A5059217524- Carbohydrate Chemistry and Synthesis
- Glycosylation and Glycoproteins Research
- Antimicrobial Peptides and Activities
- Enzyme Production and Characterization
- Chemical Synthesis and Analysis
- Click Chemistry and Applications
- Biochemical and Structural Characterization
- Monoclonal and Polyclonal Antibodies Research
- Microbial Metabolites in Food Biotechnology
- Biochemical and Molecular Research
- Antimicrobial agents and applications
- Antibiotic Resistance in Bacteria
- Bacteriophages and microbial interactions
- Viral gastroenteritis research and epidemiology
- Polysaccharides and Plant Cell Walls
- Microfluidic and Capillary Electrophoresis Applications
- Drug Solubulity and Delivery Systems
- biodegradable polymer synthesis and properties
- Analytical Chemistry and Chromatography
- Escherichia coli research studies
- Machine Learning in Bioinformatics
- Protein purification and stability
- Lipid Membrane Structure and Behavior
- Aortic Disease and Treatment Approaches
- Innovative Microfluidic and Catalytic Techniques Innovation
Nagoya Institute of Technology
2016-2025
Graduate School USA
2019
Kochi University of Technology
2010
RIKEN Advanced Science Institute
2010
Japan Chemical Innovation and Inspection Institute
2008-2009
The University of Tokyo
2004-2009
Collaborative Research Group
2008
Osaka University
2006
Saitama University
2004-2006
Shimane University
2004
Abstract Volatiles from herbivore-infested plants function as a chemical warning of future herbivory for neighboring plants. ( Z )-3-Hexenol emitted tomato infested by common cutworms is taken up uninfested and converted to )-3-hexenyl β-vicianoside (HexVic). Here we show that wild species Solanum pennellii ) shows limited HexVic accumulation compared domesticated lycopersicum after )-3-hexenol exposure. Common grow better on an introgression line containing S. chromosome 11 segment impairs...
Shiga toxin (Stx) is a major virulence factor in infection with Stx-producing Escherichia coli (STEC). We developed series of linear polymers acrylamide, each different density trisaccharide globotriaosylceramide (Gb3), which receptor for Stx, and identified Gb3 highly clustered trisaccharides as Stx adsorbents functioning the gut. The specifically bound to both Stx1 Stx2 high affinity markedly inhibited cytotoxic activities these toxins. Oral administration protected mice after fatal dose...
ABSTRACT Maltose‐binding protein (MBP) is used as affinity tags for purifying recombinant proteins via amylose gels. However, the gel often yields a low quantity of purified due to biodegradability and water solubility immobilized amylose. Herein, water‐soluble, anti‐biodegradation amylose‐mimicking polymers are synthesized by polymerizing maltose monomer with norbornene group, then copolymerizing it glucose norbornene. The further modified hydrogenation hydroxylation alter their properties....
Antimicrobial peptides that act by disrupting bacterial membranes are attractive agents for treating drug-resistant bacteria. This study investigates a membrane-disrupting peptide mimic made of cyclic oligosaccharide cyclodextrin scaffold can be chemically polyfunctionalized. An antibacterial functional group on the was simplified to an alkylamino combines cationic and hydrophobic moieties, former interact with anionic membrane latter interior. The cyclodextrins equipped eight groups...
We previously developed linear polymers bearing clustered trisaccharides of globotriaosylceramide (Gb3) as orally applicable Shiga toxin (Stx) neutralizers. Here, using a Gb3 polymer with short spacer tethering the trisaccharide to core, we found that shortening length markedly reduced binding affinity for Stx2 but not Stx1. Moreover, mutational analysis revealed essential sites terminal were completely different between Stx1 and Stx2. These results provide molecular basis interaction Stx B...
Cyclodextrin derivatives prepared to mimic a membrane active antibacterial peptide polymyxin B strongly permeabilized bacterial and inhibited proliferation.
Cyclodextrin derivatives are synthesized as membrane-disrupting agents via a microwave-assisted Huisgen reaction. Their ability to permeabilize bacterial membranes depends on the amino substituents and an appropriate balance of hydrophobicity hydrophilicity, thus enabling preparation with selective toxicity against bacteria.
To convert coal fly ash (Fa) into Na-A zeolites, Fa and NaOH−NaAlO2 solutions were added the tube made by a semipermeable membrane aged in same at 85 °C for given period. The amorphous aluminosilicates used as Si Al sources synthesis of zeolites. SiO2/Al2O3 molar ratio starting materials was controlled from 2.0 to 5.0. aluminosilicate completely dissolved during aging Pc type zeolite formed over whole range SiO2/Al2O3. On other hand, crystalline phase Fa, such α-quartz mullite, poorly...
Abstract Emergence of drug‐resistant bacterial pathogens and the concurrent demand for new antibiotics has led to membrane‐active antimicrobial cyclodextrin (CD) development. CDs contain polyalkylamino groups; molecule polyfunctionalization was achieved via a click reaction. A survey using with systematically varied functionalities clarified unique correlation their activity molecules’ hydrophobicity/hydrophilicity balance. The optimum hydrophobicity specific strains animal cells, leading...
Colistin-like poly-amino β-cyclodextrin molecules locally possessing a hydrophobic alkyl chain tail disrupted bacterial membranes without destroying animal cells, resulting in achievement of selective anti-bacterial activity.
We previously reported that glucokinase is ubiquitinated and degraded by cereblon with an unknown endogenous protein degrader. Here, we show UDP-glucose a identified both glucose bind to uridine in similar way thalidomide. From these results, was as molecular glue between glucokinase. Glucokinase produces glucose-6-phosphate the pancreas liver. Especially β-cells, main target of for glucose-induced insulin secretion. administration glucokinase, lowered production, then reduced secretion...
β-(1,3)-Glucan has been used as an anti-cancer drug owing to its immunostimulatory effects. However, the mechanism of biological activity yet be elucidated. Moreover, β-(1,3)-glucans for mechanistic studies are heterogeneous mixtures extracted from natural sources. Therefore, structure-defined required simplify effect caused by in study activity, and this requirement prompts chemical synthesis β-(1,3)-glucans. The glycosylation formation β-(1,3)-linkage is difficult because reactivity...
Abstract The chemical polymodification of cyclodextrins and amyloses proceeded quickly with use the microwave-assisted Huisgen reaction in an all-or-none fashion without formation less-modified by-products.
The modified cyclodextrins exhibited antimicrobial activity against drug-resistant bacteria and less hemolytic activity.
Peptide gemini-surfactant (PG-surfactant), a kind of lipopeptide, is composed short linker peptide (X) between two alkyl-chain-modified Cys residues and peripheral peptides at the N-terminal (Y) C-terminal (Z) sides, respectively, alkylated residues. In this study, we developed examined series PG-surfactants containing C12 saturated alkanes oligo-Lys, arranged X-, Y-, or Z-positions. To arrange oligo-Lys Y- Z-positions, repeat sequence -Asp-Lys-Asp-Lys- was used X-position. All exhibited...
The trityl group is an important and useful protecting for primary hydroxy groups on carbohydrates. However, during deprotection, neighboring acetyl can easily migrate to the deprotected groups. Hence, deprotection of was optimized using a microreactor with regard flow rate, reagent concentration, reaction time, substrate concentration. microflow conditions inhibited migration could be applied large-scale reactions other substrates.