin 5 (Ann5) transgenic Ann5-RNAi lines caused severely...
According to the World Health Organization, COVID-19 may have caused ~15-million deaths across globe and is still ravaging world. Another wave of ~100 million infections predicted in United States due emergence highly transmissible immune-escaped Omicron variants.
Abstract Background Secondary bacterial infections and pneumonia are major mortality causes of respiratory viruses, the disruption upper tract (URT) microbiota is a crucial component this process. However, whether URT dysbiosis associates with viral species (in other words, type-specific) unclear. Results Here, we recruited 735 outpatients symptoms, identified infectious virus types in 349 participants using multiplex RT-PCR, profiled their microbiome 16S ribosomal RNA gene metagenomic...
Fidelity in DNA replication and repair requires adequate balanced deoxyribonucleotide pools that are maintained primarily by regulation of ribonucleotide reductase (RNR). RNR is controlled via transcription, protein inhibitor association, subcellular localization its two subunits, R1 R2. Saccharomyces cerevisiae Sml1 binds inhibits activity, while Schizosaccharomyces pombe Spd1 impedes holoenzyme formation sequestering R2 the nucleus away from cytoplasmic R1. Here we report identification...
The oral and upper respiratory tracts are closely linked anatomically physiologically with the lower tract lungs, influence of microbes on lung microbiota is increasingly being recognized. However, ecological process individual heterogeneity shaping remain unclear owing to lack controlled analyses sufficient sample sizes. Here, microbiomes saliva, nasal cavity, oropharyngeal area, bronchoalveolar lavage samples profiled multisource measured. It found that microbial inputs jointly shape by...
Bacteriophages (phages) are viruses that infect bacteria and use them as replication factories to assemble progeny phages. Bacteria have evolved powerful defense mechanisms destroy the invading phages by severing their genomes soon after entry into cells.
A multivalent, rapidly deployable, mucosal vaccine platform is desperately needed to prevent acquisition and transmission of respiratory infections during epidemics pandemics. No such approved currently exists virtually all under investigation use infectious viruses that have safety concerns are not amenable for multivalent engineering. Herein, a non‐infectious biomaterial presented, the bacteriophage T4 nanoparticle endowed with unique features modular engineering, which exploited design...
Related studies have pointed out that cell adhesion may play an important role for treating Polycystic Ovary Syndrome (PCOS). This study aimed to identify and analyze the biomarkers associated with adhesion-related genes (CRGs) PCOS their biological mechanisms. In this study, GSE80432 was used differentially expressed (DEGs) (PCOS vs control group) through differential expression analysis. Then, DEGs were overlapped 1531 CRGs obtain cross - genes. Subsequently, Support Vector...
Understanding the dynamics of lung microbiota in tuberculosis patients, especially those who cannot be confirmed bacteriologically clinical practice, is imperative for accurate diagnosis and effective treatment. This study aims to characterize distinct microbial features between negative patients understand influence on patients. We collected specimens bronchoalveolar lavage fluid from 123 Samples were subjected metagenomic next-generation sequencing reveal signatures. By combining...
Ribonucleotide reductase (RNR) is an essential iron-dependent enzyme that catalyzes deoxyribonucleotide synthesis in eukaryotes. Living organisms have developed multiple strategies to tightly modulate RNR function avoid inadequate or unbalanced pools cause DNA damage and genome instability. Yeast cells activate response genotoxic stress iron deficiency by facilitating redistribution of its small heterodimeric subunit Rnr2-Rnr4 from the nucleus cytoplasm, where it forms active holoenzyme with...
The detoxification ability and toxin binding properties (ability strength) of aflatoxin B1 by six strains probiotic bacteria were studied. the organisms ranged between 20-50%. About 60-80% bound was removed when washed with water, only 10-40% strongly to bacterial cells. Among studied, Bifidobacterium infantis 1912 demonstrated a significantly higher than other strains, while pseudolongum 20099 showed least about 10% added 50 ng B1. bifidum 1 strength during 180 min treatment.
A tightly controlled cellular deoxyribonucleotide (deoxynucleoside triphosphate [dNTP]) pool is critical for maintenance of genome integrity. One mode dNTP regulation through subcellular localization ribonucleotide reductase (RNR), the enzyme that catalyzes rate-limiting step biosynthesis. In Saccharomyces cerevisiae, RNR small subunit, Rnr2-Rnr4, localized to nucleus, whereas large Rnr1, cytoplasmic. As cells enter S phase or encounter DNA damage, Rnr2-Rnr4 relocalizes cytoplasm form an...
A multivalent, rapidly deployable, mucosal vaccine platform is desperately needed to prevent the acquisition and transmission of respiratory infections during epidemics pandemics. We present one such bacteriophage T4-based platform, design dual COVID-19-Flu vaccines by exploiting its unique architecture. These include: T4's natural affinity for nasal mucosa, flexible engineering incorporate multiple antigens, repeat symmetric epitope presentation enhanced B cell responses. Hundreds...