A5034622918- Pancreatic function and diabetes
- Protein Degradation and Inhibitors
- Metabolism, Diabetes, and Cancer
- SARS-CoV-2 and COVID-19 Research
- Pregnancy and preeclampsia studies
- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Reproductive System and Pregnancy
- Ubiquitin and proteasome pathways
- Neuroinflammation and Neurodegeneration Mechanisms
- Biochemical and Molecular Research
- Chronic Kidney Disease and Diabetes
- Immune cells in cancer
- Liver Disease Diagnosis and Treatment
- Peptidase Inhibition and Analysis
- Cytokine Signaling Pathways and Interactions
- Hepatitis C virus research
- Acute Kidney Injury Research
- interferon and immune responses
- Growth Hormone and Insulin-like Growth Factors
- FOXO transcription factor regulation
- Organ Transplantation Techniques and Outcomes
- Immune Response and Inflammation
- Axon Guidance and Neuronal Signaling
- Liver physiology and pathology
Shionogi (Japan)
2021-2024
National Center for Geriatrics and Gerontology
2019-2022
Gifu University
2022
Futaba (Japan)
2021
Nagoya University
2020
The University of Tokyo
2016
Kyoto University
2014
In the developing cortex, postmigratory neurons accumulate in cortical plate (CP) to properly differentiate consolidating subtype identities. Microglia, despite their extensive surveying activity, temporarily disappear from midembryonic CP. However, mechanism and significance of this absence are unknown. Here, we show that microglia bidirectionally migrate via attraction by CXCL12 released meninges subventricular zone thereby exit Upon nonphysiological excessive exposure vivo or vitro, young...
Background: We previously demonstrated that liver resection triggers estradiol production, which, in turn, induces the proliferation of hepatocytes to promote regeneration mice. In this study, we estradiol-induced estrogen receptor alpha (ERα) expression. Methods: To further explore role ERα estradiol-mediated regeneration, present confirmed impaired ability knockout Results: Further analysis during revealed a for hepatic steatosis, tumor necrosis factor-alpha and interleukin 6 expression,...
Multifaceted microglial functions in the developing brain, such as promoting differentiation of neural progenitors and contributing to positioning survival neurons, have been progressively revealed. Although previous studies noted relationship between vascular endothelial cells microglia little attention has given importance pericytes, mural surrounding cells. In this study, we attempted dissect role pericytes distribution function mouse brains. Our immunohistochemical analysis showed that...
Abstract SARS-CoV-2 pandemic alerts us that spillovers of various animal coronaviruses to human in the future may bring enormous damages. Thus, there is a significant need antibody-based drugs treat patients infected with previously unseen coronaviruses.CV804 against S2 domain spike protein, which less prone mutations. CV804 shows not only broad cross-reactivities representative 20 animal-origin but also diseases-associated beta including SARS-CoV, MERS-CoV, HCoV-OC43, HCoV-HKU1 and mutant...
Several viruses are known to infect human liver and cause the hepatitis, but interferon (IFN) response, a first-line defense against viral infection, of virus-infected hepatocytes is not clearly defined yet. We investigated innate immune system RNA infection in immortalized (HuS-E/2 cells), as cells showed similar early responses primary (PHH). The low-level constitutive expression IFN-α1 gene, IFN-β IFN-λ, was observed both PHH HuS-E/2 absence suggesting particular subtype(s) IFN-α...
We previously reported that glucokinase is ubiquitinated and degraded by cereblon with an unknown endogenous protein degrader. Here, we show UDP-glucose a identified both glucose bind to uridine in similar way thalidomide. From these results, was as molecular glue between glucokinase. Glucokinase produces glucose-6-phosphate the pancreas liver. Especially β-cells, main target of for glucose-induced insulin secretion. administration glucokinase, lowered production, then reduced secretion...
Abstract Insulin secretion is regulated in multiple steps, and one of the main steps endoplasmic reticulum (ER). Here, we show that UDP-glucose induces proinsulin ubiquitination by cereblon, uridine binds competes for degradation behaves as sustainable insulin secretagogue. Using mutagenesis neonatal diabetes variant-C43G maturity-onset young 10 (MODY10) variant-R46Q, UDP-glucose:glycoprotein glucosyltransferase 1 (UGGT1) protects cereblon-dependent ER. Cereblon a ligand-inducible E3...
The SARS-CoV-2 pandemic alerted the potential for significant harm due to future cross-species transmission of various animal coronaviruses human. There is a need antibody-based drugs treat patients infected with previously unseen coronaviruses. In this study, we generated CV804, an antibody that binds S2 domain spike protein, which highly conserved across coronavirus family and less susceptible mutations. CV804 demonstrated broad cross-reactivities not only disease-associated human beta...
Abstract Insulin secretion is regulated in multiple steps, and one of the main steps endoplasmic reticulum (ER). Here, we show that UDP-glucose induces proinsulin ubiquitination by cereblon (CRBN), uridine binds competes for degradation behaves as sustainable insulin secretagogue. Using mutagenesis neonatal diabetes variant-C43G maturity-onset young 10 (MODY10) variant-R46Q, UDP-glucose:glycoprotein glucosyltransferase 1 (UGGT1) protects CRBN-dependent ER. CRBN a ligand-inducible E3...
Abstract Arginine releases proinsulin from binding to UGGT1 in the endoplasmic reticulum (ER). PSIPRED analysis showed that arginine/proinsulin domain (A/PBD) C-terminal region of forms a disordered region, which is flexible and outside main protein structure. may easily access UGGT1. Using SNP library, two variants, Q1518* R1526C, were identified this region. variant deficient form A/PBD ER-retention signal (ERRS). mutated cell reveals mainly secreted protein, because lack ERRS. We found...